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  4. Recombinant Human Serine/threonine-protein kinase PLK3 (PLK3)

Recombinant Human Serine/threonine-protein kinase PLK3 (PLK3)

  • 中文名称：
    人PLK3重组蛋白
  • 货号：
    CSB-YP880972HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人PLK3重组蛋白
  • 货号：
    CSB-EP880972HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人PLK3重组蛋白
  • 货号：
    CSB-EP880972HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人PLK3重组蛋白
  • 货号：
    CSB-BP880972HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人PLK3重组蛋白
  • 货号：
    CSB-MP880972HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    CNK; Cytokine Inducible Kinase; Cytokine-inducible serine/threonine-protein kinase; FGF inducible kinase; FGF-inducible kinase; FNK; PLK-3; Plk3; PLK3_HUMAN; Polo Like Kinase 3; Polo-like kinase 3; PRK; Proliferation Related Kinase; Proliferation-related kinase; Serine/threonine-protein kinase PLK3
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-646
  • 氨基酸序列
    MEPAAGFLSP RPFQRAAAAP APPAGPGPPP SALRGPELEM LAGLPTSDPG RLITDPRSGR TYLKGRLLGK GGFARCYEAT DTETGSAYAV KVIPQSRVAK PHQREKILNE IELHRDLQHR HIVRFSHHFE DADNIYIFLE LCSRKSLAHI WKARHTLLEP EVRYYLRQIL SGLKYLHQRG ILHRDLKLGN FFITENMELK VGDFGLAARL EPPEQRKKTI CGTPNYVAPE VLLRQGHGPE ADVWSLGCVM YTLLCGSPPF ETADLKETYR CIKQVHYTLP ASLSLPARQL LAAILRASPR DRPSIDQILR HDFFTKGYTP DRLPISSCVT VPDLTPPNPA RSLFAKVTKS LFGRKKKSKN HAQERDEVSG LVSGLMRTSV GHQDARPEAP AASGPAPVSL VETAPEDSSP RGTLASSGDG FEEGLTVATV VESALCALRN CIAFMPPAEQ NPAPLAQPEP LVWVSKWVDY SNKFGFGYQL SSRRVAVLFN DGTHMALSAN RKTVHYNPTS TKHFSFSVGA VPRALQPQLG ILRYFASYME QHLMKGGDLP SVEEVEVPAP PLLLQWVKTD QALLMLFSDG TVQVNFYGDH TKLILSGWEP LLVTFVARNR SACTYLASHL RQLGCSPDLR QRLRYALRLL RDRSPA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Serine/threonine-protein kinase involved in cell cycle regulation, response to stress and Golgi disassembly. Polo-like kinases act by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates ATF2, BCL2L1, CDC25A, CDC25C, CHEK2, HIF1A, JUN, p53/TP53, p73/TP73, PTEN, TOP2A and VRK1. Involved in cell cycle regulation: required for entry into S phase and cytokinesis. Phosphorylates BCL2L1, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis. Plays a key role in response to stress: rapidly activated upon stress stimulation, such as ionizing radiation, reactive oxygen species (ROS), hyperosmotic stress, UV irradiation and hypoxia. Involved in DNA damage response and G1/S transition checkpoint by phosphorylating CDC25A, p53/TP53 and p73/TP73. Phosphorylates p53/TP53 in response to reactive oxygen species (ROS), thereby promoting p53/TP53-mediated apoptosis. Phosphorylates CHEK2 in response to DNA damage, promoting the G2/M transition checkpoint. Phosphorylates the transcription factor p73/TP73 in response to DNA damage, leading to inhibit p73/TP73-mediated transcriptional activation and pro-apoptotic functions. Phosphorylates HIF1A and JUN is response to hypoxia. Phosphorylates ATF2 following hyperosmotic stress in corneal epithelium. Also involved in Golgi disassembly during the cell cycle: part of a MEK1/MAP2K1-dependent pathway that induces Golgi fragmentation during mitosis by mediating phosphorylation of VRK1. May participate in endomitotic cell cycle, a form of mitosis in which both karyokinesis and cytokinesis are interrupted and is a hallmark of megakaryocyte differentiation, via its interaction with CIB1.
  • 基因功能参考文献：
    1. Those data indicate that the overexpression of PLK3-mediated degradation of abnormal PrP is largely dependent on chaperone-mediated autophagy pathway. PMID: 27344333
    2. study revealed an interesting mutual regulation between Plk3 and SIAH2 and uncovered a regulatory network that functions to fine-tune the cellular hypoxic response. PMID: 28515325
    3. the differential effects of hypoxic stress on Plk3 activity in Human Limbal Stem and HCE cells. Instead of apoptosis, hypoxic stress suppresses Plk3 activity to protect limbal stem cells from death and to allow the process of Human Limbal Stem cell differentiation. PMID: 27281822
    4. Data suggest that HOXA-AS2 could be an oncogene for gastric cancer partly through suppressing P21, PLK3, and DDIT3 expression. PMID: 26384350
    5. PLK3 predominantly is expressed in Cholangiocarcinomas (CCA) cells and that high PLK3 expression correlates with prolonged overall survival. PMID: 25818805
    6. The role of Plk3 in oncogenesis: the aberrant expression of Plk3 was found in different types of tumors. PMID: 25915845
    7. hyperosmotic stress-activated Plk3 elicited gammaH2AX. PMID: 25202016
    8. Plk3 binds to CtIP phosphorylated at S327 via its Polo box domains, which is necessary for robust damage-induced CtIP phosphorylation at S327 and subsequent CtIP phosphorylation at T847. PMID: 25267294
