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  4. Recombinant Human Rho-related GTP-binding protein RhoE (RND3)

Recombinant Human Rho-related GTP-binding protein RhoE (RND3)

  • 中文名称：
    人RND3重组蛋白
  • 货号：
    CSB-YP019813HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人RND3重组蛋白
  • 货号：
    CSB-EP019813HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人RND3重组蛋白
  • 货号：
    CSB-EP019813HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人RND3重组蛋白
  • 货号：
    CSB-BP019813HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人RND3重组蛋白
  • 货号：
    CSB-MP019813HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    RND3
  • Uniprot No.：
  • 别名：
    ARHE; memB; Protein MemB; Ras homolog gene family member E; Rho family GTPase 3; Rho-related GTP-binding protein Rho8; Rho-related GTP-binding protein RhoE; Rho8; RhoE; Rnd3; RND3_HUMAN; Small GTP binding protein Rho8
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-241
  • 氨基酸序列
    MKERRASQKL SSKSIMDPNQ NVKCKIVVVG DSQCGKTALL HVFAKDCFPE NYVPTVFENY TASFEIDTQR IELSLWDTSG SPYYDNVRPL SYPDSDAVLI CFDISRPETL DSVLKKWKGE IQEFCPNTKM LLVGCKSDLR TDVSTLVELS NHRQTPVSYD QGANMAKQIG AATYIECSAL QSENSVRDIF HVATLACVNK TNKNVKRNKS QRATKRISHM PSRPELSAVA TDLRKDKAKS C
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins.
  • 基因功能参考文献：
    1. miR200b and miR200c targeted the expression of RhoE and inhibited the malignancy of nonsmall cell lung cancer (NSCLC) cells, and the downregulation of miR200b and miR200c may contribute to the high expression of RhoE in NSCLC. PMID: 30066855
    2. TUG1 can epigenetically inhibit the level of RND3 through binding to EZH2, thus promoting pre-eclampsia development. PMID: 29022920
    3. RND3 promotes Snail 1 protein degradation in glioblastoma tumor cells, promoting cell migration and neoplasm invasiveness. PMID: 27705942
    4. RhoE and ROCK2 regulate chemoresistance in hepatocellular carcinoma. PMID: 27213590
    5. This study Gene-based tests suggest evidence of association with related genes, ZEB2, RND3, MCTP1, CTBP2, and beta EEG. PMID: 28040410
    6. plexin-B2 is a downstream target for Rnd3, which contributes to its cellular function. PMID: 27656111
    7. Studies provide evidence that Rnd3 plays a dual role in cells; on one hand, it regulates cell proliferation, on the other hand, Rnd3 plays an important role in regulating metastasis and invasion. [review] PMID: 27555595
    8. Our data supported that RhoE could inhibit cell proliferation and promote apoptosis. Moreover, these tumor-suppressing effects might be acted through the negative regulation of EGFR/ERK signaling. PMID: 27721256
    9. constant degradation of RND3 by chaperone-mediated autophagy is required to sustain rapid proliferation of gastric cancer cells. PMID: 26761524
    10. We conclude that downregulation of RND3 is responsible for the enhancement of Notch activity that promotes glioblastoma genesis. PMID: 26108681
    11. the development and application of a novel data integration methodology reveals novel functions of RND3 in controlling glioma cell migration, invasion, proliferation, angiogenesis and clinical outcome. PMID: 26132659
    12. Rnd3 regulates lung cancer cell proliferation through notch signaling. PMID: 25372032
    13. CXCR4 might be a downstream effector for RhoE PMID: 24312338
    14. Transfection of pre-miR-200c reduces RhoE expression. PMID: 23821457
    15. RhoE may participate in human cancer progression and act as a candidate target of p53, and these findings also strongly suggest that RhoE may be a new candidate tumor suppressor and could serve as a potential target in the gene therapy of cancer. PMID: 24399089
    16. define RhoE as a Notch1 target that is essential for recruitment of N1IC to promoters of Notch1 target genes, establishing a regulatory feedback loop in Notch1 signaling PMID: 24525741
    17. MicroRNA-200b acts as a stimulant for the proliferation of HTFs by targeting p27/kip1 and RND3. PMID: 24667864
    18. a mechanism according to which proteasomal degradation of RhoE by Skp2 regulates its protein levels to control cellular proliferation. PMID: 24045951
    19. Rnd3 is a regulator of Notch1 signaling PMID: 23630292
    20. Study reports a high-affinity 14-3-3-binding site at the C terminus of Rnd3 consisting of both the Cys241-farnesyl moiety and a Rho-associated coiled coil containing protein kinase (ROCK)-dependent Ser240 phosphorylation site. PMID: 23622247
    21. frequently down-regulated in predominantly HBV-associated hepatocellular carcinomas PMID: 22829315
    22. Loss of Rnd3 expression in keratinocytes results in altered colony morphology. PMID: 22454524
