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  4. Recombinant Human Septin-9 (41526)

Recombinant Human Septin-9 (41526)

  • 货号：
    CSB-YP883392HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP883392HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP883392HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP883392HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP883392HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SEPT9
  • Uniprot No.：
  • 别名：
    AF17q25; Cell division control protein septin D1; KIAA0991; MLL septin like fusion protein; MLL septin-like fusion protein; MLL septin-like fusion protein MSF-A; MSF; MSF1; NAPB; Ov/Br septin; Ovarian/breast septin alpha; Ovarian/Breast septin; PNUTL4; SEPT9; SEPT9_HUMAN; SeptD1; Septin 9; Septin D1; Septin-9; SINT1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-586
  • 氨基酸序列
    MKKSYSGGTR TSSGRLRRLG DSSGPALKRS FEVEEVETPN STPPRRVQTP LLRATVASST QKFQDLGVKN SEPSARHVDS LSQRSPKASL RRVELSGPKA AEPVSRRTEL SIDISSKQVE NAGAIGPSRF GLKRAEVLGH KTPEPAPRRT EITIVKPQES AHRRMEPPAS KVPEVPTAPA TDAAPKRVEI QMPKPAEAPT APSPAQTLEN SEPAPVSQLQ SRLEPKPQPP VAEATPRSQE ATEAAPSCVG DMADTPRDAG LKQAPASRNE KAPVDFGYVG IDSILEQMRR KAMKQGFEFN IMVVGQSGLG KSTLINTLFK SKISRKSVQP TSEERIPKTI EIKSITHDIE EKGVRMKLTV IDTPGFGDHI NNENCWQPIM KFINDQYEKY LQEEVNINRK KRIPDTRVHC CLYFIPATGH SLRPLDIEFM KRLSKVVNIV PVIAKADTLT LEERVHFKQR ITADLLSNGI DVYPQKEFDE DSEDRLVNEK FREMIPFAVV GSDHEYQVNG KRILGRKTKW GTIEVENTTH CEFAYLRDLL IRTHMQNIKD ITSSIHFEAY RVKRLNEGSS AMANGMEEKE PEAPEM
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Filament-forming cytoskeletal GTPase. May play a role in cytokinesis (Potential). May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri.
  • 基因功能参考文献：
    1. Thus our data indicate that Sept9_i2 is a negative regulator of breast tumorigenesis. We propose that Sept9 tumorigenic properties depend on the balance between Sept9_i1 and Sept9_i2 expression levels. PMID: 28338090
    2. The plasma levels of septin-9 and clusterin in ovarian cancer patients were abnormally elevated, which might be used as potential candidates of peripheral blood tumor biomarkers for early diagnosis of EOC and septin-9 might be related to distal metastases of EOC. PMID: 29970704
    3. The primary aim of this study was to evaluate the diagnostic accuracy of a PCR-based assay for the analysis of SEPT9 promoter methylation in circulating cell-free DNA (mSEPT9) for diagnosing hepatocellular carcinoma. PMID: 29627389
    4. Septin 9 regulates lipid droplet growth through binding to phosphatidylinositol-5-phosphate in Hepatitis C virus infected cells.Septin 9 regulates lipid droplets growth and perinuclear accumulation in a manner dependent on dynamic microtubules.Septin 9 regulates Hepatitis C virus replication. PMID: 27417143
    5. The SEPT9 assay exhibited satisfactory performance in colorectal cancer diagnosis and screening, while more evidence is needed for therapeutic effect monitoring and prognosis prediction. PMID: 28128742
    6. Studies indicate that methylated Septin 9 ((m)SEPT9) can be consistently detected in plasma samples derived from whole blood samples collected with S-Monovette(R) K3E and BD Vacutainer (R) K2EDTA tubes stored at 2-8 degrees C for a maximum of 24 h and for samples collected in S-Monovette CPDA tubes stored at 18-25 degrees C for up to 48 h. PMID: 27753040
    7. our study has validated a new SEPT9 assay and combined testing as an aid in cancer detection, providing a new approach for opportunistic CRC screening. PMID: 27133379
    8. Study shows a stepwise increase of SEPT9 methylation from non-cancerous to cancerous tissue in colorectal adenocarcinoma. PMID: 27660666
    9. Study found SHOX2 and SEPT9 frequently methylated in biliary tract cancers. PMID: 27999621
