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  4. Recombinant Human Serine/threonine-protein kinase pim-3 (PIM3)

Recombinant Human Serine/threonine-protein kinase pim-3 (PIM3)

  • 货号：
    CSB-YP771444HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP771444HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP771444HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP771444HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP771444HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PIM3
  • Uniprot No.：
  • 别名：
    Kid 1; Kid1; Kinase induced by depolarization; ONCOGENE PIM3; PIM 3; Pim 3 oncogene; Pim-3 proto-oncogene; serine/threonine kinase; PIM3; Pim3 oncogene; PIM3_HUMAN; Protein kinase Kid 1; Protein kinase Kid1; Serine/threonine kinase Pim 3 ; Serine/threonine kinase Pim3 ; Serine/threonine protein kinase Pim 3; Serine/threonine protein kinase Pim3; Serine/threonine-protein kinase Pim-3
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-326
  • 氨基酸序列
    MLLSKFGSLA HLCGPGGVDH LPVKILQPAK ADKESFEKAY QVGAVLGSGG FGTVYAGSRI ADGLPVAVKH VVKERVTEWG SLGGATVPLE VVLLRKVGAA GGARGVIRLL DWFERPDGFL LVLERPEPAQ DLFDFITERG ALDEPLARRF FAQVLAAVRH CHSCGVVHRD IKDENLLVDL RSGELKLIDF GSGALLKDTV YTDFDGTRVY SPPEWIRYHR YHGRSATVWS LGVLLYDMVC GDIPFEQDEE ILRGRLLFRR RVSPECQQLI RWCLSLRPSE RPSLDQIAAH PWMLGADGGV PESCDLRLCT LDPDDVASTT SSSESL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Proto-oncogene with serine/threonine kinase activity that can prevent apoptosis, promote cell survival and protein translation. May contribute to tumorigenesis through: the delivery of survival signaling through phosphorylation of BAD which induces release of the anti-apoptotic protein Bcl-X(L), the regulation of cell cycle progression, protein synthesis and by regulation of MYC transcriptional activity. Additionally to this role on tumorigenesis, can also negatively regulate insulin secretion by inhibiting the activation of MAPK1/3 (ERK1/2), through SOCS6. Involved also in the control of energy metabolism and regulation of AMPK activity in modulating MYC and PPARGC1A protein levels and cell growth.
  • 基因功能参考文献：
    1. show that PIM1 contributes to melanoma cell proliferation and tumor growth in vivo; however, the presence of PIM2 and PIM3 could also influence the outcome. PMID: 27448973
    2. These results demonstrate the involvement of PIM kinases in LIF-induced regulation in different trophoblastic cell lines which may indicate similar functions in primary cells. PMID: 28729093
    3. PIM kinases in classical Hodgkin lymphoma exhibit pleiotropic effects, orchestrating tumor immune escape and supporting Reed-Sternberg cell survival. PMID: 28698206
    4. Our study suggests that up-regulation of Pim-3 successfully accelerated Chronic Obstructive Pulmonary Disease development, and aggravated lung damage PMID: 28214201
    5. High PIM3 expression is associated with osteosarcoma. PMID: 27826617
    6. The expression status of Pim-3 mRNA was significantly associated with pathological parameters such as pre-surgery prostate specific antigen, Gleason score, pathological stage, and lymphoid metastasis. PMID: 27826135
    7. Pim-3 expression was inversely correlated with that of miR-377. PMID: 27638830
    8. SSRP1/Ets-1/Pim-3 signalling is tightly associated with the proliferation, apoptosis, autophagy, invasion and clonogenicity of nasopharyngeal carcinoma cells, and blockage of this signalling facilitates chemosensitivity of the cells to docetaxel. PMID: 27525970
    9. Pim-3 expression showed a positive correlation with tumor cell differentiation. PMID: 26768612
    10. Pim-3 has a role in human pancreatic cancer cell survival against radiation PMID: 27016481
    11. A high percentage of urothelial carcinomas express Pim kinases. Pim expression differs in NILG, NIHG, and IHG lesions. PMID: 26551340
    12. MicroRNA506 participates in pancreatic cancer pathogenesis by targeting PIM3 PMID: 26238203
    13. Pim-3 kinase enhances growth and metastatic properties of prostate cancer xenografts. PC-3 prostate cancer cells overexpressing either Pim-1 or Pim-3 kinases form larger xenograft tumors than the parental PC-3 cells. PMID: 26075720
