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  4. Recombinant Human Ribonucleoside-diphosphate reductase subunit M2 B (RRM2B)

Recombinant Human Ribonucleoside-diphosphate reductase subunit M2 B (RRM2B)

  • 中文名称：
    人RRM2B重组蛋白
  • 货号：
    CSB-EP745339HU
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    DKFZp686M05248; MGC102856; MGC42116; MTDPS8A; MTDPS8B; p53 inducible ribonucleotide reductase small subunit 2 homolog; p53 inducible ribonucleotide reductase small subunit 2 like protein; P53 inducible ribonucleotide reductase small subunit 2 short form beta; p53 R2; p53-inducible ribonucleotide reductase small subunit 2-like protein; p53R2; Ribonucleoside diphosphate reductase M2 subunit B; Ribonucleoside-diphosphate reductase subunit M2 B; Ribonucleotide reductase M2 B (TP53 inducible); Ribonucleotide reductase M2 B; Ribonucleotide reductase small subunit like 2 p53 inducible; RIR2B_HUMAN; RRM 2B; RRM2B; TP53 inducible ribonucleotide reductase M2 B; TP53-inducible ribonucleotide reductase M2 B
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    67.7kDa
  • 表达区域：
    1-351aa
  • 氨基酸序列
    MGDPERPEAAGLDQDERSSSDTNESEIKSNEEPLLRKSSRRFVIFPIQYPDIWKMYKQAQASFWTAEEVDLSKDLPHWNKLKADEKYFISHILAFFAASDGIVNENLVERFSQEVQVPEARCFYGFQILIENVHSEMYSLLIDTYIRDPKKREFLFNAIETMPYVKKKADWALRWIADRKSTFGERVVAFAAVEGVFFSGSFAAIFWLKKRGLMPGLTFSNELISRDEGLHCDFACLMFQYLVNKPSEERVREIIVDAVKIEQEFLTEALPVGLIGMNCILMKQYIEFVADRLLVELGFSKVFQAENPFDFMENISLEGKTNFFEKRVSEYQRFAVMAETTDNVFTLDADF
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal GST-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. Supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. Contains an iron-tyrosyl free radical center required for catalysis. Forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage.
  • 基因功能参考文献：
    1. The expression of p53R2 was associated closely with the development and progression of lung sarcomatoid carcinoma. PMID: 29246119
    2. p53R2 protein is overexpressed in early-stage cervical cancer and unravels some unconventional oncogenic functions of p53R2. PMID: 28841361
    3. High RRM2B expression is associated with neoplasms. PMID: 27004405
    4. Studied the expression of ribonucleotide reductase (RR) subunit p53R2 in nasopharyngeal carcinoma and its significance in the prognosis. PMID: 29237424
    5. RRM1/RRM2B enzyme is capable of retaining activity in hypoxia and therefore is favored over RRM1/RRM2 in order to preserve ongoing replication and avoid the accumulation of DNA damage in hypoxic cells. PMID: 28416140
    6. A newly discovered role of E2F1 in the regulation of p53R2 expression in DNA damage response. PMID: 25312903
    7. analysis of caspase-dependent degradation of human R2 and p53R2 small subunits PMID: 25878246
    8. in Turkish population p53R2 genotype distributions between head and neck squamous epithelial cell cancer patients and control groups were not statistically significantly different. PMID: 24861915
    9. Data indicate that forkhead transcription factorsF OXO3 directly bound to and transcriptionally activated the promoter of ribonucleotide reductase subunit RRM2B, and induced the expression of RRM2B at RNA and protein levels. PMID: 24947616
    10. we found no support of the hypothesis that aberrations of RRM1 or RRM2B, neither individually nor in combination, are associated with an altered clinical outcome following chemotherapy. PMID: 24215511
    11. Ribonucleotide reductase M2B inhibits cell migration and spreading by early growth response protein 1-mediated phosphatase and tensin homolog/Akt1 pathway in hepatocellular carcinoma. PMID: 24214128
    12. RRM2B expression may discriminate cervical cancer phenotype and radiochemotherapy outcome PMID: 23552804
    13. RRM2B is highly induced in a p53-dependent manner during senescence and is expressed at higher levels in senescent precancerous human prostatic intraepithelial neoplasm lesions compared to adjacent normal prostate glands. PMID: 23139867
    14. p53R2 could regulate matrix synthesis via Akt phosphorylation during chondrocyte mechanotransduction. Down-regulation of p53R2 may be a new therapeutic approach in OA therapy. PMID: 22954457
    15. p53R2 is directly regulated by p53 and also by a MEK2 (ERK kinase 2/MAP kinase kinase 2)-dependent pathway. PMID: 22895183
    16. Propose p53R2 as a therapeutic target to enhance the effectiveness of chemotherapy in patients with p53R2-positive melanoma. PMID: 22902076
    17. These results confirm a role for p53R2 in both Clofarabine and decitabine mechanism of action PMID: 22884950
    18. In nontransformed cells only during quiescence, protein p53R2 is required for maintenance of mitochondrial DNA and for optimal DNA repair after ultraviolet damage. PMID: 22847445
    19. Evidence that disease pathogenesis may be caused by defective RNR assembly is given. PMID: 21378381
    20. Increased expression of p53R2 may predict gemcitabine resistance, and upregulated RNR activity may influence gemcitabine resistance in cholangiocarcinoma cells. PMID: 21451941
