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  4. Recombinant Human Small kinetochore-associated protein (SKAP)

Recombinant Human Small kinetochore-associated protein (SKAP)

  • 中文名称：
    人KNSTRN重组蛋白
  • 货号：
    CSB-YP896891HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人KNSTRN重组蛋白
  • 货号：
    CSB-EP896891HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人KNSTRN重组蛋白
  • 货号：
    CSB-BP896891HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人KNSTRN重组蛋白
  • 货号：
    CSB-MP896891HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    KNSTRN
  • Uniprot No.：
  • 别名：
    KNSTRN; C15orf23; SKAP; TRAF4AF1; HSD11; Small kinetochore-associated protein; SKAP; Kinetochore-localized astrin-binding protein; Kinastrin; Kinetochore-localized astrin/SPAG5-binding protein; TRAF4-associated factor 1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-316
  • 氨基酸序列
    MAAPEAPPLD RVFRTTWLST ECDSHPLPPS YRKFLFETQA ADLAGGTTVA AGNLLNESEK DCGQDRRAPG VQPCRLVTMT SVVKTVYSLQ PPSALSGGQP ADTQTRATSK SLLPVRSKEV DVSKQLHSGG PENDVTKITK LRRENGQMKA TDTATRRNVR KGYKPLSKQK SEEELKDKNQ LLEAVNKQLH QKLTETQGEL KDLTQKVELL EKFRDNCLAI LESKGLDPAL GSETLASRQE STTDHMDSML LLETLQEELK LFNETAKKQM EELQALKVKL EMKEERVRFL EQQTLCNNQV NDLTTALKEM EQLLEM
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Essential component of the mitotic spindle required for faithful chromosome segregation and progression into anaphase. Promotes the metaphase-to-anaphase transition and is required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture. The astrin (SPAG5)-kinastrin (SKAP) complex promotes stable microtubule-kinetochore attachments. Required for kinetochore oscillations and dynamics of microtubule plus-ends during live cell mitosis, possibly by forming a link between spindle microtubule plus-ends and mitotic chromosomes to achieve faithful cell division. May be involved in UV-induced apoptosis via its interaction with PRPF19; however, these results need additional evidences.
  • 基因功能参考文献：
    1. The s show that the Astrin-SKAP complex binds synergistically to microtubules with the Ndc80 complex to form an integrated interface. PMID: 28841134
    2. Data show that aurora-B regulates end-on conversion in human cells and indicate a late role for SPAG5 protein (Astrin)-SKAP complex in the end-on conversion process. PMID: 28751710
    3. KNSTRN Mutation, a Cutaneous Squamous Carcinoma-Specific Mutation is associated with Solid Tumors and Leukemias. PMID: 26433880
    4. Molecular requirements for the inter-subunit interaction and kinetochore recruitment of SKAP and Astrin have been reported. PMID: 27095104
    5. study reports presence of KNSTRN mutations in basal cell carcinomas (BCC); mutant KNSTRN disrupts sister chromatid cohesion and promotes genomic instability in functional assays; KNSTRN mutations in BCC appear to occur late in disease progression and are preferentially found in advanced tumors PMID: 26348826
    6. Mechanistically, the N-terminal of SKAP binds to EB1, and its C terminus binds to IQGAP1 in migrating cells. PMID: 26242911
    7. These findings suggest that SKAP promotes ultraviolet rays-induced cell apoptosis by negatively regulating the anti-apoptotic protein Prp19. PMID: 24718257
    8. Findings suggest a role for kinetochore protein KNSTRN mutagenesis in cutaneous squamous cell carcinomas (SCCs) development. PMID: 25194279
    9. SKAP constitutes a dynamic link between spindle microtubule plus-ends and mitotic chromosomes to achieve faithful cell division. PMID: 23035123
    10. SKAP cooperates with CENP-E to orchestrate dynamic kinetochore-microtubule interaction for faithful chromosome segregation. PMID: 22110139
    11. This paper identifies role of gene product of FLJ14502, named SKAP in the paper, in mitosis. SKAP promotes metaphase-to-anaphase transition by mediating seperase activation after spindle assembly checkpoint is satisfied in mitosis. PMID: 19667759

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  • 相关疾病：
    Cutaneous squamous cell carcinomas (SCC): A malignancy of the skin. The hallmark of cutaneous SCC is malignant transformation of normal epidermal keratinocytes. Disease susceptibility is associated with variations affecting the gene represented in this entry. Variant Phe-24 appears specific for UV-associated malignancies (PubMed:25194279).
  • 亚细胞定位：
    Nucleus. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle pole.
  • 组织特异性：
    Widely expressed, including in skin.
  • 数据库链接：

    HGNC: 30767

    OMIM: 614718

    KEGG: hsa:90417

    STRING: 9606.ENSP00000249776

    UniGene: Hs.525796