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  4. Recombinant Human Serum response factor (SRF)

Recombinant Human Serum response factor (SRF)

  • 货号：
    CSB-EP022659HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP022659HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP022659HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP022659HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Name:SRF
  • Uniprot No.：
  • 别名：
    c fos serum response element binding factor; c fos serum response element binding transcription factor; ELK3; ERP; MCM 1; MCM1; OTTHUMP00000039820; SAP2; Serum response factor; SRF; SRF serum response factor c fos serum response element binding transcription factor; SRF_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-508
  • 氨基酸序列
    MLPTQAGAAA ALGRGSALGG SLNRTPTGRP GGGGGTRGAN GGRVPGNGAG LGPGRLEREA AAAAATTPAP TAGALYSGSE GDSESGEEEE LGAERRGLKR SLSEMEIGMV VGGPEASAAA TGGYGPVSGA VSGAKPGKKT RGRVKIKMEF IDNKLRRYTT FSKRKTGIMK KAYELSTLTG TQVLLLVASE TGHVYTFATR KLQPMITSET GKALIQTCLN SPDSPPRSDP TTDQRMSATG FEETDLTYQV SESDSSGETK DTLKPAFTVT NLPGTTSTIQ TAPSTSTTMQ VSSGPSFPIT NYLAPVSASV SPSAVSSANG TVLKSTGSGP VSSGGLMQLP TSFTLMPGGA VAQQVPVQAI QVHQAPQQAS PSRDSSTDLT QTSSSGTVTL PATIMTSSVP TTVGGHMMYP SPHAVMYAPT SGLGDGSLTV LNAFSQAPST MQVSHSQVQE PGGVPQVFLT ASSGTVQIPV SAVQLHQMAV IGQQAGSSSN LTELQVVNLD TAHSTKSE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Together with MRTFA transcription coactivator, controls expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration. The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G-actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics. Required for cardiac differentiation and maturation.
  • 基因功能参考文献：
    1. miR-647 functions as a tumor metastasis suppressor in gastric cancer by targeting SRF/MYH9 axis. PMID: 28900514
    2. Findings indicate that miR-101-3p suppresses HOTAIR-induced proliferation and invasion through directly targeting SRF in gastric carcinoma cells. PMID: 28251884
    3. promote GC metastasis by facilitating myofibroblast-cancer cell crosstalk PMID: 27323859
    4. High SRF expression is associated with breast cancer. PMID: 26885614
    5. Results identified SRF as one of the transcription factors responsible for docetaxel-resistant prostate cancer where docetaxel treatment increases its transcriptional activity. Its knockdown re-sensitizes resistant cells to docetaxel. PMID: 28249598
    6. NANOG enabled reactivation of the ROCK and Transforming Growth Factor (TGF)-beta pathways-both of which were impaired in senescent cells-leading to ACTIN polymerization, MRTF-A translocation into the nucleus and serum response factor (SRF)-dependent myogenic gene expression. PMID: 27350449
    7. HOTAIR is regulated by the RhoC-MRTF-A-SRF signaling pathway in breast cancer cells. PMID: 28069441
    8. The findings of the work indicate a role for Galphaq and/or Galpha14 and in CCR2a/CCR2b-stimulated Rho A GTPase-mediated serum response factor activation. PMID: 26823487
    9. Blood pressure-associated polymorphism controls ARHGAP42 expression via serum response factor DNA binding PMID: 28112683
    10. Subset of cellular variants of myofibroma and myopericytoma showing a smooth muscle-like immunophenotype and harboring recurrent SRF-RELA gene fusions, which mimic sarcomas with myogenic differentiation. PMID: 28248815
    11. We identified that SRF was a novel target gene for miR-22 by luciferase reporter assay and knockdown of SRF could cause endothelial dysfunction. PMID: 28161397
    12. miR-181a/b is one of the factors involved in VSMC differentiation toward a synthetic phenotype through targeting at SR PMID: 27911586
    13. miR-483-3p is upregulated in Endothelial progenitor cells (EPCs) from deep vein thrombosis patients, and it targets SRF to decrease EPCs migration and tube formation. PMID: 26801758
    14. ADGRG2 constitutively activates RhoA-SRE pathways. PMID: 26321231
    15. FLNA functions as a positive cellular transducer linking actin polymerization to MKL1-SRF activity, counteracting the known repressive complex of MKL1 and monomeric G-actin. PMID: 26554816
    16. The SRF-IL6 axis is the critical mediator of YAP-induced stemness in mammary epithelial cells and breast cancer. PMID: 26671411
    17. Data show that microRNA miR-320a is a key regulator of rtherogenesis, and down-regulating serum response factor (SRF). PMID: 25728840
    18. These data support a central role of the SRF/MRTF pathway in the pathobiology of lung fibrosis. PMID: 25681733
    19. STAT3 protein regulates vascular smooth muscle cell phenotypic switch by interaction with myocardin and SRF. PMID: 26100622
    20. high levels of Myc engage Miz1 in repressive DNA binding complexes and suppress an SRF-dependent transcriptional program that supports survival of epithelial cells. PMID: 25896507
    21. SRF regulates neutrophil migration, integrin activation, and trafficking. Disruption of the SRF pathway results in myelodysplasia and immune dysfunction. PMID: 25402621
