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  4. Recombinant Human Ras association domain-containing protein 2 (RASSF2)

Recombinant Human Ras association domain-containing protein 2 (RASSF2)

  • 货号：
    CSB-YP019374HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP019374HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP019374HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP019374HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP019374HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    RASSF2
  • Uniprot No.：
  • 别名：
    CENP 34; Centromere protein 34; Ras association (RalGDS/AF 6) domain family 2; Ras association (RalGDS/AF-6) domain family member 2; Ras association domain containing protein 2; Ras association domain-containing protein 2; RASF2_HUMAN; RASFADIN; RASSF2; RP4-599I11.1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-326
  • 氨基酸序列
    MDYSHQTSLV PCGQDKYISK NELLLHLKTY NLYYEGQNLQ LRHREEEDEF IVEGLLNISW GLRRPIRLQM QDDNERIRPP PSSSSWHSGC NLGAQGTTLK PLTVPKVQIS EVDAPPEGDQ MPSSTDSRGL KPLQEDTPQL MRTRSDVGVR RRGNVRTPSD QRRIRRHRFS INGHFYNHKT SVFTPAYGSV TNVRINSTMT TPQVLKLLLN KFKIENSAEE FALYVVHTSG EKQKLKATDY PLIARILQGP CEQISKVFLM EKDQVEEVTY DVAQYIKFEM PVLKSFIQKL QEEEDREVKK LMRKYTVLRL MIRQRLEEIA ETPATI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Potential tumor suppressor. Acts as a KRAS-specific effector protein. May promote apoptosis and cell cycle arrest. Stabilizes STK3/MST2 by protecting it from proteasomal degradation.
  • 基因功能参考文献：
    1. Results showed that in the endometrial adenocarcinoma (OEA group) and ovarian clear cell carcinoma (OCC group) groups, the methylation degrees of the RASSF2A promoter were obviously higher than that of the other two groups. The expression level of RASSF2A mRNA in the OEA and OCC groups was lower than that of the other two groups. PMID: 29504361
    2. we confirmed that the RASSF2-PAR-4 axis was mainly responsible for miR-7 functions in CAFs using bioinformatics methods. Overexpression of miR-7 in CAFs led to down-regulation of RASSF2, which dramatically decreased the secretion of PAR-4 from CAFs and then enhanced the proliferation and migration of the co-cultured cancer cells. PMID: 27901488
    3. Results suggest that down-regulation of RASSF2, and RASSF6 is a tumor-specific phenomenon and their inactivation through CpG island methylation may play important roles in gastric cardia carcinogenesis. PMID: 26456015
    4. The statistically most significant association rs4813720 correlates with RASSF2 expression and may play a significant role in childhood ALL etiology. PMID: 27861356
    5. RASSF2 may be an important molecular marker for carcinogenesis, prognosis and progression in gastric cancer PMID: 26459248
    6. RASSF2 gene is differently methylated in luminal and non-luminal tumors and is a promising suppressor gene with clinical involvement in breast cancer PMID: 26284587
    7. our results suggest that RASSF2 may function as a tumor suppressor gene PMID: 26482475
    8. We conclude that EPB41L3, RASSF2 and TSP-1 genes are involved in the pathogenesis of diffuse gliomas PMID: 25621889
    9. Promoter hypermethylation of RASSF2A is observed in cervical cancer, while not in normal cervical tissues. PMID: 24605823
    10. Epigenetic inactivation of RASSF2A through aberrant promoter methylation may play an important role in the pathogenesis of epithelial ovarian cancer. PMID: 24606436
    11. results suggest that both RASSF1A and RASSF2 are novel epigenetically inactivated tumor suppressor genes in Ewing sarcoma and RASSF2 methylation may have prognostic implications for ES patients PMID: 23887284
    12. our results suggest that RASSF2 potentially functions as a new tumor-suppressor gene that is inactivated through hypermethylation in cervical cancer PMID: 23542458
