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  4. Recombinant Human Sodium-coupled monocarboxylate transporter 1 (SLC5A8), partial

Recombinant Human Sodium-coupled monocarboxylate transporter 1 (SLC5A8), partial

  • 货号：
    CSB-YP836676HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP836676HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP836676HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP836676HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP836676HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SLC5A8
  • Uniprot No.：
  • 别名：
    AIT; Apical iodide transporter; Electrogenic sodium monocarboxylate cotransporter; MGC125354; SC5A8_HUMAN; SLC5A8; SMCT; SMCT1; sodium coupled monocarboxylate transporter 1; Sodium iodide related cotransporter; Sodium iodide-related cotransporter; Sodium-coupled monocarboxylate transporter 1; solute carrier family 5 iodide transporter member 8; Solute carrier family 5 member 8
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts as an electrogenic sodium (Na(+)) and chloride (Cl-)-dependent sodium-coupled solute transporter, including transport of monocarboxylates (short-chain fatty acids including L-lactate, D-lactate, pyruvate, acetate, propionate, valerate and butyrate), lactate, mocarboxylate drugs (nicotinate, benzoate, salicylate and 5-aminosalicylate) and ketone bodies (beta-D-hydroxybutyrate, acetoacetate and alpha-ketoisocaproate), with a Na(+):substrate stoichiometry of between 4:1 and 2:1. Catalyzes passive carrier mediated diffusion of iodide. Mediates iodide transport from the thyrocyte into the colloid lumen through the apical membrane. May be responsible for the absorption of D-lactate and monocarboxylate drugs from the intestinal tract. Acts as a tumor suppressor, suppressing colony formation in colon cancer, prostate cancer and glioma cell lines. May play a critical role in the entry of L-lactate and ketone bodies into neurons by a process driven by an electrochemical Na(+) gradient and hence contribute to the maintenance of the energy status and function of neurons.
  • 基因功能参考文献：
    1. The present work reveals much lower iodide affinity in the pocket of human SMCT1 analogous to the putative iodide-binding pocket of human NIS. PMID: 29131623
    2. Human SMCT1 is regulated by insulin and SGK1. PMID: 27488665
    3. Data suggest positive correlation between BMI (body mass index)/waist circumference in morbidly obese or obese subjects and jejunal mucosa mRNA abundance of SMCT1; BMI was not significantly correlated with mRNA abundance for glucose transporters SGLT1 and GLUT2 or the low-affinity short-chain fatty acid/monocarboxylate transporter SMCT2. PMID: 27801863
    4. Underexpression of SLC5A8 is associated with hepatocellular carcinoma. PMID: 27465549
    5. Data suggest that SLC5A8, SLC5A5 (sodium-iodide symporter), and SLC26A4 (pendrin), the 3 known iodide transporters, are important in breast tissue metabolism in lactation and in breast neoplasms. [REVIEW] PMID: 26285906
    6. Gene silencing of SLC5A8 by hypermethylation was associated with poor prognosis in cases of node-negative stage I and II lung adenocarcinoma. PMID: 25827678
    7. Data indicate oncogenic HRAS (HRAS(G12V)) as a potent mediator of SLC5A8 silencing in human nontransformed normal mammary epithelial cell lines and in mouse mammary tumors through DNMT1. PMID: 23918800
    8. These results suggest that DNA methylation in the SLC5A8 promoter region may suppress the expression of SLC5A8 in lung tumor. PMID: 23273563
    9. SLC5A8 suppresses tumour progression through depletion of survivin without involving its transport function PMID: 23167260
    10. SLC5A8 nuclear translocation and loss of expression are associated with poor outcome in pancreatic ductal adenocarcinoma. PMID: 22450368
    11. SLC5A8 has a role in prostate cancer risk and aggressiveness PMID: 21802122
    12. SLC5A8 is highly methylated in African American colon adenomas PMID: 21687703
    13. Efficacy of LP or their secreted soluble factors to stimulate SMCT1 expression and function and to counteract the inhibitory effects of TNF-alpha on butyrate absorption could have potential therapeutic value. PMID: 20671196
    14. Sodium-coupled electrogenic transport of pyroglutamate (5-oxoproline) via SLC5A8, a monocarboxylate transporter. PMID: 20211600
    15. results elucidate the mechanism underlying the regulation of SLC5A8 gene transcription and also define a novel regulatory sequence that may be used to increase expression of the SLC5A8 gene in cancer gene therapy PMID: 20082847
    16. SMCT1 possesses a anionic leak current that becomes significant whenever external Na(+) concentration is reduced PMID: 19864324
    17. Identification and characterization of a putative human iodide transporter located at the apical membrane of thyrocytes. (hAIT, human apical iodide transporter) PMID: 12107270
    18. SLC5A8 exon 1 methylation is an early event, detectable in colon adenomas & microscopic aberrant crypt foci. SLC5A8 is a member of the family of sodium solute symporters, now added to the candidate colon cancer suppressor genes. PMID: 12829793
    19. functional identity of SLC5A8 as a Na(+)-coupled transporter for short-chain fatty acids. PMID: 14966140
    20. In thyroid carcinomas, the mean and median hAIT mRNA levels were significantly decreased PMID: 15001644
    21. Data suggest that SLC5A8 functions as a growth suppressor gene in vitro and that it is silenced frequently by epigenetic mechanisms in primary gliomas. PMID: 15867356
    22. the ability of SLC5A8 to deliver butyrate into colonic epithelial cells most likely underlies the tumor suppressive role of this transporter [review] PMID: 16375929
    23. Na(+)/monocarboxylate transport expression may be a favorable indicator of colorectal cancer prognosis PMID: 16670197
    24. Aberrant methylation and hence silencing of TIMP3, SLC5A8, DAPK and RARbeta2, in association with BRAF mutation, may be an important step in PTC tumorigenesis and progression. PMID: 16858683
    25. SMCT1(SLC5A8) may participate in the intestinal absorption of monocarboxylate drugs. PMID: 17245649
    26. biophysical analysis of the stoichiometry for the Na+/monocarboxylate cotransporter SMCT1 PMID: 17526579
    27. DNA methylation in the SLC5A8 promoter region suppressed the expression of SLC5A8 in prostate tumor. PMID: 18037591
    28. SLC5A8 may function as a tumor suppressor gene whose silencing may contribute to carcinogenesis and progression of pancreatic cancer. PMID: 18437076
    29. Frequent methylation of SLC5A8 gene is associated with head and neck squamous cell carcinoma PMID: 18559491
    30. In acute myeloid leukemia with mixed lineage leukemia partial tandem duplication, the SLC5A8 tumor suppressor gene promoter was more frequently hypermethylated. PMID: 18566324
    31. SLC5A8 mediates the tumor-suppressive effects of the bacterial fermentation product butyrate in the colon PMID: 18661192

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  • 亚细胞定位：
    Apical cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Sodium:solute symporter (SSF) (TC 2.A.21) family
  • 组织特异性：
    Expressed in normal thyroid, localized at the apical pole of thyroid cells facing the colloid lumen, but expression profoundly decreased in thyroid carcinomas. Expressed in normal colon but absent in colon aberrant crypt foci and colon cancers. Present in
  • 数据库链接：

    HGNC: 19119

    OMIM: 608044

    KEGG: hsa:160728

    STRING: 9606.ENSP00000445340

    UniGene: Hs.444536