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  4. Recombinant Human Segment polarity protein dishevelled homolog DVL-2 (DVL2)

Recombinant Human Segment polarity protein dishevelled homolog DVL-2 (DVL2)

  • 货号：
    CSB-YP007286HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP007286HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP007286HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP007286HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP007286HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Dishevelled 2 (homologous to Drosophila dsh); Dishevelled dsh homolog 2; dishevelled segment polarity protein 2; Dishevelled-2; Dishevelled2; DSH homolog 2; DVL 2; Dvl2; DVL2_HUMAN; Segment polarity protein dishevelled homolog DVL 2; Segment polarity protein dishevelled homolog DVL-2; Segment polarity protein dishevelled homolog DVL2
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-736
  • 氨基酸序列
    MAGSSTGGGG VGETKVIYHL DEEETPYLVK IPVPAERITL GDFKSVLQRP AGAKYFFKSM DQDFGVVKEE ISDDNARLPC FNGRVVSWLV SSDNPQPEMA PPVHEPRAEL APPAPPLPPL PPERTSGIGD SRPPSFHPNV SSSHENLEPE TETESVVSLR RERPRRRDSS EHGAGGHRTG GPSRLERHLA GYESSSTLMT SELESTSLGD SDEEDTMSRF SSSTEQSSAS RLLKRHRRRR KQRPPRLERT SSFSSVTDST MSLNIITVTL NMEKYNFLGI SIVGQSNERG DGGIYIGSIM KGGAVAADGR IEPGDMLLQV NDMNFENMSN DDAVRVLRDI VHKPGPIVLT VAKCWDPSPQ AYFTLPRNEP IQPIDPAAWV SHSAALTGTF PAYPGSSSMS TITSGSSLPD GCEGRGLSVH TDMASVTKAM AAPESGLEVR DRMWLKITIP NAFLGSDVVD WLYHHVEGFP ERREARKYAS GLLKAGLIRH TVNKITFSEQ CYYVFGDLSG GCESYLVNLS LNDNDGSSGA SDQDTLAPLP GATPWPLLPT FSYQYPAPHP YSPQPPPYHE LSSYTYGGGS ASSQHSEGSR SSGSTRSDGG AGRTGRPEER APESKSGSGS ESEPSSRGGS LRRGGEASGT SDGGPPPSRG STGGAPNLRA HPGLHPYGPP PGMALPYNPM MVVMMPPPPP PVPPAVQPPG APPVRDLGSV PPELTASRQS FHMAMGNPSE FFVDVM
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a role in the signal transduction pathways mediated by multiple Wnt genes. Participates both in canonical and non-canonical Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Promotes internalization and degradation of frizzled proteins upon Wnt signaling.
  • 基因功能参考文献：
    1. Results show that WIF1-mediated autophagy inhibits Wnt/betacatenin signaling by downregulating Dvl2, which contributes to the inhibition of the proliferation and the promotion of the apoptosis of nonsmall cell lung cancer cells. PMID: 29916529
    2. The inactivation of Wnt3a reversed the effect of Dvl2 silencing. In conclusion, the results indicate that Dvl2 regulated osteogenic differentiation of SFBs in osteoarthritis. PMID: 29749449
    3. Overexpression of dishevelled 2 is associated with metastasis in hepatocellular carcinoma. PMID: 28589433
    4. FZD4 Tyrosine 250(2.39) is crucial for DVL2 interaction and DVL2 translocation to the plasma membrane. PMID: 28668722
    5. we found that Wnt3a treatment rapidly induces hyperphosphorylation and stabilization of Dvl2 and Dvl3. Our findings suggest a model of positive regulation of PKCzeta-mediated Dvl signaling activity, to produce a strong and sustained response to Wnt3a treatment by stabilizing Dvl protein levels. PMID: 28366812
    6. Dvl2 could play a part in epithelial ovarian cancer progression and might be an independent prognostic factor. Additionally, it might be a prospective therapeutic target in the treatment of EOC. PMID: 28513833
    7. Our findings reveal an underappreciated role of Dvl2 in regulating inflammation and rheumatoid arthritis-fibroblast-like synoviocyte apoptosis PMID: 28187436
    8. inhibition of DVL2 can sensitize cisplatin-resistant lung cancer cells through down-regulating Wnt/beta-catenin signaling. PMID: 27432651
    9. ESCC patients with high Dvl2 expression had worse prognoses and multivariate analysis indicated that Dvl2 was an independent risk factor for ESCC patients' survival. Our clinical data indicated that Dvl2 contribute to the malignant progression of ESCC and might be a prognostic biomarker. PMID: 27083564
    10. In pancreatic cancer cells, AF6 is expressed at reduced levels, causing Dvl2 to be upregulated and available to bind and enhance FOXE1-induced trans-activation of Snail, which promotes proliferation and metastasis. PMID: 26013125
    11. Epsins are required for Dishevelled stability and Wnt signalling activation in colon cancer development. PMID: 25871009
    12. The data suggest that the expression of DVL2 in colon tissue segments may be important in the pathogenesis of HSCR. PMID: 25395054
    13. Elevated DVL2 expression is associated with drug resistance in colorectal cancer. PMID: 24893630
