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  4. Recombinant Human Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform (PPP2R5A)

Recombinant Human Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform (PPP2R5A)

  • 中文名称：
    人PPP2R5A重组蛋白
  • 货号：
    CSB-YP621866HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人PPP2R5A重组蛋白
  • 货号：
    CSB-EP621866HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人PPP2R5A重组蛋白
  • 货号：
    CSB-EP621866HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人PPP2R5A重组蛋白
  • 货号：
    CSB-BP621866HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人PPP2R5A重组蛋白
  • 货号：
    CSB-MP621866HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    2A5A_HUMAN; B56A; MGC131915; PP2A B subunit B' alpha isoform ; PP2A B subunit B56 alpha isoform; PP2A B subunit isoform B''-alpha; PP2A B subunit isoform B56-alpha; PP2A B subunit isoform PR61-alpha; PP2A B subunit isoform R5-alpha; PP2A B subunit PR61 alpha isoform; PP2A B subunit R5 alpha isoform; PPP2R5A; PR61A; PR61alpha; Protein phosphatase 2 regulatory subunit B (B56) alpha isoform; Protein phosphatase 2 regulatory subunit B' alpha; Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-486
  • 氨基酸序列
    SSSSPPAGAASAAISASEKVDGFTRKSVRKAQRQKRSQGSSQFRSQGSQAELHPLPQLKDATSNEQQELFCQKLQQCCILFDFMDSVSDLKSKEIKRATLNELVEYVSTNRGVIVESAYSDIVKMISANIFRTLPPSDNPDFDPEEDEPTLEASWPHIQLVYEFFLRFLESPDFQPSIAKRYIDQKFVQQLLELFDSEDPRERDFLKTVLHRIYGKFLGLRAFIRKQINNIFLRFIYETEHFNGVAELLEILGSIINGFALPLKAEHKQFLMKVLIPMHTAKGLALFHAQLAYCVVQFLEKDTTLTEPVIRGLLKFWPKTCSQKEVMFLGEIEEILDVIEPTQFKKIEEPLFKQISKCVSSSHFQVAERALYFWNNEYILSLIEENIDKILPIMFASLYKISKEHWNPTIVALVYNVLKTLMEMNGKLFDDLTSSYKAERQREKKKELEREELWKKLEELKLKKALEKQNSAYNMHSILSNTSAE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
  • 基因功能参考文献：
    1. Studies indicate protein phosphatase 2, regulatory subunit B', alpha isoform protein (PPP2R5A) plays an important role in many cellular activities. PMID: 29175459
    2. Overexpression of miR-218 led to inhibition of PPP2R5A expression, whereas knockdown of miR-218 increased PPP2R5A levels. Introduction of PPP2R5A abrogated miR218-mediated cell survival and drug resistance. PMID: 28849187
    3. A mitotic phosphorylation site on Cdc20, known to be a substrate of PP2A(B56), modulates APC/C(Cdc20) assembly. PMID: 28404789
    4. Results report that BubR1 and RepoMan bind directly to PP2A-B56 using an LSPIxE short linear motif (SLiM), where phosphorylation of the Ser residue enhances binding. RepoMan and BubR1 bind B56 using both hydrophobic and electrostatic interactions. PMID: 27998540
    5. cyclin E is specifically dephosphorylated at S384 by the PP2A-B56 phosphatase, thereby uncoupling cyclin E degradation from cyclin E-CDK2 activity PMID: 28137908
    6. structure of the PP2A B56-BubR1 complex provides important insights into how the B56 subunit directs the recruitment of PP2A to specific targets. PMID: 27350047
    7. results suggest that PKCalpha can modify PP2A activity by phosphorylation of B56alpha at Ser(41). This interplay between PKCalpha and PP2A represents a new mechanism to regulate important cellular functions like cellular Ca(2+) homeostasis. PMID: 24225947
    8. PP2A regulatory subunit Balpha controls endothelial contractility and vessel lumen integrity via regulation of HDAC7. PMID: 23955003
    9. Following TGF-beta1 treatment, recruitment of PP2A-Balpha by TbetaRI increased in benign cells, but not in malignant cells. Inhibition of PP2A in benign cells resulted in an increase in ERK activation and in TGF-beta1 auto-induction after TGF-beta1 treatment. PMID: 21030067
    10. reversible phosphorylation of Dzip1, which is controlled by the antagonistic action of CK2 and B56-containing PP2As, has an important impact on the stability of Gli transcription factors and Hh signaling. PMID: 21878643
    11. Reveal that PPP2R5A, an essential tumour suppressor, tunes the balance of phosphorylation to promote chromosome-spindle interactions during cell division. PMID: 21874008
    12. The PP2A-B'alpha holoenzyme appears to function as an important endogenous regulator of SK1. PMID: 21075214
    13. B56alpha has a role in nuclear export of the catalytic subunit of PP2A PMID: 20378546
    14. The B'alpha subunit of protein phosphatase 2A (PP 2A) as a CHK2 binding partner shows that their interaction is modulated by DNA damage. PMID: 20160490
    15. PKR regulates B56(alpha)-mediated BCL2 phosphatase activity in acute lymphoblastic leukemia-derived REH cells. PMID: 18957415
    16. The B'alpha1 subunit of the serine/threonine protein phosphatase 2A, which binds to cyclin G1, can stabilize cyclin G1 under unstressed conditions and upon DNA damage, as well as inhibit the ability of cyclin G1 to be ubiquitinated. PMID: 18981217
    17. these data indicate that the B subunits alpha and delta are essential for the interaction of PP2A with CaMKIV. PMID: 19538941

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Chromosome, centromere.
  • 蛋白家族：
    Phosphatase 2A regulatory subunit B56 family
  • 组织特异性：
    Widely expressed with the highest expression in heart and skeletal muscle.
  • 数据库链接：

    HGNC: 9309

    OMIM: 601643

    KEGG: hsa:5525

    STRING: 9606.ENSP00000261461

    UniGene: Hs.744012