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  4. Recombinant Human RISC-loading complex subunit TARBP2 (TARBP2)

Recombinant Human RISC-loading complex subunit TARBP2 (TARBP2)

  • 中文名称：
    人TARBP2重组蛋白
  • 货号：
    CSB-EP623007HU
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    TARBP2
  • Uniprot No.：
  • 别名：
    LOQS; Prbp; RISC loading complex subunit TARBP2; RISC-loading complex subunit tarbp2; TAR (HIV 1) RNA binding protein 2; TAR (HIV) RNA binding protein 2; TAR (HIV) RNA-binding protein TRBP1; TAR RNA Binding Protein 2; TAR RNA-binding protein 2; tarbp2; TARBP2 RISC loading complex RNA binding subunit; Trans-activation-responsive RNA-binding protein; TRBP; TRBP1; TRBP2; TRBP2_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    66.0kDa
  • 表达区域：
    1-366aa
  • 氨基酸序列
    MSEEEQGSGTTTGCGLPSIEQMLAANPGKTPISLLQEYGTRIGKTPVYDLLKAEGQAHQPNFTFRVTVGDTSCTGQGPSKKAAKHKAAEVALKHLKGGSMLEPALEDSSSFSPLDSSLPEDIPVFTAAAAATPVPSVVLTRSPPMELQPPVSPQQSECNPVGALQELVVQKGWRLPEYTVTQESGPAHRKEFTMTCRVERFIEIGSGTSKKLAKRNAAAKMLLRVHTVPLDARDGNEVEPDDDHFSIGVGSRLDGLRNRGPGCTWDSLRNSVGEKILSLRSCSLGSLGALGPACCRVLSELSEEQAFHVSYLDIEELSLSGLCQCLVELSTQPATVCHGSATTREAARGEAARRALQYLKIMAGSK
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal GST-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. May also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1.; (Microbial infection) Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. This is achieved in part at least by binding to and inhibiting EIF2AK2/PKR, thereby reducing phosphorylation and inhibition of EIF2S1/eIF-2-alpha. May also promote translation of TAR-containing RNAs independently of EIF2AK2/PKR. Mediates recruitment of FTSJ3 methyltransferase to HIV-1 RNA, leading to 2'-O-methylation of the viral genome, allowing HIV-1 to escape the innate immune system.
  • 基因功能参考文献：
    1. These results establish that PKR regulation through stress-induced TRBP phosphorylation is an important mechanism ensuring cellular recovery and preventing apoptosis due to sustained PKR activation. PMID: 29348664
    2. A statistically significant association with the risk of AD was observed with the CT genotype for rs784567 on the TARBP2 gene. on controlling for age and sex, we found that for the TARBP2-RNASEN association with AD the age variation was a risk factor for AD risk (P<0.001; OR=1.104; 95% CI, 1.059-1.151). PMID: 26796812
    3. TRBP is a novel transcriptional coactivator of the Notch signaling pathway, playing an important role in neural stem cell regulation during mammalian brain development PMID: 28174252
    4. TRBP is required for normal antiviral responses to Cardiovirus infection. PMID: 27743889
    5. Experimental and computational techniques were employed to examine the hypothesis that TRBP-double-stranded RNA-binding domain (dsRBD) 2 binding by a 20-mer dsRNA is coupled with RNA bending, results suggest that this particular dsRBD-dsRNA interaction produces little to no change in the A-form geometry of dsRNA in solution PMID: 27332119
    6. We propose that the S6K2/TRBP node controls miRNA biogenesis in HDLECs and provides a molecular link between the mTOR pathway and the miRNA biogenesis machinery. PMID: 27407113
    7. TARBP2 silencing inhibits in vitro invasion and migration of NCIH1299 nonsmall cell lung cancer cell via the JNK/STAT3/AKT pathway. TARBP2 silencing does not significantly affect cell growth. PMID: 27599909
    8. TARBP2 has roles in tumor development and progression [review] PMID: 26486325
    9. SUMOylation of TARBP2 is required for regulating siRNA efficiency. PMID: 26582366
    10. HIV-1 Rev-response element RNA acts as an RNA silencing suppressor by competing with TRBP-bound siRNAs. PMID: 25668122
    11. the role of TRBP and unveils negative feedback regulation of PKR through TRBP phosphorylation. PMID: 25437560
    12. Results show the crystal structure of the interface between microRNA biogenesis proteins Dicer and TRBP. Mutations in this interface prevent recruitment of TRBP to Dicer. PMID: 25557550
    13. RPL5 binds to the TRBP2 and Ago2 subunits of the RISC and mediates the binding to c-Myc 3'UTR. PMID: 24141778
    14. TARBP2 expression is upregulated in late-stage breast cancer and triple-negative breast cancer and may have a role in poorer disease-free survival and overall survival PMID: 24563327
    15. identified TARBP2, a double-stranded RNA-binding protein implicated in microRNA processing, as the trans factor that binds the sRSE family and similar structural elements--collectively termed TARBP2-binding structural elements (TBSEs)--in transcripts PMID: 25043050
    16. Tarbp2 binding to TRPC4 promotes changes of cytosolic Ca(2+) and, thereby, leads to a dynamic regulation of Dicer activity, essentially at low endogenous Dicer concentrations. PMID: 24563462
