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  4. Recombinant Human Serine/threonine-protein kinase Sgk1 (SGK1)

Recombinant Human Serine/threonine-protein kinase Sgk1 (SGK1)

  • 货号：
    CSB-YP021189HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP021189HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP021189HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP021189HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP021189HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    OTTHUMP00000017247; Serine/threonine protein kinase SGK ; Serine/threonine protein kinase Sgk1; Serine/threonine-protein kinase Sgk1; Serum and glucocorticoid regulated kinase; Serum/glucocorticoid regulated kinase 1; Serum/glucocorticoid regulated kinase; Serum/glucocorticoid-regulated kinase 1; SGK 1; SGK; SGK1; Sgk1 variant i3; SGK1_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-431
  • 氨基酸序列
    MTVKTEAAKG TLTYSRMRGM VAILIAFMKQ RRMGLNDFIQ KIANNSYACK HPEVQSILKI SQPQEPELMN ANPSPPPSPS QQINLGPSSN PHAKPSDFHF LKVIGKGSFG KVLLARHKAE EVFYAVKVLQ KKAILKKKEE KHIMSERNVL LKNVKHPFLV GLHFSFQTAD KLYFVLDYIN GGELFYHLQR ERCFLEPRAR FYAAEIASAL GYLHSLNIVY RDLKPENILL DSQGHIVLTD FGLCKENIEH NSTTSTFCGT PEYLAPEVLH KQPYDRTVDW WCLGAVLYEM LYGLPPFYSR NTAEMYDNIL NKPLQLKPNI TNSARHLLEG LLQKDRTKRL GAKDDFMEIK SHVFFSLINW DDLINKKITP PFNPNVSGPN DLRHFDPEFT EEPVPNSIGK SPDSVLVTAS VKEAAEAFLG FSYAPPTDSF L
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cellular enzymes, transcription factors, neuronal excitability, cell growth, proliferation, survival, migration and apoptosis. Plays an important role in cellular stress response. Contributes to regulation of renal Na(+) retention, renal K(+) elimination, salt appetite, gastric acid secretion, intestinal Na(+)/H(+) exchange and nutrient transport, insulin-dependent salt sensitivity of blood pressure, salt sensitivity of peripheral glucose uptake, cardiac repolarization and memory consolidation. Up-regulates Na(+) channels: SCNN1A/ENAC, SCN5A and ASIC1/ACCN2, K(+) channels: KCNJ1/ROMK1, KCNA1-5, KCNQ1-5 and KCNE1, epithelial Ca(2+) channels: TRPV5 and TRPV6, chloride channels: BSND, CLCN2 and CFTR, glutamate transporters: SLC1A3/EAAT1, SLC1A2 /EAAT2, SLC1A1/EAAT3, SLC1A6/EAAT4 and SLC1A7/EAAT5, amino acid transporters: SLC1A5/ASCT2, SLC38A1/SN1 and SLC6A19, creatine transporter: SLC6A8, Na(+)/dicarboxylate cotransporter: SLC13A2/NADC1, Na(+)-dependent phosphate cotransporter: SLC34A2/NAPI-2B, glutamate receptor: GRIK2/GLUR6. Up-regulates carriers: SLC9A3/NHE3, SLC12A1/NKCC2, SLC12A3/NCC, SLC5A3/SMIT, SLC2A1/GLUT1, SLC5A1/SGLT1 and SLC15A2/PEPT2. Regulates enzymes: GSK3A/B, PMM2 and Na(+)/K(+) ATPase, and transcription factors: CTNNB1 and nuclear factor NF-kappa-B. Stimulates sodium transport into epithelial cells by enhancing the stability and expression of SCNN1A/ENAC. This is achieved by phosphorylating the NEDD4L ubiquitin E3 ligase, promoting its interaction with 14-3-3 proteins, thereby preventing it from binding to SCNN1A/ENAC and targeting it for degradation. Regulates store-operated Ca(+2) entry (SOCE) by stimulating ORAI1 and STIM1. Regulates KCNJ1/ROMK1 directly via its phosphorylation or indirectly via increased interaction with SLC9A3R2/NHERF2. Phosphorylates MDM2 and activates MDM2-dependent ubiquitination of p53/TP53. Phosphorylates MAPT/TAU and mediates microtubule depolymerization and neurite formation in hippocampal neurons. Phosphorylates SLC2A4/GLUT4 and up-regulates its activity. Phosphorylates APBB1/FE65 and promotes its localization to the nucleus. Phosphorylates MAPK1/ERK2 and activates it by enhancing its interaction with MAP2K1/MEK1 and MAP2K2/MEK2. Phosphorylates FBXW7 and plays an inhibitory role in the NOTCH1 signaling. Phosphorylates FOXO1 resulting in its relocalization from the nucleus to the cytoplasm. Phosphorylates FOXO3, promoting its exit from the nucleus and interference with FOXO3-dependent transcription. Phosphorylates BRAF and MAP3K3/MEKK3 and inhibits their activity. Phosphorylates SLC9A3/NHE3 in response to dexamethasone, resulting in its activation and increased localization at the cell membrane. Phosphorylates CREB1. Necessary for vascular remodeling during angiogenesis. Sustained high levels and activity may contribute to conditions such as hypertension and diabetic nephropathy. Isoform 2 exhibited a greater effect on cell plasma membrane expression of SCNN1A/ENAC and Na(+) transport than isoform 1.
  • 基因功能参考文献：
    1. Fewer Tumor Copy Number Segments of the SGK1 Gene Are Associated with Glioblastoma Multiforme. PMID: 29976632
    2. High SGK1 expression is associated with non-small cell lung cancer. PMID: 29328462
