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  4. Recombinant Human Sestrin-2 (SESN2)

Recombinant Human Sestrin-2 (SESN2)

  • 货号：
    CSB-YP021096HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP021096HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP021096HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP021096HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP021096HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    DKFZp761M0212; DKFZp761M02121; Hi95; Hypoxia induced gene 95; MGC11758; OTTHUMP00000003641; OTTMUSP00000010184; RP23 63D8.3; SES2; SESN 2; SESN2; SESN2_HUMAN; SEST2; Sestrin 2; Sestrin-2
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-480
  • 氨基酸序列
    MIVADSECRA ELKDYLRFAP GGVGDSGPGE EQRESRARRG PRGPSAFIPV EEVLREGAES LEQHLGLEAL MSSGRVDNLA VVMGLHPDYF TSFWRLHYLL LHTDGPLASS WRHYIAIMAA ARHQCSYLVG SHMAEFLQTG GDPEWLLGLH RAPEKLRKLS EINKLLAHRP WLITKEHIQA LLKTGEHTWS LAELIQALVL LTHCHSLSSF VFGCGILPEG DADGSPAPQA PTPPSEQSSP PSRDPLNNSG GFESARDVEA LMERMQQLQE SLLRDEGTSQ EEMESRFELE KSESLLVTPS ADILEPSPHP DMLCFVEDPT FGYEDFTRRG AQAPPTFRAQ DYTWEDHGYS LIQRLYPEGG QLLDEKFQAA YSLTYNTIAM HSGVDTSVLR RAIWNYIHCV FGIRYDDYDY GEVNQLLERN LKVYIKTVAC YPEKTTRRMY NLFWRHFRHS EKVHVNLLLL EARMQAALLY ALRAITRYMT
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Functions as an intracellular leucine sensor that negatively regulates the TORC1 signaling pathway through the GATOR complex. In absence of leucine, binds the GATOR subcomplex GATOR2 and prevents TORC1 signaling. Binding of leucine to SESN2 disrupts its interaction with GATOR2 thereby activating the TORC1 signaling pathway. This stress-inducible metabolic regulator also plays a role in protection against oxidative and genotoxic stresses. May negatively regulate protein translation in response to endoplasmic reticulum stress, via TORC1. May positively regulate the transcription by NFE2L2 of genes involved in the response to oxidative stress by facilitating the SQSTM1-mediated autophagic degradation of KEAP1. May also mediate TP53 inhibition of TORC1 signaling upon genotoxic stress. Moreover, may prevent the accumulation of reactive oxygen species (ROS) through the alkylhydroperoxide reductase activity born by the N-terminal domain of the protein. Was originally reported to contribute to oxidative stress resistance by reducing PRDX1. However, this could not be confirmed.
  • 基因功能参考文献：
    1. High serum Sestrin 2 levels are associated with metabolic synfrome. PMID: 30099048
    2. Knockdown of sesn2 aggravates atherosclerotic processes by increasing pro-inflammatory reactions and ER stress in the endothelium via an AMPK-dependent mechanism, suggesting that sesn2 might be a novel therapeutic target for atherosclerosis. PMID: 28215577
    3. SESN2 is critical to protect cells against detrimental effect of mitochondrial damage and RBX1 is a negative regulator of SESN2 which hampers its stabilization. PMID: 29294217
    4. Sesn2 ablation increased UVA-induced Nrf2 induction and inhibits UVA-induced ROS production, indicating that Sesn2 acts as an upstream regulator of Nrf2 PMID: 27463837
    5. tribbles pseudokinase 3(TRB3) and sestrin 2 may contribute to the development of obesity and its complications and can be considered interesting therapeutic target for the treatment of obesity PMID: 28639842
    6. Overexpression of Sesn2 downregulated the expression of autophagy genes, upregulated pro-apoptotic gene expression, and downregulated specific extracellular matrix markers in stress-induced cells. PMID: 29518770
    7. These results suggest that sestrin 2 suppresses CRC cell growth through activation of the AMPK/mTORC1 pathway and induction of apoptosis, and could be a novel pharmacological target for the treatment of CRC. PMID: 28525387
    8. This study shows that differential Sestrin2-mediated regulation of mTORC1 and mTORC2 is necessary for the survival of glutamine-depleted lung cancer cells. PMID: 28723563
    9. There was significant overexpression of serum sesn2 protein and mRNA levels in the Alzheimer's Disease group compared to Mild Cognitive Impairment and the control group. PMID: 27567861
    10. isorhapontigenin treatment induces autophagy and inhibits bladder cancer growth through MAPK8-JUN-dependent transcriptional induction of SESN2 PMID: 27171279
    11. simple analysis of the reported "apo"-Sestrin2 structure reveals the clear presence of prominent, unmodeled electron density in the leucine-binding pocket that exactly accommodates the leucine observed in the higher resolution structure. PMID: 27649739
    12. Mass spectrometry analysis, western blot and surface plasmon resonance (SPR) of affinity purified sesn1 and sesn2 proteins confirmed their identity; biophysical characteristics were observed using circular dichroism (CD) showing that sesn1 and sesn2 have a predominant a-helical structure. PMID: 28707664
    13. the results support a model in which leucine selectively promotes dephosphorylation of Sestrin2. PMID: 27010498
