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  4. Recombinant Human SAM pointed domain-containing Ets transcription factor (SPDEF)

Recombinant Human SAM pointed domain-containing Ets transcription factor (SPDEF)

  • 货号：
    CSB-YP022518HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP022518HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP022518HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP022518HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP022518HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SPDEF
  • Uniprot No.：
  • 别名：
    bA375E1.3; PDEF; Prostate derived Ets factor; PROSTATE EPITHELIUM SPECIFIC ETS TRANSCRIPTION FACTOR; Prostate epithelium-specific Ets transcription factor; Prostate specific Ets; Prostate-derived Ets factor; Prostate-specific Ets; PSE; RP11-375E1__A.3; SAM POINTED DOMAIN CONTAINING ETS TRANSCRIPTION FACTOR; SAM pointed domain-containing Ets transcription factor; Spdef; SPDEF_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-335
  • 氨基酸序列
    MGSASPGLSS VSPSHLLLPP DTVSRTGLEK AAAGAVGLER RDWSPSPPAT PEQGLSAFYL SYFDMLYPED SSWAAKAPGA SSREEPPEEP EQCPVIDSQA PAGSLDLVPG GLTLEEHSLE QVQSMVVGEV LKDIETACKL LNITADPMDW SPSNVQKWLL WTEHQYRLPP MGKAFQELAG KELCAMSEEQ FRQRSPLGGD VLHAHLDIWK SAAWMKERTS PGAIHYCAST SEESWTDSEV DSSCSGQPIH LWQFLKELLL KPHSYGRFIR WLNKEKGIFK IEDSAQVARL WGIRKNRPAM NYDKLSRSIR QYYKKGIIRK PDISQRLVYQ FVHPI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    May function as an androgen-independent transactivator of the prostate-specific antigen (PSA) promoter. Binds to 5'-GGAT-3' DNA sequences. May play a role in the regulation of the prostate gland and/or prostate cancer development. Acts as a transcriptional activator for SERPINB5 promoter.
  • 基因功能参考文献：
    1. PSMA, TERT, and PDEF may serve as a reference for clinical diagnosis and as potential targets for the malignant tumor of the prostate therapeutics PMID: 28829509
    2. This is, to our knowledge, the first report of decreased expression of TFF3, SPDEF, KLF4, and goblet cell population in the colon of patients with HSCR. Altered goblet cell function may result in intestinal barrier dysfunction contributing to the development of HAEC. PMID: 29383490
    3. Loss of SPDEF promotes prostate cancer growth and metastasis and can be caused by dysregulation or therapeutic inhibition of androgen receptor signaling. PMID: 28811384
    4. Results demonstrated that SPDEF expression is increased during IL-13-induced goblet cell differentiation, which correlates to hypermethylation of CpG number 8 in the SPDEF promoter. These findings suggest that aberrant DNA methylation of SPDEF is one of the factors underlying mucus hypersecretion in COPD. PMID: 28450970
    5. Taken together, these results suggested a novel function for SPDEF wherein it represses canonical Wnt/b-catenin transcriptional activity through selectively displacing b-catenin from chromatin targets. PMID: 28390865
    6. these findings demonstrate PDEF to be an oncogenic driver of breast cancer and a biomarker of poor prognosis in this cancer. Based on this understanding and the limited expression of PDEF in normal human tissues, the development of PDEF-based therapeutics for prevention and treatment of breast cancer is also discussed PMID: 28468594
    7. These results indicate efficient SPDEF silencing and downregulation of mucus-related gene expression by epigenetic editing, in human lung epithelial cells. PMID: 28011616
    8. miR-125b inhibits SPDEF expression modulating goblet cell differentiation and mucus secretion in asthma PMID: 27112664
    9. PDEF regulates many genes, which participate in various stages of tumor development. PMID: 27612480
    10. PDEF gene expression is associated with the extent of bladder neoplasia and PDEF modulated the expressions of EMT-related genes. PMID: 26965996
    11. SPDEF inhibits prostate carcinogenesis by disrupting a positive feedback loop in regulation of the Foxm1 oncogene. PMID: 25254494
    12. We found significantly increased SPDEF and MUC5AC expression in CRSwNP patients compared to the controls (P < .05); the mRNA level of SPDEF was positively correlated with MUC5AC expression in CRS patients (P < .05). PMID: 25376946
    13. we identified four proteins with different expression in paclitaxel resistant cells, i.e., serpin B3, serpin B4, heat shock protein 27 (all three upregulated) and cytokeratin 18 (downregulated). PMID: 24898171
    14. PDEF undergoes DNA methylation in breast cancer cells. PMID: 23921628
    15. Host Kruppel-like factor 15, Slug, and SPDEF, stimulated the herpes simplex virus type 1 ICP0 promoter more than 150-fold. PMID: 24027338
    16. Data indicate that Il-9 and IL-13 regulate expressions of MUC5AC, SPDEF and matrix metalloproteinase 7 (MMP-7) in pediatric bronchial epithelial cell. PMID: 23671562
    17. these findings show that PDEF-CEACAM6 is a highly active oncogenic axis in breast cancer PMID: 23592399
    18. Data demonstrate that SPDEF is required for conjunctival goblet cell differentiation and down-regulation of SPDEF may play a role in human dry eye with goblet cell loss. PMID: 23665202