    10. These results indicate that SEPT9_v2 promoter hypermethylation, which silences the expression of SEPT9_v2 mRNA, is observed in a significant proportion of breast tumors, and that methylated SEPT9_v2 may serve as a novel tumor marker for breast cancer. PMID: 27499429
    11. The first evidence of an interaction between septins and a nonmitotic kinesin is provided and it is suggested that SEPT9 modulates the interactions of KIF17 with membrane cargo. PMID: 26823018
    12. SEPT9 promoter methylation and MN frequency, both measured in peripheral blood, occur at an early stage compared to carcinoma development, indicating that the approach might be suitable to monitor CRC development. PMID: 26633373
    13. KRAS mutations and SEPT9 promoter methylation were present in 34% (29/85) and in 82% (70/85) of primary tumor tissue samples. PMID: 25946211
    14. SEPT9_i1 is expressed in high-grade prostate tumors suggesting it has a significant role in prostate tumorigenesis. PMID: 25898316
    15. we demonstrate that SEPT9 negatively regulates EGFR degradation by preventing the association of the ubiquitin ligase Cbl with CIN85, resulting in reduced EGFR ubiquitylation PMID: 25472714
    16. provide a full overview of the theoretical basis, development, validation, and clinical applications of the SEPT9 assay for both basic science researchers and clinical practitioners PMID: 26471083
    17. Results show that in plasma samples, elevated methylated SEPT9 (mSEPT9) values were detected in colorectal cancer, but not in adenomas. Tissue levels of mSEPT9 alone are not sufficient to predict mSEPT9 levels in plasma. PMID: 25526039
    18. s report here that the septins SEPT2, -9, -11, and probably -7 form fibrillar structures around the chlamydial inclusion. PMID: 25293760
    19. Our results suggest that SEPT9 gene methylation is a valuable biomarker for screening CRC in the Chinese population PMID: 24535900
    20. SEPT9 promoter methylation is detected in free circulating DNA of lung cancer patients PMID: 24633736
    21. The study shows increased SEPT9 expression as a consequence of genomic amplification and is the first study to profile SEPT9_v1 through SEPT9_v7 isoform-specific mRNA expression in tumor and nontumor tissues from patients with breast cancer. PMID: 24127542
    22. prognostic value of SHOX2 and SEPT9 DNA methylation in benign, paramalignant and malignant pleural effusions PMID: 24386354
    23. epigenetic deregulation of SEPT9 plays a role in the development of colorectal cancer. Aberrant hypermethylation of this gene occurs only in one of its CpG islands and this hypermethylation likely is an early event in the adenoma-carcinoma sequence. PMID: 23988185
    24. SEPT9 repeat motifs bind and bundle MTs, and thereby promote asymmetric neurite growth. PMID: 24344182
    25. SEPT9 isoform 1 is required for the association between HIF-1alpha and importin-alpha to promote efficient nuclear translocation. PMID: 24067372
    26. SEPT2 forms a 1:1:1 complex with SEPT7 and SEPT9. PMID: 23572511
    27. Serum methylation levels of TAC1, SEPT9, and EYA4 were significant discriminants between stage I colorectal cancer and healthy controls. PMID: 23862763
    28. SEPT9 plays multiple roles in abscission, one of which is regulated by the action of Cdk1 and Pin1. PMID: 23990466
    29. The identification of a SEPT9 mutation in a neonate with respiratory distress due to vocal cord paralysis expands the differential diagnosis in these patients. PMID: 22981636
    30. Matrix stiffness regulates endothelial cell proliferation through septin 9 PMID: 23118862
    31. SEPT9 in plasma has a role in both left- and right-sided colon cancers PMID: 23049919
    32. Myeloid K562 cells express three SEPT9 isoforms, all of which have an equal propensity to hetero-oligomerize with SEPT7-containing hexamers to generate octameric heteromers. PMID: 22956766