    22. Methylation changes of GFRA1, SRF, and ZNF382 may be a potential biomarker set for prediction of gastric carcinoma metastasis. PMID: 25009298
    23. SRF promotes gastric cancer metastasis and the epithelial to mesenchymal transition through miR-199a-5p-mediated downregulation of E-cadherin. PMID: 25080937
    24. Studied a new therapeutic alternative in the treatment of multiresistant lung adenocarcinoma via siRNA-specific transfection of six crucial molecules involved in lung carcinogenesis: SFR, E2F1, Survivin, HIF1, HIF2 and STAT3. PMID: 24627437
    25. These results suggest that SRF is critical for HCC to acquire a mesenchymal phenotype, which leads to resistance against a sorafenib-mediated apoptotic effect. PMID: 24173109
    26. Dilated cardiomyopathy-associated FHOD3 variant impairs the ability to induce activation of transcription factor serum response factor. PMID: 24088304
    27. results demonstrate that serum response factor (SRF) nuclear expression in castration-resistant prostate cancer bone metastases is associated with survival with patients with shortest survival showing high SRF nuclear expression and patients with longest survival having low SRF nuclear expression PMID: 24249383
    28. Androgen-responsive SRF target genes affect CaP cell behavior. PMID: 23576568
    29. The RhoA signaling axis, a well known upstream stimulator of SRF action that harbors drugable targets, conveyed androgen-responsiveness to SRF. PMID: 23469924
    30. Transfection of the NSCLC cell lines with specific siRNAs against SRF, E2F1 and survivin resulted in a very considerable reduction of the intracellular mRNA concentration. PMID: 23152437
    31. Itk enhances Galpha13 mediated activation of serum response factor (SRF) transcriptional activity dependent on its ability to interact with Galpha13, but its kinase activity is not required to enhance SRF activity. PMID: 23454662
    32. Substitution of any of the TFBS from our particular search of MEF2, CREB and SRF significantly decreased the number of identified clusters PMID: 23382855
    33. High expression of serum response factor is associated with gastric carcinoma. PMID: 23134219
    34. Upon neuronal injury via facial nerve transection, constitutively active SRF enhances motorneuron survival. PMID: 22537405
    35. Dysfunction or loss of the SRF-activating mitogen-associated kinase pathway under stress conditions in transgenic mice may be part of Parkinson's disease etiology. PMID: 22356487
    36. Lmod1 is a new SMC-restricted SRF/MYOCD target gene. PMID: 22157009
    37. Downregulation of the activity of the MRTF-SRF axis and the expression of muscle-specific microRNAs, particularly miR-1, may contribute to COPD-associated skeletal muscle dysfunction. PMID: 21998125
    38. overexpression of serum response factor in hepatocellular carcinoma may play an important role in tumor cell migration and invasion through upregulation of matrix metalloproteinase-2 and matrix metalloproteinase-9 PMID: 21842128
    39. RTVP-1 plays a role in the effect of serum response factor on glioma cell migration PMID: 21777672
    40. Data show that serum response factor (SRF) is one of miR-483-5p target genes. PMID: 21893058
    41. SRF is able to gain nuclear entry through an auxiliary, nuclear localization sequence-independent mechanism PMID: 21131446
    42. These data define serum response factor as a host cell transcription factor that regulates immediate early gene expression in Toxoplasma-infected cells. PMID: 21479245
    43. Serum response factor up-regulation, combined with the presence of co-morbidities, increase the risk of recurrent gastric ulcer bleeding. PMID: 21410985
    44. SRF pathway alterations are linked to insulin resistance, may contribute to type 2 diabetes pathogenesis, and could represent therapeutic targets PMID: 21393865
    45. Data show that the ancestor sequence of SRF- and MEF2-type MADS domains is more similar to MEF2-type MADS domains than to SRF-type MADS domains. PMID: 20724380
    46. Overexpression of SRF in hepatocellular carcinoma (HCC) cells modulates the Wnt/beta-catenin pathway, and this plays an important role in HCC progression. PMID: 20811705
    47. Data reveal that serum response factor is a novel interferon (IFN)gamma-regulated gene and further elucidate the molecular pathway between IFNgamma, IFNgamma-regulated genes, and SRF and its target genes. PMID: 20685657
    48. two transcription factors, SRF and TFAP2, as well as an intronic element encompassing EGR3-like sequence, that work together to regulate expression of the FXN gene PMID: 20808827
    49. SRF depletion affects the expansion of the high and low differentiation grade HCC cells HepG2 and JHH6 PMID: 20144681
    50. Data show that serum response factor is an essential regulator of primary human vascular smooth muscle cell proliferation and senescence. PMID: 20096952

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  • 亚细胞定位：
    Nucleus.
  • 数据库链接：

    HGNC: 11291

    OMIM: 600589

    KEGG: hsa:6722

    STRING: 9606.ENSP00000265354

    UniGene: Hs.520140