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  4. Recombinant Human Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform (PPP2R5C)

Recombinant Human Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform (PPP2R5C)

  • 货号：
    CSB-YP619761HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP619761HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP619761HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP619761HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP619761HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    2A5G_HUMAN; B' alpha regulatory subunit; B56G; KIAA0044 ; MGC23064 ; PP2A B subunit B' gamma isoform; PP2A B subunit B56 gamma isoform; PP2A B subunit isoform B' gamma; PP2A B subunit isoform B''-gamma; PP2A B subunit isoform B56-gamma; PP2A B subunit isoform PR61-gamma; PP2A B subunit isoform R5-gamma; PP2A B subunit PR61 gamma isoform; PP2A B subunit R5 gamma isoform; Ppp2r5c; PR61G ; Protein phosphatase 2 regulatory subunit B (B56) gamma isoform; Protein phosphatase 2 regulatory subunit B' gamma; Protein phosphatase 2; regulatory subunit B'; gamma isoform; Renal carcinoma antigen NY REN 29; Renal carcinoma antigen NY-REN-29; Serine/threonine protein phosphatase 2A 56 kDa regulatory subunit gamma isoform ; Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-524
  • 氨基酸序列
    MLTCNKAGSR MVVDAANSNG PFQPVVLLHI RDVPPADQEK LFIQKLRQCC VLFDFVSDPL SDLKWKEVKR AALSEMVEYI THNRNVITEP IYPEVVHMFA VNMFRTLPPS SNPTGAEFDP EEDEPTLEAA WPHLQLVYEF FLRFLESPDF QPNIAKKYID QKFVLQLLEL FDSEDPRERD FLKTTLHRIY GKFLGLRAYI RKQINNIFYR FIYETEHHNG IAELLEILGS IINGFALPLK EEHKIFLLKV LLPLHKVKSL SVYHPQLAYC VVQFLEKDST LTEPVVMALL KYWPKTHSPK EVMFLNELEE ILDVIEPSEF VKIMEPLFRQ LAKCVSSPHF QVAERALYYW NNEYIMSLIS DNAAKILPIM FPSLYRNSKT HWNKTIHGLI YNALKLFMEM NQKLFDDCTQ QFKAEKLKEK LKMKEREEAW VKIENLAKAN PQYTVYSQAS TMSIPVAMET DGPLFEDVQM LRKTVKDEAH QAQKDPKKDR PLARRKSELP QDPHTKKALE AHCRADELAS QDGR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. The PP2A-PPP2R5C holoenzyme may specifically dephosphorylate and activate TP53 and play a role in DNA damage-induced inhibition of cell proliferation. PP2A-PPP2R5C may also regulate the ERK signaling pathway through ERK dephosphorylation.
  • 基因功能参考文献：
    1. PPP2R5C deletion or EGFR mutation that could be responsible for IER3/pERK overexpression was found in at least 8 cases (67% or more) of lung adenocarcinoma. PMID: 26986830
    2. our data suggest that hepatic PPP2R5C represents an important factor in the functional wiring of energy metabolism and the maintenance of a metabolically healthy state. PMID: 26440364
    3. propose that the mechanism for proliferation inhibition and increased apoptosis of K562 cells following PPP2R5C suppression may be related to the alteration of expression profiles of BRAF, AKT2, AKT3, NFKB2 and STAT3 genes PMID: 25888193
    4. Differential expression of the transcripts PPP2R5C connects ubiquitin-proteasome system with infection-inflammation in preterm births and preterm premature rupture of membranes. PMID: 25884766
    5. KIF4A and PP2A-B56G and -B56E create a spatially restricted negative feedback loop counteracting Aurora B in anaphase. PMID: 25512391
    6. Data indicate that the regulatory PP2A subunit B56gamma mediates suppression of NF-kappaB resulting in increased NF-kappaB target gene expression in T cells. PMID: 24719332
    7. Results show that mutations lost B56gamma tumor-suppressive activity by two distinct mechanisms: one is by disrupting interactions with the PP2A AC core and the other with B56gamma-PP2A substrates (p53 and unknown proteins). PMID: 23723076
    8. PPP2R5C-siRNA treatment altered gene expression profiles in malignant T cells PMID: 23941244
    9. structural insight into the PP2A holoenzyme assembly and emphasizes the importance of HEAT repeat 1 in B56gamma-PP2A tumor-suppressive function PMID: 22315229
    10. Overexpression of PPP2R5C in T-cell malignancy as well as in myeloid leukemia cells might relate to its proliferation and differentiation. PMID: 21548944
    11. ataxia-telangiectasia mutated (ATM) directly phosphorylates and specifically regulates B56gamma3, B56gamma2 and B56delta, after DNA damage. PMID: 21460856
    12. the dynamic nuclear distribution of the B56gamma3 regulatory subunit controls nuclear PP2A activity, which regulates cell cycle controllers, such as p27, to restrain cell cycle progression, and may be responsible for the tumor suppressor function of PP2A PMID: 20448040
    13. a mechanistic basis for the p53-dependent and -independent functions of B56gamma-PP2A PMID: 20473327
    14. B56gamma1 alters the dynamic assembly/disassembly process of nuclear speckles in heart cells. PMID: 15778281
    15. Dihydroxyphenylethanol induces apoptosis by activating serine/threonine protein phosphatase PP2A PMID: 16524888
    16. B56gamma, mediates DNA damage-induced dephosphorylation of p53 at Thr55. PMID: 17245430
    17. PP2A-B56gamma1 phosphatase complexes have a role in cardiac local signaling PMID: 17663574
    18. show an important link between ATM activity and the tumor-suppressive function of B56gamma-protein phosphatase 2A PMID: 17967874

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  • 亚细胞定位：
    Nucleus. Chromosome, centromere.
  • 蛋白家族：
    Phosphatase 2A regulatory subunit B56 family
  • 组织特异性：
    Highest levels in heart, skeletal muscle and brain. Lower levels in pancreas, kidney, lung and placenta. Very low levels in liver.
  • 数据库链接：

    HGNC: 9311

    OMIM: 601645

    KEGG: hsa:5527

    STRING: 9606.ENSP00000412324

    UniGene: Hs.368264