    21. p53R2 may suppress cancer cell proliferation; plays critical roles not only in DNA damage repair but also in proliferation of cancer cells PMID: 21216934
    22. p53R2 expression seems more important than that of hRRM2 in prognosis of early-stage lung cancer. PMID: 21965764
    23. this study provides functional evidence that mitochondria is one of p53R2-targeted organelles and suggests an unexpected function of p53R2, which is beyond known ribonucleotide reductase function on dNTP synthesis, in mitochondrial homeostatic control. PMID: 21640705
    24. Adult-onset progressive external ophthalmoplegia due to RRM2B mutations is associated with a benign myopathic phenotype and characterized by muscle-restricted, mitochondrial DNA deletions. PMID: 21646632
    25. oss of p53R2 affects ribonucleotide reduction only in resting cells and leads to a decrease of dNTP catabolism by substrate cycles that counterweigh the loss of anabolic activity PMID: 21297166
    26. This study correlates the distinct catalytic mechanisms of the small subunits hp53R2 and hRRM2 with a hydrogen-bonding network. PMID: 20484015
    27. p53R2-dependent DNA synthesis plays a pivotal role in cell survival by repairing damaged DNA PMID: 11719458
    28. Expression of p53R2, newly p53 target in oral normal epithelium, epithelial dysplasia and squamous cell carcinoma. PMID: 12565178
    29. Wild-type p53 regulates human ribonucleotide reductase by protein-protein interaction with p53R2 as well as hRRM2 subunits. PMID: 12615712
    30. UV-induced activation of p53R2 transcription and binding of p53R2 to hRRM1 to form RR holoenzyme are impaired in the p53-mutant cell line PC3. PMID: 14583450
    31. Four regulatory-region variants were found, of which three were single nucleotide polymorphisms (SNPs) (nt 1 789 C/G, nt 1 928 A/G, 1 933 T/C), and one was 20 bp insertion which replaced a ATTTT between nt 1831 and 1835. PMID: 16127747
    32. we developed a p53R2-dependent luciferase reporter gene assay, and demonstrated dose-dependent luminescence caused by adriamycin in two human cell lines that express wild-type p53, MCF-7 and HepG2 PMID: 16236544
    33. RRM2 and p53R2 subunits share the same binding site on RRM1 PMID: 16376858
    34. Expressions of p53 and p53R2 proteins should be useful for determining the tumor properties, including prognosis, in patients with esophageal squamous cell carcinoma. PMID: 16778101
    35. Catalytic properties are assessed. PMID: 16846634
    36. ectopic expression of human papillomavirus 16 E6 impaired p53R2 induction after DNA damage in human fibroblasts PMID: 16872707
    37. Mutation of RRM2B is associated with mitochondrial Diseases PMID: 17486094
    38. p53R2 expression is reduced after X-ray irradiation following the transfection of p53R2 siRNAin esophageal squamous cell carcinoma. This could potentially improve efficacy of radiotheraepy. PMID: 17671702
    39. a protective function of the p53R2 ribonucleotide reductase subunit in prevention or repair of NO-mediated genotoxic injury. PMID: 18474260
    40. Sequencing the RRM2B gene revealed three missense mutations and two single nucleotide deletions in exons 6, 8, and 9, confirming that RRM2B mutations are important causes of MDS. PMID: 18504129
    41. analysis of the effects of p53R2 silencing on DNA damage in LNCaP cells PMID: 18505925
    42. p53R2 constitutes a potential target for anticancer drugs as well as a diagnostic marker in cancer. PMID: 18760875
    43. The redox property, structure, and function of hRRM2 and p53R2, are studied. PMID: 19082948
    44. This report describes two brothers, both deceased in infancy, with severe depletion of mitochondrial DNA (mtDNA) in muscle tissue. A novel missense mutation in the RRM2B gene, encoding the p53R2 subunit, was identified PMID: 19138848
    45. A heterozygous truncating mutation in RRM2B causes autosomal-dominant progressive external ophthalmoplegia with multiple mtDNA deletions. PMID: 19664747
    46. This study expands the clinical spectrum of impaired RIR2B function. PMID: 19667227
    47. The X-ray crystal structure of human p53R2 is determined to 2.6 A, in which monomers A and B exhibit mono- and binuclear iron occupancy, respectively. Sequence-structure-function correlations that differentiate p53R2 and RRM2 are revealed. PMID: 19728742

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  • 相关疾病：
    Mitochondrial DNA depletion syndrome 8A (MTDPS8A); Mitochondrial DNA depletion syndrome 8B (MTDPS8B); Progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal dominant, 5 (PEOA5)
  • 亚细胞定位：
    Cytoplasm. Nucleus. Note=Translocates from cytoplasm to nucleus in response to DNA damage.
  • 蛋白家族：
    Ribonucleoside diphosphate reductase small chain family
  • 组织特异性：
    Widely expressed at a high level in skeletal muscle and at a weak level in thymus. Expressed in epithelial dysplasias and squamous cell carcinoma.
  • 数据库链接：

    HGNC: 17296

    OMIM: 604712

    KEGG: hsa:50484

    STRING: 9606.ENSP00000251810

    UniGene: Hs.512592