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  4. Recombinant Human STAM-binding protein (STAMBP)

Recombinant Human STAM-binding protein (STAMBP)

  • 货号：
    CSB-YP022794HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP022794HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP022794HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP022794HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP022794HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    AMSH; Associated molecule with the SH3 domain of STAM; Endosome associated ubiquitin isopeptidase; Endosome-associated ubiquitin isopeptidase; MGC126516; MGC126518; MICCAP; STABP_HUMAN; STAM binding protein; STAM-binding protein; Stambp
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-424
  • 氨基酸序列
    MSDHGDVSLP PEDRVRALSQ LGSAVEVNED IPPRRYFRSG VEIIRMASIY SEEGNIEHAF ILYNKYITLF IEKLPKHRDY KSAVIPEKKD TVKKLKEIAF PKAEELKAEL LKRYTKEYTE YNEEKKKEAE ELARNMAIQQ ELEKEKQRVA QQKQQQLEQE QFHAFEEMIR NQELEKERLK IVQEFGKVDP GLGGPLVPDL EKPSLDVFPT LTVSSIQPSD CHTTVRPAKP PVVDRSLKPG ALSNSESIPT IDGLRHVVVP GRLCPQFLQL ASANTARGVE TCGILCGKLM RNEFTITHVL IPKQSAGSDY CNTENEEELF LIQDQQGLIT LGWIHTHPTQ TAFLSSVDLH THCSYQMMLP ESVAIVCSPK FQETGFFKLT DHGLEEISSC RQKGFHPHSK DPPLFCSCSH VTVVDRAVTI TDLR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Zinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not cleave 'Lys-48'-linked polyubiquitin chains. Plays a role in signal transduction for cell growth and MYC induction mediated by IL-2 and GM-CSF. Potentiates BMP (bone morphogenetic protein) signaling by antagonizing the inhibitory action of SMAD6 and SMAD7. Has a key role in regulation of cell surface receptor-mediated endocytosis and ubiquitin-dependent sorting of receptors to lysosomes. Endosomal localization of STAMBP is required for efficient EGFR degradation but not for its internalization. Involved in the negative regulation of PI3K-AKT-mTOR and RAS-MAP signaling pathways.
  • 基因功能参考文献：
    1. Contrary to previously reported STAMBP mutations, the Ser236Phe mutation did not lead to constitutive activation of the PI3K-AKT-mTOR pathway in patient-derived LCLs, as indicated by the expression of phosphorylated S6 ribosomal protein, suggesting that it is not the major pathomechanism underlying the disorder in this patient. PMID: 29907875
    2. Targeting the deubiquitinase STAMBP inhibits NALP7 inflammasome activity. PMID: 28492230
    3. Two siblings with classic features of MIC-CAP syndrome that harbor a novel predicted splice mutation in STAMBP PMID: 25692795
    4. associated molecule with the SH3 domain of STAM-mediated deubiquitination of connexin 43 protects Gap junctions from degradation PMID: 25070368
    5. Recognition of the proximal ubiquitin is imperative for the linkage specificity and catalytic efficiency of AMSH. PMID: 24151880
    6. Mutations in STAMBP, encoding a deubiquitinating enzyme, cause microcephaly-capillary malformation syndrome. PMID: 23542699
    7. AMSH efficiently removes ubiquitin from the activated EGFR. PMID: 22800866
    8. tight coupling of ESCRT-III CHMP3 and AMSH functions and provide insight into the regulation of ESCRT-III PMID: 21827950
    9. Studies indicate that USP8/Ubpy and AMSH interact with ESCRT components to modulate the ubiquitination status of receptors and relevant sorting proteins. PMID: 21448666
    10. AMSH interacts with ESCRT-0 to regulate the stability and trafficking of CXCR4. PMID: 20159979
    11. AMSH is a deubiquitinating enzyme with functions at the endosome to oppose ubiquitin-dependent receptor sorting PMID: 15314065
    12. The cellular functions of UBPY are complex but clearly distinct from those of the Lys-63-ubiquitin-specific protease, AMSH, with which it shares a binding site on the SH3 domain of STAM PMID: 16520378
    13. AMSH and AMSH-LP are anchored on the early endosomal membrane via interaction with the clathrin coat PMID: 16716190
    14. AMSH has a role in the deubiquitination of the endosomal cargo preceding lysosomal degradation PMID: 16760479
    15. AMSH, which has been recently characterized as a regulator of the endosomal sorting of epidermal growth factor receptor, represents a novel modulator of CaR signaling. PMID: 16854379
    16. both the deubiquitination enzyme activity of AMSH and its CHMP3-binding ability are required to clear ubiquitinated cargo from endosomes PMID: 17159328
    17. Endosomal AMSH (STAMBP) is a functional component of the multivesicular body pathway. PMID: 17261583
    18. UBPY MIT domain and another ubiquitin isopeptidase, AMSH, reveals common interactions with CHMP1A and CHMP1B but a distinct selectivity of AMSH for CHMP3/VPS24, a core subunit of the ESCRT-III complex, and UBPY for CHMP7. PMID: 17711858
    19. crystal structures of the human AMSH-LP DUB domain alone and in complex with a Lys 63-linked di-ubiquitin at 1.2 A and 1.6 A resolutions, respectively PMID: 18758443
    20. Data suggest that AMSH and UBPY are essential for trafficking and down-regulation of PAR(2) but not for regulating PAR(2) dissociation from beta-arrestin2 or PAR(2)-mediated ERK2 activation. PMID: 19684015

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  • 相关疾病：
    Microcephaly-capillary malformation syndrome (MICCAP)
  • 亚细胞定位：
    Nucleus. Membrane; Peripheral membrane protein. Cytoplasm. Early endosome.
  • 蛋白家族：
    Peptidase M67C family
  • 组织特异性：
    Ubiquitously expressed.
  • 数据库链接：

    HGNC: 16950

    OMIM: 606247

    KEGG: hsa:10617

    STRING: 9606.ENSP00000344742

    UniGene: Hs.469018