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  4. Recombinant Human Rho-related GTP-binding protein RhoD (RHOD)

Recombinant Human Rho-related GTP-binding protein RhoD (RHOD)

  • 货号：
    CSB-YP019688HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP019688HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP019688HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP019688HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    RHOD
  • Uniprot No.：
  • 别名：
    ARHD; Ras homolog D; Ras homolog gene family member A; Ras homolog gene family member D; Rho; RHO D; Rho related GTP binding protein RhoD; Rho related protein HP1; Rho-related GTP-binding protein RhoD; Rho-related protein HP1; RHOD; RHOD_HUMAN; RhoHP1; RHOM
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-207
  • 氨基酸序列
    MTAAQAAGEE APPGVRSVKV VLVGDGGCGK TSLLMVFADG AFPESYTPTV FERYMVNLQV KGKPVHLHIW DTAGQDDYDR LRPLFYPDAS VLLLCFDVTS PNSFDNIFNR WYPEVNHFCK KVPIIVVGCK TDLCKDKSLV NKLRRNGLEP VTYHRGQEMA RSVGAVAYLE CSARLHDNVH AVFQEAAEVA LSSRGRNFWR RITQGFC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in endosome dynamics. May coordinate membrane transport with the function of the cytoskeleton. Involved in the internalization and trafficking of activated tyrosine kinase receptors such as PDGFRB. Participates in the reorganization of actin cytoskeleton; the function seems to involve WHAMM and includes regulation of filopodia formation and actin filament bundling. Can modulate the effect of DAPK3 in reorganization of actin cytoskeleton and focal adhesion dissolution.
  • 基因功能参考文献：
    1. The GTPase deficient atypical Rho GTPases, which have a stalled GTPase activity, RhoD has an elevated intrinsic GDP/GTP exchange activity, rendering the protein constitutively active. PMID: 29776664
    2. RhoD recruits Pak6 to the plasma membrane to antagonize RhoC signaling during cell contraction and blebbing PMID: 28486133
    3. RhoD silencing leads to increased actin filament-containing structures and disruption of cell migration and proliferation. PMID: 28196728
    4. A novel signaling pathway involving RhoD and its binding partner WHAMM, regulate Golgi dynamics. PMID: 25746724
    5. Activated p42/44-MAP kinase, Rho GTPase. PMID: 24706358
    6. Fetal RHD detection in early pregnancy using a single-exon assay in a routine clinical setting is feasible and accurate after its implementation in an unselected pregnant population. PMID: 22776962
    7. It regulates relaxation of vascular smooth muscle. PMID: 24717605
    8. Data from differentiating cultured erythroid precursor cells suggest that RhAG (Rh-associated glycoprotein) knockdown abolishes Rh blood group expression (RhoD; ICAM4 [intercellular adhesion molecule 4]; CD47 Rh-related antigen) in erythroid cells. PMID: 23417980
    9. RhoD interacts with ZIPK in a GTP-dependent manner and modulates stress fiber and focal adhesion reorganization. PMID: 23454120
    10. A GTPase-deficient mutant of RhoD, RhoDG26V, causes hyperplasia and perturbed differentiation of the epidermis. PMID: 22665057
    11. Overexpression of RhoD is associated with multiple myeloma. PMID: 20528248
    12. The expression of RhoA/Rho kinase mRNA and protein and function in the RA were significantly stronger than in the IMA, suggesting that RhoA/Rho kinase pathway may be one mechanism by which RA is more susceptible to spasm than IMA. PMID: 19682162
    13. These results suggest a critical role for the CS amplitude and the balance between Rac and Rho in mechanochemical regulation of lung EC barrier. PMID: 16651639
    14. Methylophiopogonanone B appears to induce Rho activation, resulting in actin cytoskeletal reorganization, including dendrite retraction and stress fiber formation. PMID: 17029007
    15. The data suggest that Rho-kinase dependent cell contractility contributes to global and local matrix remodeling, whereas Rho dependent activation of mDia and/or other downstream effectors regulates the structure and number of cell processes. PMID: 17342762
    16. the the increased expression of p120 isoform 1 during tumor progression contributes to the invasive phenotype of cadherin-deficient carcinomas and that the N-terminal domain of p120 is a valid therapeutic target PMID: 18407999
    17. strongly activated in HTLV-1 infected T cell lines derived from HAM/TSP patients. PMID: 18552504
    18. A previously unknown function of Brk in regulating both RhoA and Ras by phosphorylating p190 and a crucial role of this Brk-elicited signaling pathway in promoting breast malignancy. PMID: 18829532
    19. Rho mediates various phenotypes of malignant transformation by Ras and Src through its effectors, ROCK and mDia [review] PMID: 19160018
    20. Data suggest that mammalian cells have two potential steps that require active Rho for the stabilization of midzone microtubules during mitosis and cytokinesis. PMID: 19576212
    21. estrogen receptor-alpha transcriptional activity is repressed by the Rho/megakaryoblastic leukemia 1 signaling pathway PMID: 19826002

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  • 亚细胞定位：
    Cell membrane; Lipid-anchor; Cytoplasmic side. Early endosome.
  • 蛋白家族：
    Small GTPase superfamily, Rho family
  • 组织特异性：
    Heart, placenta, liver, skeletal muscle, and pancreas and, with weaker intensity, in several other tissues.
  • 数据库链接：

    HGNC: 670

    OMIM: 605781

    KEGG: hsa:29984

    STRING: 9606.ENSP00000308576

    UniGene: Hs.15114