    14. Dpr1 promotes the ubiquitination of Dvl2 by pVHL and mediates the protein aggregate-elicited autophagy initiation PMID: 25825496
    15. The interaction of Dvl2 with Dapper-1 is involved in the negative regulation of Wnt signaling. PMID: 25558878
    16. APPL1 is a positive regulator of Dvl2-dependent transcriptional activity of AP-1. PMID: 25622892
    17. High expression of IGFBP7 in fibroblasts induced by colorectal cancer cells is co-regulated by TGF-beta and Wnt signaling in a Smad2/3-Dvl2/3-dependent manner. PMID: 24427302
    18. these results indicate that RNF185 negatively regulates osteogenesis through the degradation of Dvl2 and down-regulation of canonical Wnt signaling pathway and suggest a possible therapeutic target in osteoporosis. PMID: 24727453
    19. IRS1/2 promotes EMT and cell proliferation through stabilizing Dvl2. PMID: 24616100
    20. Dvl2 knockdown in LNCaP cells reduces malignant cell behavior by suppressing Wnt-3a signaling and MMP activity. PMID: 23652996
    21. In a cohort of patients with neural tube defects but not in controls, 5 rare mutations were found.p.Tyr667Cys & p.Ala53Val were predicted to be harmful. A patient with caudal agenesis had a 1-bp insertion (c.1801_1802insG) in exon 15. PMID: 22892949
    22. Both single-marker and haplotype analyses showed an association between SNPs in the DVL2 gene and the risk for cleft lip with or without cleft palate. PMID: 22887353
    23. Wnt-induced dishevelled 2 phosphorylation has effects in in canonical and noncanonical Wnt3a and Wnt5a signaling PMID: 23396967
    24. site-specific Dvl2 phosphorylation is required for Dvl2 association with PKCiota; this interaction is likely to be one of the mechanisms essential for Wnt3a-dependent neurite outgrowth PMID: 23396968
    25. NEDD4L is able to directly bind Dvl2 and target Dvl2 for proteasomal degradation. PMID: 23396981
    26. Dvl2 protein transduce signals via the Wnt proteins non canonical pathways, namely via NFAT protein and Src kinase and novel NPM-ALK interacting proteins and possibly NPM-ALK substrates. PMID: 23022960
    27. RIPK4 interacted constitutively with the adaptor protein DVL2; phosphorylation of DVL2 by RIPK4 favored canonical Wnt signaling PMID: 23371553
    28. Overexpression of ITCH inhibited wild-type DVL2 -induced, but not DVL2-Y568F mutant-induced, Wnt reporter activity. PMID: 22826439
    29. Wnt5a promotes breast cancer cell migration via Dvl2/Daam1/RhoA. PMID: 22655072
    30. up-regulation of DVL2 is associated with androgen-independent prostate carcinoma PMID: 21487968
    31. Our results indicate that malin regulates Wnt signaling pathway through the degradation of dishevelled2 and suggest possible deregulation of Wnt signaling in Lafora disease. PMID: 22223637
    32. Dishevelled 2 (Dvl2), a key component of the Wnt signaling pathway, is overexpressed in human gliomas. PMID: 21990322
    33. These findings identify a role for beta-arrestin and Dvl-2 scaffolds in APC-activated PAR1 cytoprotective signaling in human endothelial cells. PMID: 22106258
    34. identification of a leucine-rich binding motif strongly resembling the consensus sequence of a nuclear export signal in human Dishevelled-2 using C-terminal phage display PMID: 21666888
    35. A novel function of Dishevelled protein in modulating NF-kappaB-regulated gene transcription. PMID: 20628365
    36. These results suggested that Dvl2 is involved in mitotic progression by regulating the dynamics of MT plus-ends and the SAC in Plk1-dependent and -independent manners. PMID: 20823832
    37. Binding of Dvl2 to p62 facilitates the aggregation and the LC3-mediated autophagosome recruitment of Dvl2 under starvation; the ubiquitylated Dvl2 aggregates are ultimately degraded through the autophagy-lysosome pathway. PMID: 20639871
    38. DACT1 antagonizes Wnt signaling by promoting DVL2 degradation. PMID: 16446366
    39. The DIX domain of Dishevelled confers Wnt signaling by dynamic polymerization. PMID: 17529994
    40. These data provide evidence that there is a novel signaling pathway from Dishevelled to p53. PMID: 17593335
    41. BAMBI interacts with Wnt receptor Frizzled5, coreceptor LRP6, and Dishevelled2 and increases the interaction between Frizzled5 and Dishevelled2 PMID: 18838381

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  • 亚细胞定位：
    Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytosol. Cytoplasmic vesicle. Nucleus.
  • 蛋白家族：
    DSH family
  • 数据库链接：

    HGNC: 3086

    OMIM: 602151

    KEGG: hsa:1856

    STRING: 9606.ENSP00000005340

    UniGene: Hs.118640