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  4. Recombinant Human Sperm-egg fusion protein Juno (IZUMO1R)

Recombinant Human Sperm-egg fusion protein Juno (IZUMO1R)

  • 货号：
    CSB-YP008789HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP008789HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-MP008789HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    IZUMO1R; FOLR4; JUNO; Sperm-egg fusion protein Juno; Folate receptor 4; Folate receptor delta; FR-delta; IZUMO1 receptor protein JUNO
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    20-250
  • 氨基酸序列
    G DELLNICMNA KHHKRVPSPE DKLYEECIPW KDNACCTLTT SWEAHLDVSP LYNFSLFHCG LLMPGCRKHF IQAICFYECS PNLGPWIQPV GSLGWEVAPS GQGERVVNVP LCQEDCEEWW EDCRMSYTCK SNWRGGWDWS QGKNRCPKGA QCLPFSHYFP TPADLCEKTW SNSFKASPER RNSGRCLQKW FEPAQGNPNV AVARLFASSA PSWELSYTIM VCSLFLPFLS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

We are interest in the following product CSB-BP008789HU.
But we have following questions:
Is there a possibility to order clones or constructs that you use for these proteins?
We are also interested in human Iodothyronine deiodinase Type II and Type III. We would like to have the proteins with selenocysteine residues. However the according to the latest information on your website, the selenocysteines have been mutated instead of using a SECIS element. Could you please let me know if you can generate these proteins without mutations. We are also interested in the clones or constructs.

A:
Very nice to receive your inquiry.
Recombinant Human Sperm-egg fusion protein Juno(IZUMO1R)
CSB-BP008789HU >> Baculovirus
Expression Region: 20-250aa; Full length of the mature protein.
Tag information: Tag type will be determined during the manufacturing process.
The expected tag is N-terminal 6xHis-tagged
Sequence:

GDELLNICMNAKHHKRVPSPEDKLYEECIPWKDNACCTLTTSWEAHLDVSPLYNFSLFHCGLLMPGCRKHFIQAICFYECSPNLGPWIQPVGSLGWEVAPSGQGERVVNVPLCQEDCEEWWEDCRMSYTCKSNWRGGWDWSQGKNRCPKGAQCLPFSHYFPTPADLCEKTWSNSFKASPERRNSGRCLQKWFEPAQGNPNVAVARLFASSAPSWELSYTIMVCSLFLPFLS


1. Sorry, we can't provide the constructs, but the clone is available if you need, pUC vector, lead time is 5-10 working days.
2.The quotations for Human Type II iodothyronine deiodinase(DIO2) (CSB-XP821705HU1 series) and Human Thyroxine 5-deiodinase(DIO3) (CSB-XP345038HU series) were provided to you through email.
The reason why we provided the mutated sequences were also explained in the email. Sorry, we can't provide the original sequence without mutation, and the corresonding clone and contructes can't be provided.The "U" selenocysteine is encoded by UGA and it's normally used as a stop codon in prokaryotic cells, which will lead to early termination of translation, so we will mutate this selenocysteine.

靶点详情

  • 功能：
    Receptor for IZUMO1 present at the cell surface of oocytes (oolemma), which is essential for species-specific gamete recognition and fertilization. The IZUMO1:IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'. Does not bind folate.
  • 基因功能参考文献：
    1. Juno single nucleotide polymorphisms associated with fertilization failure and polyspermy after in vitro fertilization in women. PMID: 29243140
    2. JUNO interacts with IZUMO1 during sperm binding. PMID: 27416963
    3. crystal structures of human IZUMO1, JUNO and the IZUMO1-JUNO complex, establishing the structural basis for the IZUMO1-JUNO-mediated sperm-oocyte interaction PMID: 27309808
    4. crystal structures of human IZUMO1 and JUNO in unbound and bound conformations PMID: 27309818
  • 亚细胞定位：
    Cell membrane; Lipid-anchor, GPI-anchor.
  • 蛋白家族：
    Folate receptor family
  • 数据库链接：

    HGNC: 32565

    OMIM: 615737

    KEGG: hsa:390243

    STRING: 9606.ENSP00000416935

    UniGene: Hs.553758