    14. Knockdown of Pim-3 by specific shRNA slowed decreased proliferation, induced cell cycle arrest in the G0/G1 phase, and increased apoptosis in glioblastoma cells. PMID: 25817345
    15. PIM3 expression is higher in ovarian cancer than in normal ovarian tissue. Upregulation of PIM3 promotes proliferation and migration of ovarian cancer cells. PMID: 25921139
    16. Data demonstrate the role of PIM kinases in driving myeloid leukemia, and as candidate molecules for therapy against human malignancies. PMID: 25238262
    17. we constructed a dual-function small hairpin RNA (shRNA) vector containing an shRNA targeting Pim-3 and a TLR7-stimulating ssRNA. PMID: 24723452
    18. Blocking the activities of PIM kinases(PIM1, PIM2 and PIM3) could prevent pancreatic cancer development. PIM kinases(PIM1, PIM2 and PIM3) may be a novel target for cancer therapy PMID: 24799066
    19. cancer development and in promoting tumor neovascularization and subsequent tumor growth. Targeting Pim-3 may play a dual role in halting tumor progression, by promoting tumor cell death and blocking angiogenesis PMID: 24789328
    20. Our study indicates that the expression of Pim kinases is physiologically related to DNA-PKcs and ATM in ECs. PMID: 22282239
    21. observations indicate that TCTP enhances Pim-3 stability to simultaneously promote and prevent cell-cycle progression and apoptosis, respectively PMID: 24165482
    22. the existence of different patterns of expression of Pim-3, c-Myc, and p-p27 and their clinicopathologic correlations in adenocarcinomas of the gastric cardia and the distal stomach. PMID: 24488669
    23. Pim-1 and Pim-3 play overlapping but non-identical roles as it relates to gemcitabine sensitivity of pancreatic cancer cells and Pim-3 is upregulated in pancreatic ductal carcinoma. PMID: 23760491
    24. Pim-3 can promote pancreatic tumor growth and angiogenesis by stimulating the VEGF pathway. PMID: 23845873
    25. PIM kinases promote the production of IFNgamma, the hallmark cytokine produced by Th1 cells. PMID: 23209281
    26. Pim protein kinase-3 is regulated by TNF-alpha and promotes endothelial cell sprouting. PMID: 21870113
    27. Pim3 cooperates with other Pim kinases in supporting viability of Myc-induced B-cell lymphomas. PMID: 21646687
    28. Ginsenoside Rg3 decreased both Pim-3 and pBad expressions in a dose-dependent manner in PANC-1 cells. PMID: 19624871
    29. A cell-based test system useful for rapid and inexpensive pre-screening of compounds capable of preventing Pim-mediated phosphorylation is discussed. PMID: 20939820
    30. Pim-3 has an important role in modulating c-Myc and PGC-1alpha protein levels and cell growth PMID: 21187426
    31. Pim-3 alone cannot cause, but can accelerate HCC development. PMID: 20101231
    32. Aberrnntly expressed Pim-3 can cause autonomous cell proliferation or prevent apoptosis in hepatoma cell lines. PMID: 15540201
    33. Pim kinases promote cell cycle progression and tumorigenesis by down-regulating p27(Kip1) expression at both transcriptional and posttranslational levels. PMID: 18593906
    34. Ets-1 can induce aberrant Pim-3 expression and subsequently prevent apoptosis in human pancreatic cancer cells. PMID: 19154409
    35. expression of Pim-1 & Pim-3 is induced in response reactivation in KSHV-infected cells; they are required for KSHV reactivation under these conditions; data indicate Pim-1 & Pim-3 contribute to viral reactivation by phosphorylating the KSHV LANA PMID: 19266083

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Protein kinase superfamily, CAMK Ser/Thr protein kinase family, PIM subfamily
  • 组织特异性：
    Detected in various tissues, including the heart, brain, lung, kidney, spleen, placenta, skeletal muscle, and peripheral blood leukocytes. Not found or barely expressed in the normal adult endoderm-derived organs such as colon, thymus, liver, or small int
  • 数据库链接：

    HGNC: 19310

    OMIM: 610580

    KEGG: hsa:415116

    STRING: 9606.ENSP00000353824

    UniGene: Hs.530381