    13. Promoter hypermethylation of RASSF2A is observed in pancreatic ductal adenocarcinoma, while not in normal pancreatic tissue. PMID: 21792082
    14. Hypermethylation of RASSF2 gene is associated with advanced gastric cancer. PMID: 21409489
    15. Sp1-mediated RASSF2A gene transcription is activated by p300 through histone acetylation, and this activation plays an important role in inducing late apoptosis. PMID: 20716062
    16. Results suggest that RASSF2 encodes a novel epigenetically inactivated candidate tumor suppressor gene in thyroid carcinogenesis. PMID: 20920251
    17. Aberrant methylation of the RASSF2A gene with the subsequent loss of RASSF2A expression plays an important role in the pathogenesis of hepatocellular carcinoma. PMID: 19513612
    18. Data show that RASSF2 forms a direct and endogenous complex with prostate apoptosis response protein 4 (PAR-4) and that this interaction is regulated by K-Ras and is essential for the full apoptotic effects of PAR-4. PMID: 20368356
    19. these findings indicate that MST1 is a major determinant of RASSF2 protein stability, and suggest that RASSF2 acts in a complex manner that extends beyond simple protein-protein association to play an important role in MST1 regulation. PMID: 19962960
    20. RASSF2 is a new member of the RASSF1 family of Ras effectors/tumor suppressors that exhibits a specificity for interacting with K-Ras PMID: 12732644
    21. In colonic neoplsms epigenetic (RASSF2A) and genetic (K-ras) changes are mutually exclusive and provide alternative pathways for affecting Ras signalling. PMID: 15806169
    22. RASSF2 is a novel tumor suppressor gene that regulates Ras signaling and plays a pivotal role in the early stages of colorectal tumorigenesis. PMID: 16012945
    23. RASSF2 is silenced by hypermethylation near the transcription start site in gastric cancer PMID: 16265349
    24. Inactivation of RASSF2A by promoter methylation correlates with lymph node metastasis in nasopharyngeal carcinoma PMID: 17013896
    25. Hypermethylation of the RASSF2A promoter and K-ras/BRAF mutations is associated with microsatellite-stable colorectal cancers PMID: 17013898
    26. Transport of Ras effector superfamily proteins into the nucleus/nucleolus may play a vital role in modulating Ras-mediated cell proliferation during tumorigenesis. PMID: 17320110
    27. Aberrant methylation of the RASSF2 gene with the subsequent loss of RASSF2 expression plays an important role in the pathogenesis of lung cancers. PMID: 17549418
    28. A novel methylation marker for multiple malignancies; suppresses breast tumor cell growth in vitro and in vivo. PMID: 17891178
    29. epigenetic inactivation of RASSF2 plays an important role in oral squamous cell carcinoma tumorigenesis PMID: 18294275
    30. The epigenetic silencing of tumor suppressor genes involved in the Ras/PI3K/AKT pathway plays an important role in oral squamous cell carcinoma radioresistance PMID: 19509163
    31. MST2 and RASSF2 form an active complex in vivo, in which RASSF2 is maintained in a phosphorylated state and protects MST2 from degradation and turnover PMID: 19525978
    32. Data provide evidence that MAPK/ERK-2 mediated phosphorylation regulates nucleo-cytoplasmic transport and cell growth arrest activity of RASSF2. PMID: 19555684
    33. Tumor-specific methylation of APC, MGMT, RASSF2A, and Wif-1 genes might be a valuable biomarker in plasma for the early detection of colorectal cancer. PMID: 19773381

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Chromosome, centromere, kinetochore. Note=Translocates to the cytoplasm in the presence of STK3/MST2 AND STK4/MST1.
  • 组织特异性：
    Widely expressed with highest levels in brain, placenta, peripheral blood and lung. Frequently down-regulated in lung tumor cell lines.
  • 数据库链接：

    HGNC: 9883

    OMIM: 609492

    KEGG: hsa:9770

    STRING: 9606.ENSP00000368684

    UniGene: Hs.631504