    14. The data are the first to indicate that SESN2 might be a novel prognostic marker for hepatocellular carcinoma PMID: 28118855
    15. These results highlight Sestrin2 as a novel tumor suppressor, whose downregulation can accelerate both colitis and colon carcinogenesis. PMID: 26913956
    16. The up-regulation of SESN2 by mitochondrial dysfunction requires binding to ATF4. PMID: 26771712
    17. Sestrin2 silencing strongly inhibits cytokine-induced cell death. PMID: 26313705
    18. Sesn2 has two subdomains. The N-terminal domain reduces alkylhydroperoxide radicals through its helix-turn-helix oxidoreductase motif, and the C-terminal domain interacts with GATOR2 and subsequently inhibits mTORC1. PMID: 26612684
    19. Sestrin2 inhibits mTORC1 through modulation of GATOR complexes PMID: 25819761
    20. Sestrin2: A Promising Therapeutic Target for Liver Diseases PMID: 26133704
    21. SESN2-AMPK signaling could exert a protective effect against glucose deprivation-induced cell death and that this effect is mediated by restoration of mitochondrial function. PMID: 25778901
    22. Sesn2 is a potential tumor suppressor in lung epithelial cells. The expression level of Sesn2 may serve as a prognostic marker for Chinese lung cancer patients in the clinic. PMID: 25962159
    23. These results indicate that Sestrin2 is a leucine sensor for the mTORC1 pathway. PMID: 26449471
    24. this study presents the 2.7 angstrom crystal structure of Sestrin2 in complex with leucine. PMID: 26586190
    25. knockdown of Sestrin2 using small RNA interference promotes cell apoptosis and reactive oxygen species production induced by oxLDL. PMID: 25692450
    26. The findings suggested that a decreased expression of sestrin 2 is associated with an unfavorable prognosis, which suggests that it is a novel and crucial predictor for colorectal cancer metastasis. PMID: 25572852
    27. Sestrin2 has a hepatoprotective role against chronic ER stress PMID: 24947615
    28. an AMPK-independent mechanism of mTORC1 inhibition by Sestrins mediated by their interaction with GATOR2, is reported. PMID: 25457612
    29. GAA promotes FoxO3 nuclear translocation and binding to the SESN2 enhancer. PMID: 25802279
    30. Data indicate that inactivation of Sestrin 2 (Sesn2) or nuclear factor erythroid 2-related factor 2 (Nrf2) induced reactive oxygen species-mediated proteasomal inhibition and platelet-derived growth factor receptor beta (Pdgfrbeta) accumulation. PMID: 25716320
    31. Sesn2 is oncogenic in skin squamous cell carcinoma and melanoma. PMID: 25378405
    32. knockdown of SESN2 using small RNA interference promotes cellular toxicity of angiotensin II, as well as a reduction in cell viability, exacerbation of oxidative stress, and stimulation of apoptosis. PMID: 24838122
    33. Data indicate that sestrin2 expression is upregulated by dopamine D2 receptor. PMID: 25024286
    34. Sestrin2 inhibits uncoupling protein 1 expression through suppressing reactive oxygen species. PMID: 24825887
    35. results show that ATF4-regulated SESN2 expression presents a new link between ER stress and mTOR inhibition and autophagy. PMID: 23916134
    36. results imply that SESN2 could serve as both a biomarker and as a drug target in the clinical management of COPD PMID: 24046361
    37. Sesn2 as a new energetic stress sensor, which appears to be protective against energetic stress-induced apoptosis that integrates the pro-survival function of Akt and the negative regulation of mTOR. PMID: 23238567
    38. The JNK pathway mediated sestrin 2 expression and contributed to autophagy induction, highlighting the relationship of sestrin 2 and autophagy and the JNK signaling pathway. PMID: 22982090
    39. Sestrin-2 redistributes to neuronal soma in human immunodeficiency virus (HIV)-associated neurocognitive disorders. PMID: 22450766
    40. Sesn2 is required for the Nrf2-mediated cytoprotective activity against hydrogen peroxide PMID: 22749810
    41. In breast cancer cells SESN2 is associated with AMPK. PMID: 22363791
    42. Signalling by the AMP-activated protein kinase was also involved as a downstream target of sestrin2. PMID: 21418191
    43. PDGFRbeta accumulation is associated with increased oxidative stress and cellular damage in SESN2 silenced human glioblastoma U87 cells. PMID: 21536039
    44. results suggest that, unlike Srx, Sesn 2 is not a sulfinic Prx reductase PMID: 19113821
    45. results show that sestrins, a family of proteins whose expression is modulated by p53, are required for regeneration of peroxiredoxins containing Cys-SO(2)H PMID: 15105503
    46. depletion of Sestrin2 or Dram failed to affect autophagy in p53-deficient cells PMID: 19377293

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Sestrin family
  • 组织特异性：
    Widely expressed.
  • 数据库链接：

    HGNC: 20746

    OMIM: 607767

    KEGG: hsa:83667

    STRING: 9606.ENSP00000253063

    UniGene: Hs.469543