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  4. Recombinant Human Sperm protamine P1 (PRM1)

Recombinant Human Sperm protamine P1 (PRM1)

  • 中文名称：
    人PRM1重组蛋白
  • 货号：
    CSB-YP018728HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人PRM1重组蛋白
  • 货号：
    CSB-EP018728HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人PRM1重组蛋白
  • 货号：
    CSB-EP018728HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人PRM1重组蛋白
  • 货号：
    CSB-BP018728HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人PRM1重组蛋白
  • 货号：
    CSB-MP018728HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PRM1
  • Uniprot No.：
  • 别名：
    PRM1Sperm protamine P1; Cysteine-rich protamine
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-51
  • 氨基酸序列
    ARYRCCRSQ SRSRYYRQRQ RSRRRRRRSC QTRRRAMRCC RPRYRPRCRR H
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Protamines substitute for histones in the chromatin of sperm during the haploid phase of spermatogenesis. They compact sperm DNA into a highly condensed, stable and inactive complex.
  • 基因功能参考文献：
    1. The expression level of PRM1 was significantly higher in colon cancer tissues and the staining degree of PRM1 in poorly-differentiated was stronger. PMID: 29140788
    2. Bacterial infections have significant negative effects on sperm chromatin condensation and protamine P1/P2 ratio. PMID: 28736810
    3. Protamine-1 and protamine-2 mRNA levels as well as the protamine mRNA ratio and all routine semen parameters revealed significant differences between recurrent miscarriage couples and healthy volunteers. Values of protamine-1 and protamine-2 mRNAs were significantly higher and the protamine mRNA ratio was significantly lower in couples with recurrent miscarriage. PMID: 28854581
    4. examinded the association between protamine gene alleles (PRM1 c.-190C>A, PRM1 c.197G>T, and PRM2 c.248C>T), and YBX2 (c.187T>C and c.1095 + 16A>G) and male infertility. We found that the AA and CA genotypes of the PRM1 c.-190C>A polymorphism had a significant association with infertility (p < 0.001) and the AA genotype was also highly significantly associated with high sperm DNA damage (p < 0.001). PMID: 29227750
    5. The KDM3A to PRM1 mRNA expression ratio can be used as a reliable marker of successful testicular sperm extraction in men with obstructive and non-obstructive azoospermia with 95% sensitivity. PMID: 27027467
    6. Single nucleotide polymorphisms in the human PRM1 gene are associated with a genetic risk factor for idiopathic oligozoospermia. PMID: 27216534
    7. Studies indicate that ribonucleotide reductase M1 polypeptide (RRM1) expression is associated with the response rate and overall survival rate of advanced NSCLC patients treated with gemcitabine-based chemotherapy. PMID: 26092210
    8. The expressed human protamine1 in two fast-growing cell systems, E. coli and HeLa cells. The protamine 1 expression significantly attenuated cell proliferation when compared with control cells. PMID: 25649960
    9. the quantity of PRM1, PRM2, and TNP2 transcripts and the PRM1/PRM2 mRNA ratio affect spermiogenesis, sperm morphology PMID: 25536093
    10. Single nuclotide polymorphisms in PRM1 gene is associated with male infertility. PMID: 22104739
    11. genetic association study in Han population in China: Data suggest that an SNP in PRM1 (rs35576928; T allele; 34R>S) is associated with severe oligozoospermia and can play a protective role against this disease. PMID: 23079002
    12. The SNP of PRM1-190C- > A might be associated with teratozoospermia-induced male infertility in the Han Chinese. PMID: 22574365
    13. significant correlation has been found between protamine 1 and 2 transcripts/proteins and: sperm concentration, progressive sperm motility, sperm fertilization ability as well as embryo quality PMID: 22472940
    14. identified and we report for the first time five novel rare haplotypes encompassing the protamine 1 and 2 genes PMID: 21029114
    15. Data suggest that Casp4, Casp 6 and TNFSF10 are differentially expressed in potentially fertile and subfertile men and represent useful biomarkers for predicting male fertility in combination with P1 and P2. PMID: 21317160
    16. Mutations in the protamine locus may be an infrequent cause of male infertility. PMID: 19602509
    17. single nucleotide polymorphisms in this gene in fertile and infertile males PMID: 12569175
    18. A number of SNPs are present in the testis-specific nuclear protein genes. PMID: 16989827
    19. The P1/P2 ratio has a significant relationship to sperm fertilization ability. PMID: 17011555
    20. Mutations in PRM1 gene were identified in males with oligozoospermia. PMID: 17494104
    21. The PRM1 contributes not only to successful fertilization, but may has an important impact in development of preimplantation embryos. PMID: 18292840
    22. A reduction in contents of PRM1 transcript in spermatozoa may be linked with asthenozoospermia. PMID: 18292846
    23. Decreased pre-P2/P2 and P1/P2 ratios are related to a poor pregnancy outcome, but not with the proportion of embryos obtained after assisted reproduction. PMID: 18314125
    24. Low-frequency protamine 1 gene transversions c.102G->T and c.-107G->C do not correlate with male infertility. PMID: 18381856
    25. Histones localized to the posterior ring region (i.e. the sperm nuclear annulus), whereas PRM1 and PRM2 appeared to be dispersed throughout the entire nucleus. PMID: 18478156
    26. The CCNA1, CCNB1, CCNB2, PRM1, and PRM2 messenger RNA transcript ratios were significantly decreased in patients with spermatogenic disorders. Transcript ratios in patients with successful sperm retrieval were higher than with failed sperm retrieval. PMID: 18692784

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  • 亚细胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    Protamine P1 family
  • 组织特异性：
    Testis.
  • 数据库链接：

    HGNC: 9447

    OMIM: 182880

    KEGG: hsa:5619

    STRING: 9606.ENSP00000310515

    UniGene: Hs.2909