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  4. Recombinant Human Serine/threonine-protein kinase PAK 2 (PAK2)

Recombinant Human Serine/threonine-protein kinase PAK 2 (PAK2)

  • 货号：
    CSB-YP622641HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP622641HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP622641HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP622641HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP622641HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    C-t-PAK2; CB422; EC 2.7.11.1 ; Gamma PAK; Gamma-PAK; hPAK65; Kinase; p21 (CDKN1A) activated kinase 2; p21 (CDKN1A)-activated kinase 2a; p21 activated kinase 2 ; p21 protein (Cdc42/Rac)-activated kinase 2; p21 protein Cdc42 Rac activated kinase 2; p21-activated kinase 2; p21-activated kinase; 65-KD; p21-activated protein kinase I; p21CDKN1A activated kinase 2; p27; p34; p58; p65PAK; PAK 2 ; PAK-2; PAK-2p34; Pak2; PAK2_HUMAN; PAK65; PAKgamma; S6 H4 kinase; S6/H4 kinase; Serine threonine protein kinase PAK 2; Serine/threonine protein kinase PAK 2
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-524
  • 氨基酸序列
    SDNGELEDK PPAPPVRMSS TIFSTGGKDP LSANHSLKPL PSVPEEKKPR HKIISIFSGT EKGSKKKEKE RPEISPPSDF EHTIHVGFDA VTGEFTGMPE QWARLLQTSN ITKLEQKKNP QAVLDVLKFY DSNTVKQKYL SFTPPEKDGF PSGTPALNAK GTEAPAVVTE EEDDDEETAP PVIAPRPDHT KSIYTRSVID PVPAPVGDSH VDGAAKSLDK QKKKTKMTDE EIMEKLRTIV SIGDPKKKYT RYEKIGQGAS GTVFTATDVA LGQEVAIKQI NLQKQPKKEL IINEILVMKE LKNPNIVNFL DSYLVGDELF VVMEYLAGGS LTDVVTETCM DEAQIAAVCR ECLQALEFLH ANQVIHRDIK SDNVLLGMEG SVKLTDFGFC AQITPEQSKR STMVGTPYWM APEVVTRKAY GPKVDIWSLG IMAIEMVEGE PPYLNENPLR ALYLIATNGT PELQNPEKLS PIFRDFLNRC LEMDVEKRGS AKELLQHPFL KLAKPLSSLT PLIMAAKEAM KSNR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis or proliferation. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Full-length PAK2 stimulates cell survival and cell growth. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Phosphorylates JUN and plays an important role in EGF-induced cell proliferation. Phosphorylates many other substrates including histone H4 to promote assembly of H3.3 and H4 into nucleosomes, BAD, ribosomal protein S6, or MBP. Additionally, associates with ARHGEF7 and GIT1 to perform kinase-independent functions such as spindle orientation control during mitosis. On the other hand, apoptotic stimuli such as DNA damage lead to caspase-mediated cleavage of PAK2, generating PAK-2p34, an active p34 fragment that translocates to the nucleus and promotes cellular apoptosis involving the JNK signaling pathway. Caspase-activated PAK2 phosphorylates MKNK1 and reduces cellular translation.
  • 基因功能参考文献：
    1. Overexpression of PAK2 in oral squamous cell carcinomas may be associated with an advanced pathology grade. PMID: 29714078
    2. the promotion of apoptosis by human cytomegalovirusmiRUS45p in cells was specifically mediated via inhibition of PAK2 expression. PMID: 28765936
    3. leukaemic cells explicitly require PAK2 to grow towards an extracellular matrix. PAK2-deficient cells fail to form colonies in methylcellulose and to induce lymphomas in vivo. PAK2 might therefore be the critical isoform in leukaemic cells by controlling tumour growth PMID: 28707321
    4. Results show that PAK2 kinase plays an alternative anti-apoptotic role, phosphorylating caspase-7 and promoting unfettered cell growth and chemotherapeutic resistance. PMID: 27889207
    5. We found that overexpression of miR-137 inhibited the proliferation of melanoma cells, which could be phenocopied by knockdown of PAK2 using siRNAs. PMID: 26186482
    6. PAK2 is a direct effector of TSC1-TSC2-RHEB signaling and a new target for rational drug therapy in TSC. PMID: 26412398
    7. Nef exploits PAK2 in a stepwise mechanism in which its kinase activity cooperates with an adaptor function for the exocyst complex to inhibit host cell actin dynamics. PMID: 26350970
