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  4. Recombinant Human Rac GTPase-activating protein 1 (RACGAP1)

Recombinant Human Rac GTPase-activating protein 1 (RACGAP1)

  • 中文名称：
    人RACGAP1重组蛋白
  • 货号：
    CSB-YP884432HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人RACGAP1重组蛋白
  • 货号：
    CSB-EP884432HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人RACGAP1重组蛋白
  • 货号：
    CSB-EP884432HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人RACGAP1重组蛋白
  • 货号：
    CSB-BP884432HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人RACGAP1重组蛋白
  • 货号：
    CSB-MP884432HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    RACGAP1
  • Uniprot No.：
  • 别名：
    CYK4; GAP; Gap1; GTPase activating protein; HsCYK-4; ID GAP; KIAA1478; Male germ cell RacGap; MgcRacGAP; Protein CYK4 homolg; Protein CYK4 homolog; Rac GTPase activating protein 1; Rac GTPase-activating protein 1; RACGAP 1; Racgap1; RGAP1_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-632
  • 氨基酸序列
    MDTMMLNVRN LFEQLVRRVE ILSEGNEVQF IQLAKDFEDF RKKWQRTDHE LGKYKDLLMK AETERSALDV KLKHARNQVD VEIKRRQRAE ADCEKLERQI QLIREMLMCD TSGSIQLSEE QKSALAFLNR GQPSSSNAGN KRLSTIDESG SILSDISFDK TDESLDWDSS LVKTFKLKKR EKRRSTSRQF VDGPPGPVKK TRSIGSAVDQ GNESIVAKTT VTVPNDGGPI EAVSTIETVP YWTRSRRKTG TLQPWNSDST LNSRQLEPRT ETDSVGTPQS NGGMRLHDFV SKTVIKPESC VPCGKRIKFG KLSLKCRDCR VVSHPECRDR CPLPCIPTLI GTPVKIGEGM LADFVSQTSP MIPSIVVHCV NEIEQRGLTE TGLYRISGCD RTVKELKEKF LRVKTVPLLS KVDDIHAICS LLKDFLRNLK EPLLTFRLNR AFMEAAEITD EDNSIAAMYQ AVGELPQANR DTLAFLMIHL QRVAQSPHTK MDVANLAKVF GPTIVAHAVP NPDPVTMLQD IKRQPKVVER LLSLPLEYWS QFMMVEQENI DPLHVIENSN AFSTPQTPDI KVSLLGPVTT PEHQLLKTPS SSSLSQRVRS TLTKNTPRFG SKSKSATNLG RQGNFFASPM LK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Required for proper attachment of the midbody to the cell membrane during cytokinesis. Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity. Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation. Shows strong GAP (GTPase activation) activity towards CDC42 and RAC1 and less towards RHOA. Essential for the early stages of embryogenesis. May play a role in regulating cortical activity through RHOA during cytokinesis. May participate in the regulation of sulfate transport in male germ cells.
  • 基因功能参考文献：
    1. RACGAP1 promotes proliferation of hepatocellular carcinoma cells by reducing activation of the Hippo and YAP pathways and promoting cytokinesis in coordination with nuclear basket protein, TPR. PMID: 30009820
    2. The rs615382 variant disrupts the recombination signal binding protein with immunoglobulin kappa J binding site in Rac GTPase activating protein 1. Disruption of notch 1 mediated-repression of RACGAP1 may contribute to Prostate Cancer in African Americans. PMID: 29695400
    3. Study showed that a subset of epithelial ovarian cancer (EOC) patients have RacGAP1 overexpression which is associated with advanced tumor stages and poor clinical outcomes. Also, RacGAP1 can positively regulate the activation of RhoA and Erk proteins, thus up-enhance the migration and invasion process of EOC. PMID: 29095547
    4. Data show that comparing with Ki-67 and TOP2A, RacGAP1 allowed for a clearer prognostic statement. PMID: 27259241
    5. RACGAP1 promotes the metastatic phenotype in uterine carcinosarcoma via a STAT3/survivin signaling pathway. PMID: 27121792
    6. High RACGAP1 expression is associated with Basal-like Breast Cancers. PMID: 27216196
    7. This study showed that the overexpressions of Ki67, RacGAP1, and TOP2a affect the prognosis of female breast cancer patients adversely PMID: 27284123
    8. Data confirmed a strong correlation of AURKA and Wnt-modulator RACGAP1 gene expression both in the gastric tumor, the tumor-adjacent and the tumor-distant mucosa. PMID: 26778597
    9. RacGAP1 Is a Novel Downstream Effector of E2F7-Dependent Resistance to Doxorubicin and Is Prognostic for Overall Survival in Squamous Cell Carcinoma PMID: 26018753
    10. RACGAP1 expression levels in the nucleus and cytoplasm, determined by immunohistochemical staining, predict opposite clinical outcomes and that both could be independent prognostic markers for colorectal cancer. PMID: 26508373
    11. central spindle assembly and 2 Plk1-dependent phosphorylations are required to establish efficient binding of the Ect2 BRCT in early cytokinesis. PMID: 25486482
    12. RacGAP1 expression at the invasive front in gastric cancer was significantly correlated with factors reflecting tumor progression and poor prognosis.RACGAP1 gene expression in diffuse type gastric cancer was elevated. PMID: 24615626
    13. RacGAP1 mediated endothelial barrier function loss and melanoma transmigration in a focal adhesion-dependent manner.RacGAP1 activation mediates focal adhesion assembly and actin stress fiber formation. PMID: 25475728
    14. High RacGAP1 expression was an independent predictive marker for lymph node metastasis, recurrence and poor prognosis in colorectal cancer patients. PMID: 25568185
    15. HCV viral protein NS5B polymerase activity was significantly reduced by silencing RacGAP1 and, vice versa, was increased by overexpression of RacGAP1 in a cell-based reporter assay. PMID: 25305482
