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  4. Recombinant Human Rho-related GTP-binding protein RhoB (RHOB)

Recombinant Human Rho-related GTP-binding protein RhoB (RHOB)

  • 中文名称：
    人RHOB重组蛋白
  • 货号：
    CSB-YP019683HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人RHOB重组蛋白
  • 货号：
    CSB-EP019683HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人RHOB重组蛋白
  • 货号：
    CSB-EP019683HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人RHOB重组蛋白
  • 货号：
    CSB-BP019683HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人RHOB重组蛋白
  • 货号：
    CSB-MP019683HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Aplysia RAS-related homolog 6; ARH6; ARHB; H6; MST081; MSTP081; oncogene RHO H6; ras homolog family member B; ras homolog gene family; member B; Rho cDNA clone 6; Rho related GTP binding protein RhoB Precursor; Rho-related GTP-binding protein RhoB; Rhob; RHOB_HUMAN; RHOH6
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-193
  • 氨基酸序列
    MAAIRKKLVV VGDGACGKTC LLIVFSKDEF PEVYVPTVFE NYVADIEVDG KQVELALWDT AGQEDYDRLR PLSYPDTDVI LMCFSVDSPD SLENIPEKWV PEVKHFCPNV PIILVANKKD LRSDEHVRTE LARMKQEPVR TDDGRAMAVR IQAYDYLECS AKTKEGVREV FETATRAALQ KRYGSQNGCI NCC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Required for genotoxic stress-induced cell death in breast cancer cells.
  • 基因功能参考文献：
    1. vesicle-associated RhoB is a regulator of the Rab11-mediated recycling of LFA-1 to the cell surface, an event that is necessary for T lymphocyte motility. PMID: 29233918
    2. These results suggest that miR-223-RhoB signaling pathway plays an important role in modulation of proliferation, cell arrest, and apoptosis in colon cancer. PMID: 29660302
    3. data demonstrate the direct roles of miR-19a and miR-19b on the repression of RhoB and its consequences on tumorigenesis, cancer cell proliferation and invasiveness PMID: 29474434
    4. Our study provides the first evidence that miR-223 can regulate pulmonary artery smooth muscle cells proliferation, migration, and actomyosin reorganization through its novel targets, RhoB and MLC2, resulting in vascular remodeling and the development of pulmonary arterial hypertension. PMID: 27121468
    5. Vgamma9Vdelta2 TCR activation is modulated by the GTPase activity of RhoB and its redistribution to BTN3A1. PMID: 27210746
    6. p53 isoform delta133p53ss regulates cancer cell apoptosis in a RhoB-dependent manner PMID: 28212429
    7. RhoB may play a tumor suppressive role in clear cell renal cell carcinoma cells PMID: 27384222
    8. These results suggest that the levels of RhoG and RhoB GTPases and their negative regulator RhoGDI3 might be linked to the aggressiveness of the pancreatic cancerous cell lines. It is possible that RhoGDI3 could induce the downregulation of RhoG and RhoB. PMID: 27832197
    9. RhoB and ROCK play a key role in preeclampsia. PMID: 27324125
    10. RhoB-specific regulation of Rac1 trafficking controls endothelial barrier integrity during inflammation. PMID: 27138256
    11. The knockdown of RhoB protein decreased the proliferation rate of the parental and the IE1-expressing glioblastoma cells. PMID: 26741994
    12. we propose that RhoB could be responsible for early metastatic prevention by inhibiting the EMT-derived invasiveness of lung cells through the control of PP2A activity. PMID: 26148238
    13. Stimulating its ability to activate the antimetastatic small GTPase RhoB. PMID: 26759237
    14. The isoprenylation and palmitoylation motif of human RhoB (-CINCCKVL) targets chimeric proteins to intraluminal vesicles of endolysosomes in human cells. PMID: 25207810
    15. Findings highlight the degradation of RhoB via the neddylation-CRL pathway as an important molecular event that drives liver carcinogenesis. PMID: 25540389
    16. Data suggest that RhoB belongs to an emerging class of "nononcogene addiction" factors that are essential for maintenance of malignant phenotypes in human cancers. PMID: 25216671
    17. We showed that RhoB is not only a strong prognostic factor in NSCLC but it is also critical for the acquisition of an aggressive phenotype of adenocarcinoma. PMID: 25320360
    18. RhoB depletion reduces cell-cell adhesion and downregulates E-cadherin levels as well as increasing internalized E-cadherin in DU145 prostate cancer cells. PMID: 25630770
    19. Results support previous findings that vaiants in the RHOB and FAM167A-BLK genes may be associated with susceptibility to systemic sclerosis. PMID: 25470816
    20. Suppressing RhoB levels by overexpressing Smurf1 or blocking Chk1-dependent Smurf1 self-degradation significantly inhibits apoptosis. PMID: 25249323
    21. Taken together, our findings offer evidence that in human breast cancer RhoB acts as a positive function to promote expression of ERa and PR in a manner correlated with cell proliferation. PMID: 23339407
    22. RHOB belongs to a novel class of "genes of recurrence" that have a dual role in metastasis and treatment resistance. PMID: 24321314