    8. Cytoplasmic Pak2 may promote cell proliferation in normal endometrium during menstrual cycle. PMID: 26218748
    9. Further analyses show that HDAC6 may promote growth of GBM cells through inhibition of SMAD2 phosphorylation to downregulate p21 PMID: 26150340
    10. Findings indicate that repression of microRNA miR-134 and consequent up-regulation of p21-activated kinase 2 (Pak2) might contribute to paclitaxel resistance. PMID: 26363097
    11. Inhibition of PAK activation at late G2-phase centrosomes caused by Rac1 inactivation coincides with impeded activation of Aurora A and the CyclinB/Cdk1 complex and delayed mitotic entry. PMID: 24840740
    12. Results identified Pak2 as a possibly important mediator of ovarian cancer cell migration on extracellular matrix. PMID: 25050916
    13. PAK2 activation may be associated with advanced tumor progression and poor prognosis of gastric cancer PMID: 24621074
    14. Prostasin repress cancer cells and contribute to chemoresistance by modulating the CASP/PAK2-p34/actin pathway. PMID: 24434518
    15. s demonstrate that HIV-1 Nef expression mediates phosphorylation of Mek1 on serine298 and Pak2 on serine192/197 in T cell lines as well as primary human T cells. PMID: 23746211
    16. thrombin induces monocyte/macrophage migration via PAR1-Galpha12-dependent Pyk2-mediated Gab1 and p115 RhoGEF interactions, leading to Rac1- and RhoA-targeted Pak2 activation. PMID: 24025335
    17. PAK2 negatively modulate TGF-beta signaling by attenuating the receptor-Smad interaction and thus Smad activation PMID: 22393057
    18. Low-to-moderate penetrance protein coding mutations or non-coding mutations at DLG1 and/or PAK2, or a nearby gene, may reproduce the behavioral characteristics of the 3q29 microdeletion. PMID: 21850710
    19. The ability of Nef to associate with PAK2 correlates with the ability to enhance HIV-1 replication. PMID: 21819585
    20. highly expressed PAK2 mediates chemotherapeutic resistance in human breast invasive ductal carcinoma by negatively regulating caspase-7 activity PMID: 21555521
    21. High PAK2 is associated with melanoma. PMID: 21177766
    22. Mechanistic studies of the autoactivation of PAK2: a two-step model of cis initiation followed by trans amplification. PMID: 21098037
    23. analysis of evolutionary conserved residues that are crucial for the catalytic activity of PKA and Pak2 PMID: 20209159
    24. The association between the CD4 receptor and protein kinase pp58 and the protein-tyrosine kinase within the cell introduces a specific pathway by which T lymphocytes become activated. PMID: 20724730
    25. MYO18A is a novel binding partner of the PAK2/betaPIX/GIT1 complex and suggest that MYO18A may play an important role in regulating epithelial cell migration via affecting multiple cell machineries. PMID: 19923322
    26. Knockdown of PAK2 enhances loss of cell-cell junctions and increases lamellipodium extension but does not affect migration speed Hepatocyte Growth Factor (HGF) stimulated DU145 prostate carcinoma cells. PMID: 19628037
    27. The enzymatic phosphorylation reaction of PAK2 can be best interpreted by a rapid-equilibrium random bi-bi reaction model; the catalysis reaction is partially limited by both the phosphoryl group transfer and the product release steps. PMID: 12549935
    28. The opposing effects of Core protein on the transcription pf P21 might be important in the progression of liver disease in HCV-positive patients. PMID: 12823590
    29. Caspase-activated PAK-2 is regulated by subcellular targeting and proteasomal degradation PMID: 12853446
    30. sites of PAK2 autophosphorylation in the regulatory and the catalytic domains and their kinetic effect; multiple regions of PAK2 are involved in the enzyme-substrate recognition PMID: 12907671
