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  4. Recombinant Human Sodium/nucleoside cotransporter 1 (SLC28A1), partial

Recombinant Human Sodium/nucleoside cotransporter 1 (SLC28A1), partial

  • 货号：
    CSB-YP021539HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP021539HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP021539HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP021539HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP021539HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SLC28A1
  • Uniprot No.：
  • 别名：
    CNT 1; CNT1; Concentrative nucleoside transporter 1; hCNT1; Na(+)/nucleoside cotransporter 1; S28A1_HUMAN; SLC28A1; Sodium-coupled nucleoside transporter 1; Sodium/nucleoside cotransporter 1; solute carrier family 28 concentrative nucleoside transporter member 1; Solute carrier family 28 member 1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Sodium-dependent and pyrimidine-selective transporter. Exhibits the transport characteristics of the nucleoside transport system cit or N2 subtype (N2/cit) (selective for pyrimidine nucleosides and adenosine). Transports uridine, cytidine, thymidine, and nucleoside-derived drugs. Transports the antiviral pyrimidine nucleoside analogs 3'-azido-3'-deoxythymidine (AZT) and 2',3'-dideoxycytidine (ddC). It may be involved in the intestinal absorption and renal handling of pyrimidine nucleoside analogs used to treat acquired immunodeficiency syndrome (AIDS). It has the following selective inhibition: adenosine, thymidine, cytidine, uridine >> guanosine, inosine.
  • 基因功能参考文献：
    1. Brazilian Amerindian ancestry compared to Asian, European, and African Genomes.SNPs within or proximal to CIITA (rs6498115), SMC6 (rs1834619), and KLHL29 (rs2288697) were most differentiated in the Amerindian-specific branch. SNPs in ADAMTS9 (rs7631391), DOCK2 (rs77594147), SLC28A1 (rs28649017), ARHGAP5 (rs7151991), and CIITA (rs45601437) in the Asian comparison. PMID: 28100790
    2. ErbB2 modulates gemcitabine and irinotecan/SN-38 chemoresistance of human pancreatic cancer cells via hCNT1 transporter and multidrug-resistance associated protein MRP-2. PMID: 25890497
    3. hCNT1 is a putative determinant for nucleoside analog chemoresistance in ovarian cancer. PMID: 25600708
    4. Human CNT1 fits the profile of a transceptor in a substrate translocation-independent manner and is likely to be relevant to tumor biology. PMID: 23722537
    5. MUC4 and hCNT1 as potential targets to ameliorate the response of pancreatic tumors to gemcitabine treatment. PMID: 22580602
    6. CNT1-mediated gemcitabine uptake shows a higher correlation with the CNT1 expression level than does ENT1-mediated uptake with ENT1 expression. PMID: 22644860
    7. Site-directed mutagenesis analysis reveals that only one sodium binding site is affected by a mutation; lack of serine546 residue is responsible for apparent loss of CNT1 function. PMID: 21998139
    8. our findings identify hCNT1 as a potential candidate to render drug-resistant pancreatic cancer cells amenable to chemotherapy. PMID: 21343396
    9. Two pyrimidine nucleoside analogs were potent inhibitors of CNT1, with negligible transportability and little apparent cytotoxicity, suggesting that they may have utility as cytoprotective agents. PMID: 20854794
    10. variability in hCNT1 and hENT1 expression in tumour and normal human breast tissue with different expression patterns related to patient prognosis and clinical outcome. PMID: 20734919
    11. Patients with low levels of hCNT1 achieved inferior clinical response. PMID: 19647871
    12. hCNT1 and hENT1 are expressed in polarized MDCK cells on the apical and basolateral membrane, respectively, allowing vectorial transport in both directions depending on the relative activity of each transporter for their substrates PMID: 12097333
    13. human pancreatic adenocarcinoma cells overexpress hENT1, although they retain the ability to express a functional hCNT1 transporter, an isoform that confers sensitivity to gemcitabine PMID: 14581375
    14. hCNT1 was by far the isoform whose expression was most frequently reduced or lost in the 3 types of gynecologic tumors analyzed.In uterine cervix tumors, the loss of expression of hCNT1 was significantly associated with the adenocarcinoma subtype. PMID: 15386342
    15. the N4 transport system is a naturally occurring variant of hCNT1, perhaps at the F316 position. Collectively, and G476 is important for correct membrane targeting, folding, and/or intracellular processing of hCNT1 PMID: 15456697
    16. We now report the effects of mutations in transmembrane helix 8 & demonstrate unique S353T- and L354V-induced changes in nucleoside specificity and cation coupling, respectively. PMID: 17279631
    17. In NSCLC and normal tissues expression of hENT1 and hCNT1 ranged from completely negative to high. PMID: 18600541
    18. We found a strong relationship between IHC staining of hCNT3 and clinical resistance to F-ara-A therapy in chronic lymphocytic leukemia. PMID: 18604194
    19. Report expression and hepatobiliary transport characteristics of CNT1 in sandwich-cultured human hepatocytes. PMID: 18635603
    20. In nonsmall cell lung cancer CNT1 565A showed a borderline significant positive influence on the early response for carriers of the variant allele which, however, did not translate to the survival of the patients PMID: 19107936
    21. 5-azacytidine as a novel substrate for hCNT1 and provides direct evidence that hCNT1 is involved in the DNA-demethylating effects of this drug PMID: 19139132
    22. The CNT1 promoter was characterized with respect to DNA response elements and their binding factors are reported. PMID: 19228884

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Concentrative nucleoside transporter (CNT) (TC 2.A.41) family
  • 组织特异性：
    Expressed in kidney.
  • 数据库链接：

    HGNC: 11001

    OMIM: 606207

    KEGG: hsa:9154

    STRING: 9606.ENSP00000286749

    UniGene: Hs.459187