    23. RhoE may play a driving role in the development and progression of ESCC, and targeting the RhoE may be an effective and feasible approach for treatment of ESCC. PMID: 22477709
    24. role of the N-terminal region in signaling; Rnd1 and Rnd3 have a KERRA (Lys-Glu-Arg-Arg-Ala) sequence of amino acids in their N-terminus, which functions as the lipid raft-targeting determinant; the sequence mediates lipid raft targeting of p190 RhoGAP correlated with its activation PMID: 22357615
    25. Rnd3 expression was significantly lower in invasive tumors with satellite nodules PMID: 22234932
    26. Results show that induction of RhoE by cAMP is mediated through protein kinase A (PKA) and promotes BeWo cell fusion but has no effect on functional differentiation. PMID: 22272352
    27. expression of RhoE was significantly negatively associated with serum AFP (P = 0.013) and tumor grade (P = 0.016) PMID: 22213123
    28. The s show that enteropathogenic Escherichia coli translocates EspH, which inactivates mammalian RhoGEFs and triggers cytotoxicity. PMID: 22251971
    29. the expression of miR-17 was negatively correlated with that of RND3 in colorectal cancer tissues and cells PMID: 22132820
    30. This study identifies RhoE as a direct target for HIF-1 in gastric cancer cells. PMID: 22037464
    31. Upregulation of FOXD3 expression following inhibition of B-RAF and MEK correlates with the downregulation of Rnd3, a Rho GTPase and inhibitor of RhoA-ROCK signaling. PMID: 21478267
    32. Data show that the increased invasiveness of tumorigenic cells was associated with reduced expression of Rnd3. PMID: 21209796
    33. RhoE expression in gastric cancer cells was regulated by histone deacetylation, but not by DNA methylation, at the epigenetic level. PMID: 21109974
    34. study demonstrates a novel role for mir-200b in cell cycle progression and identifies RND3 as a novel mir-200b target PMID: 20683643
    35. Socius is a novel Rnd GTPase-interacting protein involved in disassembly of actin stress fibers PMID: 11940653
    36. RhoE is a tumor suppressor gene that is downregulated early in the development of prostate cancer PMID: 15754346
    37. Rnd3/RhoE regulates the assembly of the apical junction complex and tight junction sealing when transfected to a rat tumor cell line. PMID: 15777789
    38. The RND3 mRNA levels increased significantly after gestation in myometria. PMID: 16311049
    39. Study demonstrates that the p53-mediated induction of RhoE in response to DNA damage favors cell survival partly through inhibition of ROCK I-mediated apoptosis. PMID: 17174923
    40. RhoE plays an important role in the regulation of cell proliferation and survival and may be considered as an oncosupressor since it is capable to induce apoptosis in several tumor cell lines. PMID: 17182035
    41. These results identify Rnd3 as a regulator of cross talk between the RAF/MEK/ERK and Rho/ROCK signaling pathways, and a key contributor to oncogene-mediated reorganization of the actin cytoskeleton and focal adhesions. PMID: 18045987
    42. PDK1 competes directly with RhoE for binding to ROCK1. In the absence of PDK1, negative regulation by RhoE predominates, causing reduced acto-myosin contractility and motility. PMID: 18204440
    43. Staphylococcal enterotoxin B-treated renal proximal tubule epithelial cells, show 32 differentially expressed transcripts; one of the down-regulated DETs matched the sequence for Rnd3, which normally inhibits Rho protein function. PMID: 18721871
    44. Data show that RhoE integrates two processes essential for keratinocyte differentiation and stratification: regulation of proliferative status and integrin adhesion. PMID: 18923151
    45. mutation of the RhoE effector region attenuates RhoE-mediated disruption of the actin cytoskeleton, indicating that RhoE exerts its inhibitory effects on ROCK-I through protein(s) binding to its effector region. PMID: 18946488
    46. This study demonstrated that RhoE may promote the multidrug resistance phenotype of gastric cancer cells by decreasing the expression of Bax at posttranscriptional level, thus inhibiting vincristine-induced apoptosis. PMID: 19101510
    47. Findings implicate Rnd3 as a major suppressor of RhoA-mediated actin cytoskeletal organization and in the acquisition of an invasive melanoma phenotype. PMID: 19244113
    48. RhoE inhibits 4E-BP1 phosphorylation and eIF4E function impairing cap-dependent translation. PMID: 19850923

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  • 亚细胞定位：
    Golgi apparatus membrane; Peripheral membrane protein.
  • 蛋白家族：
    Small GTPase superfamily, Rho family
  • 组织特异性：
    Ubiquitous.
  • 数据库链接：

    HGNC: 671

    OMIM: 602924

    KEGG: hsa:390

    STRING: 9606.ENSP00000263895

    UniGene: Hs.6838