    23. RhoB expression occurs frequently in ovary carcinogenesis and progression and its expression could be regulated by histone deacetylation but not by promoter hypermethylation. PMID: 24223801
    24. RhoB controls TNFalpha signaling through the regulation of the TNFR traffic. PMID: 24086429
    25. Overexpression of p300, PARP-1, or c-Jun dramatically enhanced RhoB promoter activity. PMID: 24636898
    26. UVB-induced RhoB expression participates in in vivo squamous cell carcinoma initiation by increasing keratinocyte survival. PMID: 23792460
    27. Data indicate that miR-21 induced the degradation of RhoB mRNA, which led to the depletion of RhoB and the impairment of tight junctions in intestinal epithelial cells. PMID: 23583411
    28. RhoB contributes to directional cell migration by regulating beta1 integrin surface levels and activity, thereby stabilizing lamellipodial protrusions. PMID: 22724071
    29. The variants in the RHOB gene were not associated with systemic sclerosis or any of its subsets. PMID: 22896740
    30. Substitution of Val 43 with an Ile in RhoB negatively affected nucleotide exchange in vitro. PMID: 22673745
    31. uPAR rapidly activated RhoB and increased RhoB expression. PMID: 22366462
    32. RhoB may have a key role in ameloblastoma neoplastic epithelial cell phenotype determination, as well as in variant and subtype determination; different results of RhoB are observed in follicular and plexiform ameloblastomas. PMID: 22092654
    33. AE downregulated mRNA expression and promoter/gelatinolytic activity of the RhoB expression at gene and protein level. PMID: 22227305
    34. RhoB mediates adaptational changes to acute hypoxia in the vasculature, but its continual activation by chronic hypoxia can accentuate vascular remodeling to promote development of pulmonary hypertension. PMID: 22539766
    35. miR-21 as a new angiogenesis inhibitor through repression of RhoB PMID: 21347332
    36. Data show that expression of miR-21 in HEK293 and several colorectal cancer cell lines is inversely correlated with ras homolog gene family, member B (RhoB) expression. PMID: 21872591
    37. A mechanism involving the nuclear GEFs Ect2 and Net1 for activating RhoB after genotoxic stress, thereby facilitating cell death after treatment with DNA damaging agents. PMID: 21373644
    38. These results suggest that the activation of p38 MAPK primarily contributes to UV-induced RhoB expression by recruiting the c-Jun and p300 proteins to the distal CCAAT box of the RhoB promoter in Jurkat cells. PMID: 21565167
    39. appears that RhoB plays an opposing role from that of RhoA and/or RhoC in gastric cancer cells. Our work suggests that RhoB may play a tumor suppressor role and subsequently may have potential implications in future targeted therapy PMID: 20473933
    40. Up-regulation of RhoB significantly inhibited heat stress-induced apoptosis and elevated transcriptional activity of NF-kappaB. PMID: 20717930
    41. suppressed RhoB is implicated as a molecular target for therapy against anaplastic thyroid carcinoma cells because RhoB is regulated and growth inhibited. PMID: 20810568
    42. These findings suggest the involvement of RhoB in the process of spermatogenesis in humans PMID: 19997872
    43. miR-21 promotes multiple components of the metastatic phenotype in vitro by regulating several important tumor suppressors, including RHOB. PMID: 20460403
    44. we report, for the first time, the distribution of RhoB protein in the human scalp skin and hair follicles. PMID: 19889053
    45. These results suggest that JNK contributes to RhoB induction, affecting the cell death response to gamma-radiation. PMID: 19995557
    46. RhoB promotes cell motility and invasion in a metastatic prostate cancer cell. PMID: 19782069
    47. Altogether, these results identify a mechanism by which RhoB antagonizes TGF-beta action through transcriptional down-regulation of AP1 in T beta R-II promoter. PMID: 11741970
    48. RhoB is dispensable for normal cell physiology but it has a suppressor or negative modifier function in stress-associated processes including cancer. PMID: 11905808
    49. Histone deacetylase 1 represses the small GTPase RhoB expression in human nonsmall lung carcinoma cell line. PMID: 13679859
    50. RhoB expression is suppressed by EGFR, ErbB2 and Ras, but not Src. PMID: 14647415

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  • 亚细胞定位：
    Late endosome membrane; Lipid-anchor. Cell membrane; Lipid-anchor. Nucleus. Cleavage furrow. Note=Late endosomal membrane (geranylgeranylated form). Plasma membrane (farnesylated form). Also detected at the nuclear margin and in the nucleus. Translocates to the equatorial region before furrow formation in a ECT2-dependent manner.
  • 蛋白家族：
    Small GTPase superfamily, Rho family
  • 数据库链接：

    HGNC: 668

    OMIM: 165370

    KEGG: hsa:388

    STRING: 9606.ENSP00000272233

    UniGene: Hs.502876