    9. have identified and validated as in vitro PLK2 and PLK3 substrates HSP90, GRP-94, beta-tubulin, calumenin, and 14-3-3 epsilon PMID: 22828320
    10. Here, the s report phosphorylation of Thr4 by Polo-like kinase 3 in mammalian cells. PMID: 22549466
    11. Bcl-xL phosphorylation at Ser49 by polo-like kinase 3 during cell cycle progression and checkpoints PMID: 21840391
    12. Calcium- and integrin-binding protein 1 regulates microtubule organization and centrosome segregation through polo like kinase 3 during cell cycle progression. PMID: 20951827
    13. hyperosmotic stress can activate the Plk3 signaling pathway that subsequently regulates the AP-1 complex by directly phosphorylating ATF-2 independent from the effects of JNK and p38 activation. PMID: 21098032
    14. Plk3 functions as an essential component of the hypoxia regulatory pathway by direct phosphorylation of HIF-1alpha PMID: 20889502
    15. upregulation of CIB1 in cancer cells may inhibit Plk3 activity, leading to abnormal cell cycle regulation in breast cancer cells. PMID: 20473878
    16. Plk3 as a new player in the regulation of the PI3K/PDK1/Akt signaling axis by phosphorylation and stabilization of PTEN. PMID: 20940307
    17. The Plk3 overexpression suppressed cellular growth in a long-term colony-forming assay. And overexpression of Plk3 caused long-term growth suppression in Ras-transformed NIH3T3. PMID: 19556223
    18. These results suggest that Polo-like kinase regulates the dissociation of cohesin from chromosomes early in mitosis. (cohesin) PMID: 11931760
    19. Results suggest that Plk3 plays an important role in the regulation of microtubule dynamics and centrosomal function in the cell and that deregulated expression of Plk3 results in cell cycle arrest and apoptosis. PMID: 11971976
    20. Plk3 is a multifunctional protein that associates with multiple complexes and that contributes to response to stress incurred by DNA damage and mitotic spindle disruption, albeit via different pathways. PMID: 12242661
    21. Plk3 is rapidly activated by reactive oxygen species in fibroblasts, correlating with increased p53 protein levels. Plk3 physically interacts with Chk2 and the interaction is enhanced upon DNA damage. PMID: 12548019
    22. Plk3 has a role in coordinating the translocation and perhaps the timing of both Cdc25C and its target Cdc2/cyclin B to the nucleus upon entry into mitosis PMID: 14968113
    23. Plk3 may be a key protein kinase mediating MEK1 function in the Golgi fragmentation pathway during cell division. PMID: 15021912
    24. Plk3 is a RelA-NF-kappaB-regulated gene that induces apoptosis in both p53-dependent and -independent signaling pathways PMID: 15671037
    25. Introduction of plk3 gene into tumor cells with RV vectors can slow down cell proliferation, prevent cells into cell cycle and protect cells from apoptosis in serum-free culture. PMID: 16388739
    26. the Polo box domain of Plk3 is more potent in inhibiting cell proliferation and inducing apoptosis than that of Plk1 PMID: 16478733
    27. Plk3 is in a pathway linking ATM, Plk3, Chk2, Cdc25C and Cdc2 in cellular response to DNA damage. PMID: 16481012
    28. Plk3 localizes to the nucleolus and is involved in regulation of the G1/S phase transition. PMID: 17264206
    29. Results show that Plk3 mediates UV irradiation-induced c-Jun activation by phosphorylating c-Jun, suggesting that Plk3 plays an important role in mediating programmed cell death of corneal epithelial cells after UV irradiation. PMID: 17804415
    30. topoisomerase IIalpha is a novel physiological substrate for Plk3, and Plk1 and Plk3 play different roles in cell-cycle regulation. PMID: 18062778
    31. hypoxia/reoxygenation induces Plk3 activation instead of the JNK effect to directly phosphorylate and activate c-Jun, subsequently contributing to apoptosis in human corneal epithelial cells PMID: 18650425
    32. The Plk3-VRK1 kinase module might represent two consecutive steps of a signaling cascade that participates in the regulation of Golgi fragmentation. PMID: 19103756

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Nucleus, nucleolus. Golgi apparatus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family, CDC5/Polo subfamily
  • 组织特异性：
    Transcripts are highly detected in placenta, lung, followed by skeletal muscle, heart, pancreas, ovaries and kidney and weakly detected in liver and brain. May have a short half-live. In cells of hematopoietic origin, strongly and exclusively detected in
  • 数据库链接：

    HGNC: 2154

    OMIM: 602913

    KEGG: hsa:1263

    STRING: 9606.ENSP00000361275

    UniGene: Hs.632415