    31. Pak2 phosphorylates Myc at three sites (T358, S373, and T400) and affects Myc functions both in vitro and in vivo PMID: 14749374
    32. Nef induces signal transduction via the recruitment of a signaling machinery including Pak2 into lipid rafts, thereby mimicking a physiological cellular mechanism to initiate the TCR cascade. PMID: 15047825
    33. PAK2 kinase activity is increased in response to TCR stimulation; results suggest a novel role for PAK2 as a positive regulator of T cell activation. PMID: 15187108
    34. interacts with Nef proteins from SIV infected chimpanzees PMID: 15194762
    35. Pak2 has a role in the down-regulation of translation initiation in apoptosis by phosphorylation of Mnk1 PMID: 15234964
    36. PS-GAP is a novel regulator of caspase-activated PAK-2 PMID: 15471851
    37. PAK-2 is activated in 1-LN prostate cancer cells by a proteinase inhibitor, alpha 2-macroglobulin PMID: 15908432
    38. binding of Cdc42 localizes Pak2 to the endoplasmic reticulum, where autophosphorylation alters association of the two proteins PMID: 16204230
    39. Pak2 binds to and phosphorylates initiation factor eIF4G, which inhibits association of eIF4E with m7GTP, reducing translation initiation. PMID: 16281055
    40. Nef protein amino acids at positions 85, 89, 187, 188, and 191 (L, H, S, R, and F in the clade B consensus, respectively) are critical for Pak2 association and activation PMID: 16501114
    41. Posttranslational myristoylation of PAK2 might be part of a unique series of mechanisms involved in the regulation of the later events of apoptosis. PMID: 16617111
    42. c-Abl represents a target downstream of phosphatidylinositol 3-kinase-activated PAK2, which differentiates TGF-beta signaling in fibroblasts and epithelial cell lines. PMID: 16867995
    43. This study of tissue-derived HIV-1 Nefs demonstrates that CD4 and MHC-I downregulation are highly conserved Nef functions, while Pak2 association is variable in late stage AIDS patients. PMID: 16979207
    44. define a new class of PAK-interacting proteins, which play an important role in actin cytoskeletal reorganization PMID: 17543336
    45. interaction of Nef with PAK2 does not play a major role in T-cell activation, viral replication, and apoptosis. PMID: 17881449
    46. protein phosphatase 1alpha can act directly on phosphorylated Thr-402 in the activation loop of PAK2 and down-regulate its kinase activity PMID: 18176785
    47. Data show RNAi-mediated or dominant-negative suppression of Pak2, major regulators of cytoskeletal signaling downstream of Cdc42 or Rac1, markedly inhibits EC lumen and tube formation. PMID: 18319301
    48. Huntingtin exerts anti-apoptotic effects by binding to Pak2, which reduces the abilities of caspase-3 and caspase-8 to cleave Pak2 and convert it into a mediator of cell death. PMID: 19240112
    49. PAK-2 activity controls the apoptotic response by regulating levels of activated caspase 3 and thereby its own cleavage to the proapoptotic PAK-2p34 fragment PMID: 19242610

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  • 亚细胞定位：
    [Serine/threonine-protein kinase PAK 2]: Cytoplasm. Note=MYO18A mediates the cellular distribution of the PAK2-ARHGEF7-GIT1 complex to the inner surface of the cell membrane.; [PAK-2p34]: Nucleus. Cytoplasm, perinuclear region. Membrane; Lipid-anchor. Note=Interaction with ARHGAP10 probably changes PAK-2p34 location to cytoplasmic perinuclear region. Myristoylation changes PAK-2p34 location to the membrane.
  • 蛋白家族：
    Protein kinase superfamily, STE Ser/Thr protein kinase family, STE20 subfamily
  • 组织特异性：
    Ubiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen.
  • 数据库链接：

    HGNC: 8591

    OMIM: 605022

    KEGG: hsa:5062

    STRING: 9606.ENSP00000314067

    UniGene: Hs.518530