    16. We show that some are new substrates of the anaphase-promoting complex/cyclosome and validate KIFC1 and RacGAP1/Cyk4 as two such targets involved respectively in timely mitotic spindle disassembly and cell spreading PMID: 24857844
    17. MgcRacGAP colocalizes with CGN and CGNL1 at TJs and forms a complex and interacts directly in vitro with CGN and CGNL1. PMID: 24807907
    18. High expression of RACGAP1 is associated with meningiomas. PMID: 23525949
    19. APC(CDH1) targets MgcRacGAP for destruction in the late M phase. PMID: 23696789
    20. The crystal structure of the GAP domain of MgcRacGAP has been determined at a resolution of 1.9A; MgcRacGAP (residues 348-546) exists as a monomer in solution. PMID: 23665020
    21. In untreated patients, Ki67, TOP2A, and RacGAP1 are significant and independent prognostic markers. PMID: 23135572
    22. High RACGAP1 mRNA expression (above the median) was associated with poor disease-free survival and overall survival in high risk early breast cancer PMID: 23096218
    23. MKlp1-CYK4 centralspindlin complex is a guanosine triphosphatase-activating protein (GAP) for Rac1 and not RhoA. PMID: 22945935
    24. several Rho family small GTPases activate PI3K by an indirect cooperative positive feedback that required a combination of Rac, CDC42, and RhoG small GTPase activities PMID: 22683270
    25. the level of RACGAP1 expression is sufficient to predict the early recurrence of HCC. PMID: 21825042
    26. The plexin-B1/Rac interaction inhibits PAK activation and enhances Sema4D ligand binding PMID: 11937491
    27. This enzyme is down-regulated by estradiol. PMID: 12493759
    28. Expression of a GTPase-activating protein (GAP)-deficient mutant (R386A) of MgcRacGAP induces abnormal cortical activity during cytokinesis in U2OS cells. Multiple large blebs were observed in cells expressing MgcRacGAP R386A. PMID: 14729465
    29. data reveal an important role for MgcRacGAP in STAT3 activation, and demonstrate that MgcRacGAP regulates IL-6-induced cellular differentiation in which STAT3 plays a pivotal role PMID: 15284113
    30. MgcRacGAP regulates the activation and function of Cdc42 in mitosis. PMID: 15642749
    31. H-Ras-specific activation of Rac-MKK3/6-p38 pathway has a role in invasion and migration of breast epithelial cells PMID: 15677464
    32. intracellular Ca2+ is mobilized by sphingosine 1 phosphate and mediates rac activation and adherens junction assembly in endothelial cells PMID: 15728185
    33. beta 2 chimerin has a role in rac-GAP-dependent inhibition of breast cancer cell proliferation PMID: 15863513
    34. activation of Vav1-Rac signaling pathway by CXCL12 represents an important inside-out event controlling efficient up-regulation of alpha4beta1-dependent T lymphocyte adhesion PMID: 15872091
    35. Cdk1 inactivation is sufficient to activate a signaling pathway leading to cytokinesis, which emanates from mitotic spindles and is regulated by ECT2, MgcRacGAP, and RhoA PMID: 16118207
    36. MgcRacGAP controls the initiation of cytokinesis by regulating ECT2, which in turn induces the assembly of the contractile ring and triggers the ingression of the cleavage furrow PMID: 16129829
    37. This study defines a cellular mechanism that links centralspindlin to Cep55, which, in turn, controls the midbody structure and membrane fusion at the terminal stage of cytokinesis. PMID: 16790497
    38. These findings demonstrate that HIF-1alpha function is negatively affected by its interaction with MgcRacGAP. PMID: 17982282
    39. report here that (i) MgcRacGAP is phosphorylated by Aurora B and Cdk1, (ii) PP2A dephosphorylates Aurora B and Cdk1 phosphorylated sites and (iii) inhibition of PP2A abrogates MgcRacGAP/Ect2 interaction PMID: 18201571
    40. Study demonstrates that FIP3 and ECT2 form mutually exclusive complexes with Cyk-4 and that dissociation of ECT2 from the mid-body at late telophase may be required for the recruitment of FIP3 and recycling endosomes to the cleavage furrow. PMID: 18511905
    41. study reports that mitotic complex genes Ect2, RacGAP, and MKLP1 are coordinately induced in S phase in proliferating T lymphocytes as well as in epithelial cells, depending upon activity of the CUX1 and E2F1 transcription factors PMID: 19015243
    42. MKLP1 interacts with CYK4, a GTPase activating protein for the Rho GTPase, to form the centralspindlin complex. Centralspindlin is a key regulator of Rho function and microtubule organisation during cytokinesis, and is essential for cell division. PMID: 11782313

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Cytoplasmic vesicle, secretory vesicle, acrosome. Cleavage furrow. Midbody, Midbody ring. Cell membrane; Peripheral membrane protein; Cytoplasmic side.
  • 组织特异性：
    Highly expressed in testis, thymus and placenta. Expressed at lower levels in spleen and peripheral blood lymphocytes. In testis, expression is restricted to germ cells with the highest levels of expression found in spermatocytes. Expression is regulated
  • 数据库链接：

    HGNC: 9804

    OMIM: 604980

    KEGG: hsa:29127

    STRING: 9606.ENSP00000309871

    UniGene: Hs.505469