    19. Prostate-derived ETS factor (PDEF) is identified as a mediator of mammary luminal epithelial lineage-specific gene expression and as a factor required for tumorigenesis in a subset of breast cancers. PMID: 23764000
    20. In colorectal tumors from patients, loss of SPDEF was observed in approximately 85% of tumors and correlated with progression from normal tissue, to adenoma, to adenocarcinoma. PMID: 23376423
    21. Data show that SAM pointed domain ETS factor (SPDEF) was induced by rhinoviral infection of primary human airway cells. PMID: 23012424
    22. SPDEF functions as a tumor metastasis suppressor in vivo. PMID: 22761428
    23. PDEF may inhibit prostate cancer progression by transcriptional downregulation of oncogenic STMN expression. PMID: 22378487
    24. PDEF is a negative regulator of prostate tumor progression and the miR-204-PDEF regulatory axis contributes to PDEF protein loss and resultant cancer progression. PMID: 21446014
    25. Studies indicate that PDEF is a negative regulator of tumor progression and metastasis. PMID: 21764212
    26. Up-regulation of PDEF is associated with a favorable prognosis in patients with clinically localized prostate cancer. PMID: 21656828
    27. results show that SPDEF plays a critical role in regulating a transcriptional network mediating IL-13-induced MUC5AC synthesis dependent on STAT6 PMID: 21275604
    28. studies demonstrate for the first time negative regulation of MMP9 expression by PDEF, and that PDEF expression was lost in aggressive prostate cancer and was inversely associated with MMP9 expression in clinical samples of prostate cancer PMID: 20550708
    29. Transcriptional regulation of p21/CIP1 cell cycle inhibitor by PDEF controls cell proliferation and mammary tumor progression PMID: 20139077
    30. PDEF is a negative regulator of colon cancer cell growth and migration. PMID: 19830706
    31. Spdef was sufficient to inhibit proliferation of intestinal progenitors and induce differentiation into goblet cells. PMID: 19786015
    32. NKX-3.1 interacts with prostate-derived Ets factor and regulates the activity of the PSA promoter. PMID: 11809674
    33. PDEF upregulates p62 promoter activity at 2 sites. PSI downregulates PDEF-induced p62 promoter activation through one of these sites. PMID: 12700667
    34. Pdef loss may alter the expression of genes controlling progression to invasive breast cancer. PMID: 12907642
    35. identified novel protein-protein interaction domains within NKX-3.1 and PDEF that operate in concert with their respective DNA binding domains to mediate functional interactions between these growth regulatory transcription factors PMID: 15523673
    36. Despite common essential features that are shared among Ets members, the crystal structure of PDEF Ets domain demonstrates distinct patterns of interactions at different positions of DNA in achieving sequence specific recognition. PMID: 15882048
    37. RUNX factors participate in prostate epithelial cell function and cooperate with an Ets transcription factor to regulate PSA gene expression PMID: 16237704
    38. PDEF overexpression is associated with activation of ErbB2 and CSF-1R and breast tumor progression PMID: 16357167
    39. prostate-derived Ets factor is a potential marker and target in ovarian cancer PMID: 17197890
    40. SPDEF was induced following intratracheal allergen exposure and after Th2 cytokine stimulation and was sufficient to cause goblet cell differentiation of Clara cells in vivo. PMID: 17347682
    41. these results for the first time show frequent increased expression of PDEF protein in breast and prostate tumors and support a role for PDEF in breast and prostate cancer progression. PMID: 17521701
    42. PDEF mRNA expression could be useful in breast cancer molecular staging. PMID: 17971898
    43. increased PDEF expression along with reduced survivin was associated with prolonged survival of patients with ovarian cancer PMID: 18567002
    44. analysis of PDEF transcriptional networks regulating cell migration during cancer progression PMID: 18579687
    45. prostate derived Ets factor mRNA quantitation did not reveal any significant differences between breast cancer patients and healthy women PMID: 18846421
    46. PDEF may be regulated by Erbb2 or EGFR-activated signaling pathways in breast cancer cells. PMID: 19203193
    47. we hypothesize that SPDEF has a function in the specification of the progenitor cells of the luminal epithelial lineage that become targets of oncogenesis in luminal breast cancer. PMID: 19505923

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    ETS family
  • 组织特异性：
    Expressed in a very restricted set of primarily hormone-regulated epithelial tissues with particularly high expression in the prostate gland. Significantly lower expression is seen in other hormone regulated tissues such as mammary gland, salivary gland,
  • 数据库链接：

    HGNC: 17257

    OMIM: 608144

    KEGG: hsa:25803

    STRING: 9606.ENSP00000363149

    UniGene: Hs.485158