    3. Sgk1 stimulated OAT3 transport activity by interfering with the inhibitory effect of Nedd4-2 on the transporter. This study provides important insights into how OAT3-mediated drug elimination is regulated in vivo. PMID: 28608480
    4. Decreased expression of SGK1 may play a critical role in increasing the expression of alpha-syn, which is related with dopaminergic cell death in the Substantia nigra of chronic MPTP-induced Parkinsonism mice and in SH-SY5Y cells. PMID: 29604467
    5. Taken together, our results suggest that SGK1 inhibits PM2.5-induced cell apoptosis and ROS generation via ERK1/2 and AKT signaling pathway in human lung alveolar epithelial A549cells. PMID: 29412164
    6. High SGK1 expression is associated with gastric cancer. PMID: 26942879
    7. associated with risk of hypertension development in Chinese PMID: 27664953
    8. SGK1 was found to be essential for proliferation and survival of thyroid cancer cells harboring PI3K-activating mutations. PMID: 29055016
    9. miRNA-7-5p can regulate the expression of human alveolar ENaC by targeting the mTORC2/SGK-1 signaling pathway. PMID: 27331901
    10. Findings illustrate how cancer cells utilize a chromatin remodeling factor to engage a core survival pathway to support its cancerous phenotypes, and reveal new facets of MTA1-SGK1 axis by a physiologic signal in cancer progression. PMID: 28504714
    11. SGK1 inhibitor SI113 induced a significant reduction in endometrial cancer cells viability, as a result of induction of autophagy, apoptosis, and endoplasmic reticulum stress. PMID: 28177128
    12. Findings show that serum and glucocorticoid-inducible kinase 1 (SGK1) protein dynamics can be an important part of intracellular signaling, directly influencing cellular response decisions. PMID: 28338770
    13. In cancer cells resistant to PI3Kalpha inhibition, PDK1 blockade restores sensitivity to these therapies. SGK1, which is activated by PDK1, contributes to the maintenance of residual mTORC1 activity through direct phosphorylation and inhibition of TSC2. PMID: 27451907
    14. SGK1 promotes YAP/TAZ transcriptional activity. SGK1 enhances YAP/TAZ activity by upregulating YAP/TAZ. SGK1 is a transcriptional target of YAP. SGK1 stabilizes TAZ by inhibiting GSK3beta. PMID: 28634071
    15. Potassium supplementation has a blocking effect against salt-loading-induced IL-17A production in T lymphocytes, and the protective effect was mediated through suppression of p38/MAPK-SGK1 pathway. PMID: 27020669
    16. Akt3 constitutively suppresses macropinocytosis in macrophages through a novel WNK1/SGK1/Cdc42 pathway. PMID: 28389565
    17. Increased expression of SGK1 is associated with Hydrosalpinx. PMID: 26840046
    18. Human SMCT1 is regulated by insulin and SGK1. PMID: 27488665
    19. SGK1 overexpression in tissues and serum was found in patients with endometriosis. PMID: 26827666
    20. SGK1 can mediate chemo- and radio-resistance during the treatment of various human tumors, both in vitro and in vivo. (Review) PMID: 27771704
    21. Up-regulated expression of SGK1 is associated with lung cancer. PMID: 27251632
    22. Study provides evidence that enhanced SGK expression and activity in multiple myeloma cells contributes to resistance to ER stress, including bortezomib challenge. PMID: 26869290
    23. The results from this study may be of particular importance, because SGK1WT over-expression by activating telomerase and reducing reactive oxygen species levels may delay the processes of endothelial senescence. PMID: 26230157
    24. Highly recurrent mutation of GSK1 is associated with nodular lymphocyte predominant Hodgkin lymphoma. PMID: 26658840
    25. data suggest that the induction of SGK1 through treatment with dexamethasone alters MT dynamics to increase Sec5-GEF-H1 interactions, which promote GEF-H1 targeting to adhesion sites. PMID: 26359301
    26. This review focuses on recent advances in understanding of the roles of Akt and SGK1 in the regulation of renal tubular transport. PMID: 26491696
    27. After adjustment for multiple testing, single-nucleotide polymorphism rs9376026 was significantly associated with diastolic blood pressure and mean arterial pressure responses to low-sodium intervention PMID: 26277930
    28. SGK1 is overexpressed in non-small cell lung cancer.SGK1 positively regulates the growth, migration and metastasis of non-small cell lung cancer.SGK1 activates beta-catenin signaling in NSCLC cells. PMID: 26548813