    17. mRNA expression of TARBP2, but not DICER or DROSHA, is a strong molecular predictor to discriminate between adrenocortical adenomas and carcinomas PMID: 23671264
    18. in vitro binding patterns of human TRBP and PACT to siRNA PMID: 23658827
    19. The results show that PACT and TRBP have distinct effects on Dicer-mediated dsRNA processing. PMID: 23661684
    20. No frameshift mutations in TARBP2 were identified in a study of 4 cases of upper urinary tract urothelial carcinoma with high microsatellite instability status. PMID: 23690119
    21. Results show that the region of TRBP that binds immature miRNAs comprises two independent double-stranded RNA-binding domains connected by a 60-residue flexible linker. PMID: 23435228
    22. RISC proteins PACT, TRBP, and Dicer, together with PKR, are steroid receptor RNA activator-binding nuclear receptor coregulators that are recruited to the promoters of hormone-regulated genes and regulate the expression of downstream target genes. PMID: 23550157
    23. We report an unanticipated dsRNA diffusion behavior of TRBP, which requires two double-stranded RNA binding domains, and its correlation with the role of TRBP in promoting Dicer-induced RNA cleavage. PMID: 23251028
    24. The studies in this review show that TRBP is multifunctional and mediates cross talk between different pathways. Its activities at the molecular level impact the cellular function from normal development to cancer and the response to infections. PMID: 22933564
    25. There was no significant difference in the TARBP2 expression levels between the epithelial skin cancer groups and the healthy controls (P > 0.05). PMID: 22025453
    26. Depletion of Dicer and TRBP, proteins involved in miRNA biogenesis, reduced HCV RNA accumulation, mature duplex miR-122 abundance, and miR-122 directed mRNA translation suppression, suggesting roles in miR-122 processing. PMID: 22999255
    27. TRBP is a key regulator of miRNA processing and targeting in humans. PMID: 23006623
    28. Data suggest that cancer stem cell (CSC) self-renewal and tumor maintenance may depend on deregulation of TARBP2-dependent miRNA expression. PMID: 22698405
    29. AGO2, PACT and TRBP are required for the efficient functioning of Dicer in cells PMID: 22163034
    30. results demonstrate for the first time that stress-induced PACT phosphorylation functions to free PACT from the inhibitory interaction with TRBP and also to enhance its interaction with PKR PMID: 21526770
    31. The structures dsRNA-bdinding domain 1 and dsRNA binding domain 2 solved by x-ray crystallography and NMR spectroscopy respectively. PMID: 21080422
    32. TARBP2 frameshift mutation is associated with hereditary nonpolyposis colorectal cancer. PMID: 20877318
    33. the three-dimensional structure of human Dicer bound to the protein TRBP at approximately 20 A resolution determined by negative-stain electron microscopy (EM) and single-particle analysis PMID: 19836333
    34. binds to merlin, which inhibits its oncogenic activity; results provide the first clue to the functional interaction between TRBP and merlin and suggest a novel mechanism for the tumor suppressor function of merlin both in vitro and in vivo PMID: 15123692
    35. Data suggest that the cell specifity of HIV-1 expression and replication may be regulated, in part, through the control of TRBP1 expression by NF-Y factors. PMID: 16343534
    36. These results suggest that, in the context of HIV replication, TRBP contributes mainly to the enhancement of virus production and that Dicer does not mediate HIV restriction by RNAi. PMID: 17360756
    37. Results indicate that human TRBP and PACT directly interact with each other and associate with Dicer to stimulate the cleavage of double-stranded or short hairpin RNA to siRNA. PMID: 17452327
    38. Ago2, Dicer, and TRBP comprise the RISC-loading complex (RLC) and assembles spontaneously in vitro from purified components PMID: 18178619
    39. TRBP controls PACT activation of PKR, an activity that is reversed by stress. PMID: 18936160
    40. RDE-4 preferentially binds long dsRNA, while TRBP binds siRNA with an affinity that is independent of dsRNA length. PMID: 18948111
    41. The RLC Dicer's N-terminal DExH/D domain, located in a short 'base branch', interacts with TRBP, whereas its C-terminal catalytic domains in the main body are proximal to AGO2. PMID: 19820710

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  • 亚细胞定位：
    Cytoplasm. Cytoplasm, perinuclear region. Nucleus.
  • 蛋白家族：
    TARBP2 family
  • 数据库链接：

    HGNC: 11569

    OMIM: 605053

    KEGG: hsa:6895

    STRING: 9606.ENSP00000266987

    UniGene: Hs.326