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  4. Recombinant Human Serine/threonine-protein kinase Nek8 (NEK8), partial

Recombinant Human Serine/threonine-protein kinase Nek8 (NEK8), partial

  • 货号：
    CSB-YP801219HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP801219HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP801219HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP801219HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP801219HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NEK8
  • Uniprot No.：
  • 别名：
    JCK; NEK 8; NEK12A; NEK8; NEK8_HUMAN; Nephrocystin 9; Never in mitosis A-related kinase 8; NIMA (never in mitosis gene a) related kinase 8; NIMA related kinase 8; NIMA-family kinase NEK8; NIMA-related kinase 12a ; Nima-related protein kinase 12a; NimA-related protein kinase 8; NPHP9; Serine/threonine-protein kinase Nek8
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for renal tubular integrity. May regulate local cytoskeletal structure in kidney tubule epithelial cells. May regulate ciliary biogenesis through targeting of proteins to the cilia. Plays a role in organogenesis and is involved in the regulation of the Hippo signaling pathway.
  • 基因功能参考文献：
    1. NEK8 plays a critical role in replication fork stability through its regulation of the DNA repair and replication fork protection protein RAD51. PMID: 27892797
    2. The mutations: c.2069_2070insC variant (p.Ter693LeufsTer86), and a c.1043C>T variant (p.Thr348Met) in RCC1 domain of NEK8 in two brothers with cardiac, renal, and hepatic anomalies PMID: 26697755
    3. our study demonstrates that NEK8 human mutations cause major organ developmental defects due to altered ciliogenesis and cell differentiation/proliferation through deregulation of the Hippo pathway PMID: 26967905
    4. NEK8 may be a new target gene of HIFs; pVHL can down-regulate NEK8 via HIFs to maintain the primary cilia structure in human renal cancer cells PMID: 25451921
    5. NEK8 is essential for organ development and that the complete loss of NEK8 perturbs multiple signalling pathways resulting in a severe early embryonic phenotype. PMID: 23418306
    6. Mutation in NEK8 is associated with renal ciliopathies PMID: 23973373
    7. ANKS6 as a new NPHP family member that assembles a distinct module of nephronophthisis-associated proteins, encompassing NEK8, INVS and NPHP3. PMID: 23793029
    8. NPHP9 promotes signalling through the transcriptional co-activator TAZ. PMID: 23026745
    9. study finds that induction of ciliogenesis upon cell cycle exit is accompanied by both activation and proteasomal degradation of Nek8, and that activation is dependent upon phosphorylation within the catalytic domain PMID: 22106379
    10. Observational study of gene-disease association. (HuGE Navigator) PMID: 21068128
    11. Data demonstrate for the first time that Nek8 is a novel tumor associated gene, and shares considerable sequence homology with the Nek family of protein kinases and may be involved in G(2)/M progression. PMID: 15019993
    12. mutations cause nephronophthisis; mutant forms show defects in ciliary localization PMID: 18199800
    13. characterization of the proteome in mice that have a double point mutation in the related gene. PMID: 15872312

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  • 相关疾病：
    Nephronophthisis 9 (NPHP9); Renal-hepatic-pancreatic dysplasia 2 (RHPD2)
  • 亚细胞定位：
    Cytoplasm. Cytoplasm, cytoskeleton. Cell projection, cilium. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
  • 蛋白家族：
    Protein kinase superfamily, NEK Ser/Thr protein kinase family, NIMA subfamily
  • 组织特异性：
    Highest expression in thyroid, adrenal gland and skin. Low levels in spleen, colon and uterus. Overexpressed in breast tumors, with highest expression in infiltrating ductal carcinomas and moderate levels in mucinous adenocarcinoma.
  • 数据库链接：

    HGNC: 13387

    OMIM: 609799

    KEGG: hsa:284086

    STRING: 9606.ENSP00000268766

    UniGene: Hs.448468