    29. Data suggest that SGK1 expression is down-regulated in prefrontal cortex neurons of post-traumatic stress disorders subjects (male and female; postmortem samples obtained from tissue bank). PMID: 26506154
    30. SGK1 plays a pivotal role in vascular inflammation during atherogenesis. SGK1 participates in the regulation of monocyte/macrophage migration and MMP-9 transcription via regulation of nuclear factor-kappaB. PMID: 25614279
    31. SGK1 was up-regulated in CD4 T cells PMID: 26429539
    32. Increased SGK-1 expression reduces oxidative stress and improves cell survival in endothelial cells. PMID: 24961472
    33. These findings have identified an anti-inflammatory function of SGK1, elucidated the underlying intracellular mechanisms PMID: 25993992
    34. FE65 influences APP degradation via the proteasome, and phosphorylation of FE65 Ser(610) by SGK1 regulates binding of FE65 to APP, APP turnover and processing. PMID: 26188042
    35. we discuss the expression of DISC1, DBZ, and SGK1 , their roles in the regulation of oligodendrocyte function, possible interactions of DISC1 and DBZ in relation to SZ, and the activation of the SGK1 signaling cascade in relation to MDD. PMID: 25705664
    36. mRNA levels of ARC and SGK1 did not differ significantly between the schizophrenia or control samples. PMID: 26038830
    37. MDD patients with low expression of SGK1 have significantly smaller CA2/3 and CA4/DG volumes compared to patients with high expression of SGK1 mRNA and to healthy controls with low/high expression of SGK1, respectively. PMID: 25422956
    38. Inhibition of SGK1 activity as a novel therapeutic approach for the treatment of occlusive vascular diseases. PMID: 25152363
    39. SGK1 overexpression was found to decrease reactive oxygen species generation. PMID: 25825522
    40. C4-CER can replace the PI3K/mTORC2 pathway to directly induce SGK1 to autophosphorylate at Ser422, an initial step leading to activation of PDK1 and of SGK1 by PDK1. PMID: 25384981
    41. PIN1-mediated SGK1 ubiquitination is a major regulator of tamoxifen-resistant breast cancer cell growth and survival PMID: 25667458
    42. Numerical simulations of the model solutions yield a better understanding of the process and indicate the importance of the SGK1 gene in the development of medulloblastoma PMID: 24685888
    43. The role of distinct SGK1/FOXO3A-associated regulation in p53 versus ANXA7 responses were elucidated and proposed that aberrant SGK1 could affect reciprocal SGK1-FOXO3A-Akt regulation. PMID: 24864229
    44. Significant associations between SGK1 and SBP. PMID: 24878720
    45. These results suggested that urinary SGK1 should be a good indicator of tubulointerstitial damage in patients of IgA nephropathy. PMID: 24602173
    46. SGK1 selectively increases wild type-CFTR in the plasma membrane of human airway epithelia cells by inhibiting its endocytic retrieval from the membrane. PMID: 24586903
    47. SGK1 phosphorylated Shank2E, increasing CFTR abundance. PMID: 24811177
    48. Orai1 is expressed in the human endometrium and is up-regulated by SGK1 and TGFbeta1. PMID: 24043696
    49. These data suggest SGK1 plays a key role in regulating neutrophil survival signaling and thus may prove a valuable therapeutic target for the treatment of inflammatory disease. PMID: 24431232
    50. Neuronal expression of SGK1 in aged human brain and its nuclear compartmentalization suggest a possible neuroprotective role. PMID: 23363009

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Endoplasmic reticulum membrane. Cell membrane. Mitochondrion. Note=The subcellular localization is controlled by the cell cycle, as well as by exposure to specific hormones and environmental stress stimuli. In proliferating cells, it shuttles between the nucleus and cytoplasm in synchrony with the cell cycle, and in serum/growth factor-stimulated cells it resides in the nucleus. In contrast, after exposure to environmental stress or treatment with glucocorticoids, it is detected in the cytoplasm and with certain stress conditions is associated with the mitochondria. In osmoregulation through the epithelial sodium channel, it can be localized to the cytoplasmic surface of the cell membrane. Nuclear, upon phosphorylation.; [Isoform 2]: Cell membrane.
  • 蛋白家族：
    Protein kinase superfamily, AGC Ser/Thr protein kinase family
  • 组织特异性：
    Expressed in most tissues with highest levels in the pancreas, followed by placenta, kidney and lung. Isoform 2 is strongly expressed in brain and pancreas, weaker in heart, placenta, lung, liver and skeletal muscle.
  • 数据库链接：

    HGNC: 10810

    OMIM: 602958

    KEGG: hsa:6446

    STRING: 9606.ENSP00000356832

    UniGene: Hs.510078