    33. SEPT9_v4 expression may be clinically relevant and contribute to some forms of drug resistance. PMID: 22278362
    34. SEPT9 gene amplification and overexpression during human breast tumorigenesis PMID: 21831286
    35. Data illustrated roles of SEPT9 that might contribute to hetero-trimeric septin complex formation. SEPT9 can substitute for septins of the SEPT2 group and partially for SEPT7. PMID: 21767235
    36. SEPT9 holds a terminal position in the septin octamers, mediating abscission-specific polymerization during cytokinesis. PMID: 22123865
    37. uneven distribution of SEPT9 among core septin heteromers causes heterogeneity with respect to both subunit number and polymerization interfaces PMID: 21737677
    38. Increased methylation of septin 9 resulting in decreased mRNA and protein expression is associated with colorectal cancer. PMID: 21267688
    39. Data demonstrate that SEPT9 mediates the localization of the vesicle-tethering exocyst complex to the midbody, providing mechanistic insight into the role of SEPT9 during cell division. PMID: 21059847
    40. A total of seven heterogeneous SEPT9 duplications have been identified in this study as a causative factor of hereditary neuralgic amyotrophy. PMID: 19939853
    41. New insights and validation are provided for applying SEPT9 transcript variant 1 as a potential target for antitumor therapy via interruption of the HIF-1 pathway. PMID: 20407014
    42. Data show that methylated DNA from advanced precancerous colorectal lesions can be detected using a panel of two DNA methylation markers, ALX4 and SEPT9. PMID: 20140221
    43. Case represents an additional MLL-SEPT9-positive AML that was considered to be related to therapy. RT-PCR and sequencing analyses demonstrated MLL-SEPT9 fusion transcripts with the breakpoint of MLL exon 8/SEPT9 exon 2 and MLL exon 9/SEPT9 exon 2. PMID: 20682395
    44. Results verify IRX1, EBF3, SLC5A8, SEPT9, and FUSSEL18 as valid methylation markers in two separate sets of HNSCC specimens; also preliminarily show a trend between HPV16 positivity and target gene hypermethylation of IRX1, EBF3, SLC5A8, and SEPT9. PMID: 20029986
    45. Coexistence of alternative MLL-SEPT9 fusion transcripts in an acute myeloid leukemia with t(11;17)(q23;q25). PMID: 20113838
    46. Fusion of MLL and MSF in adult de novo acute myelomonocytic leukemia (M4) with t(11;17)(q23;q25). PMID: 12095151
    47. These results reveal that MSF is required for the completion of cytokinesis and suggest a role that is distinct from that of Nedd5. PMID: 12388755
    48. Filament formation of MSF-A, a mammalian septin, in human mammary epithelial cells depends on interactions with microtubules PMID: 12626509
    49. Sept7/9b/11 form a complex that has effects on filament elongation, bundling, or disruption PMID: 15485874
    50. overexpression of SEPT9 in neoplasia is not simply a proliferation-associated phenomenon, despite its role in cytokines PMID: 15782116

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  • 相关疾病：
    Hereditary neuralgic amyotrophy (HNA)
  • 亚细胞定位：
    Cytoplasm, cytoskeleton. Note=In an epithelial cell line, concentrates at cell-cell contact areas. After TGF-beta1 treatment and induction of epithelial to mesenchymal transition, colocalizes partly with actin stress fibers. During bacterial infection, displays a collar shape structure next to actin at the pole of invading bacteria.
  • 蛋白家族：
    TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily, Septin GTPase family
  • 组织特异性：
    Widely expressed. Isoforms are differentially expressed in testes, kidney, liver heart, spleen, brain, peripheral blood leukocytes, skeletal muscle and kidney. Specific isoforms appear to demonstrate tissue specificity. Isoform 5 is the most highly expres
  • 数据库链接：

    HGNC: 7323

    OMIM: 162100

    KEGG: hsa:10801

    STRING: 9606.ENSP00000391249

    UniGene: Hs.440932