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  4. Recombinant Human Sphingomyelin phosphodiesterase 3 (SMPD3)

Recombinant Human Sphingomyelin phosphodiesterase 3 (SMPD3)

  • 中文名称：
    人SMPD3重组蛋白
  • 货号：
    CSB-YP878920HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人SMPD3重组蛋白
  • 货号：
    CSB-EP878920HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人SMPD3重组蛋白
  • 货号：
    CSB-EP878920HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人SMPD3重组蛋白
  • 货号：
    CSB-BP878920HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人SMPD3重组蛋白
  • 货号：
    CSB-MP878920HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SMPD3
  • Uniprot No.：
  • 别名：
    Cca1; Confluent 3Y1 cell-associated protein 1; Neutral sphingomyelinase 2; Neutral sphingomyelinase II; NSMA2_HUMAN; nSMase-2; nSMase2; SMPD3; Sphingomyelin phosphodiesterase 3; sphingomyelin phosphodiesterase 3, neutral membrane (neutral sphingomyelinase II)
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-655
  • 氨基酸序列
    MVLYTTPFPN SCLSALHCVS WALIFPCYWL VDRLAASFIP TTYEKRQRAD DPCCLQLLCT ALFTPIYLAL LVASLPFAFL GFLFWSPLQS ARRPYIYSRL EDKGLAGGAA LLSEWKGTGP GKSFCFATAN VCLLPDSLAR VNNLFNTQAR AKEIGQRIRN GAARPQIKIY IDSPTNTSIS AASFSSLVSP QGGDGVARAV PGSIKRTASV EYKGDGGRHP GDEAANGPAS GDPVDSSSPE DACIVRIGGE EGGRPPEADD PVPGGQARNG AGGGPRGQTP NHNQQDGDSG SLGSPSASRE SLVKGRAGPD TSASGEPGAN SKLLYKASVV KKAAARRRRH PDEAFDHEVS AFFPANLDFL CLQEVFDKRA ATKLKEQLHG YFEYILYDVG VYGCQGCCSF KCLNSGLLFA SRYPIMDVAY HCYPNKCNDD ALASKGALFL KVQVGSTPQD QRIVGYIACT HLHAPQEDSA IRCGQLDLLQ DWLADFRKST SSSSAANPEE LVAFDVVCGD FNFDNCSSDD KLEQQHSLFT HYRDPCRLGP GEEKPWAIGT LLDTNGLYDE DVCTPDNLQK VLESEEGRRE YLAFPTSKSS GQKGRKELLK GNGRRIDYML HAEEGLCPDW KAEVEEFSFI TQLSGLTDHL PVAMRLMVSS GEEEA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. Regulates the cell cycle by acting as a growth suppressor in confluent cells. Probably acts as a regulator of postnatal development and participates in bone and dentin mineralization. Binds to anionic phospholipids (APLs) such as phosphatidylserine (PS) and phosphatidic acid (PA) that modulate enzymatic activity and subcellular location. May be involved in IL-1-beta-induced JNK activation in hepatocytes. May act as a mediator in transcriptional regulation of NOS2/iNOS via the NF-kappa-B activation under inflammatory conditions.
  • 基因功能参考文献：
    1. the DK switch regulates ceramide generation by nSMase2 and is governed by an allosteric interdomain interaction at the membrane interface PMID: 28652336
    2. The enzymes involved in sphingolipid metabolism are expressed abnormally in B cells from SLE patients. TLR signaling induced the abnormal expression of sphingomyelin phosphodiesterase 3 (SMPD3). TLR signaling also induced the transport of SMPD3 from the Golgi apparatus. Furthermore, the dysfunction of SMPD3 enhanced TLR-induced inflammatory response of B cells and macrophages in turn. PMID: 28889482
    3. ATRA regulates nSMase2 transcriptionally through the retinoic acid receptor-alpha, but this is independent of previously identified transcriptional regulators of nSMase2 (Sp1, Sp3, Runx2) and is not through increased promoter activity. PMID: 27013100
    4. Overexpression of Smpd3 induced cytodifferentiation of HPDL cells, which could be suppressed by an inhibitor of its protein product, nSMase2. In addition,Smpd3 harboring a SNP (rs145616324) showed no activity and failed to induce cytodifferentiation of HPDL cells. Together, these findings suggest that Smpd3 plays an important role in the osteoblastic differentiation of HPDL cells. PMID: 28221099
    5. low oxLDL concentration triggers sprouting angiogenesis that involves ROS-induced activation of the neutral sphingomyelinase-2/sphingosine kinase-1 pathway, and is effectively inhibited by GW4869. PMID: 26855418
    6. nSMase2 is a novel p53 target gene, regulated by the DNA damage pathway to induce cell growth arrest. PMID: 26512957
    7. These results suggest that OTC is a potent stimulant of nSMase-2 expression and that there may be unanticipated complications of OTC supplementation. PMID: 25047167
    8. nSMase2 involvement in cellular processes including inflammatory signaling, exosome generation, cell growth, and apoptosis, which in turn play important roles in pathologies such as cancer metastasis, Alzheimer's disease PMID: 25465297
    9. SMPD3 plays an important role in the release of microRNAs into extracellular spaces. PMID: 25394686
    10. The data shows that nSMase3 acts as a signaling nSMase in skeletal muscle that is essential for TNF-stimulated oxidant activity. PMID: 25180167
    11. This is the first report on the critical role of ceramide generated by nSMase2 in stem cell ciliogenesis and differentiation. PMID: 24694597
    12. We found upregulation of specific sphingolipid enzymes, namely sphingomyelin synthase 1 (SMS1), sphingomyelinase 3 (SMPD3), and glucosylceramide synthase (GCS) in the endometrium of endometriotic women. PMID: 24960545
    13. The H2O2-induced src/PDGFRbeta/SK1 signaling cascade was impaired in nSMase2-deficient fro/fro cells and was rescued by exogenous C2Cer that activated src/PDGFRbeta/SK1. PMID: 23651497
    14. a requirement for nSMase2-mediated cancer cell exosomal miRNAs in the regulation of metastasis through the induction of angiogenesis in inoculated tumors. PMID: 23439645
    15. MMP2 and neutral sphingomyelinase-2 play a role in vasculopathy triggered by a humoral immune response in transplants. PMID: 22082680
    16. NSMase-2- and PP2A-dependent regulation of IRAK-1 degradation is a novel mechanism to fine tune the magnitude of IL-1beta response. PMID: 21708940
    17. Neutral sphingomyelinase 2 (nSMase2) is the primary neutral sphingomyelinase isoform activated by tumour necrosis factor-alpha. PMID: 21303347
    18. nSMase2 is a major component of ATRA-induced growth arrest of MCF-7 cells; S6K is a novel downstream target of nSMase2 PMID: 21536668
    19. Neutral sphingomyelinase 2 (nSMase2) is a phosphoprotein regulated by calcineurin (PP2B) PMID: 20106976
    20. Data show DA remarkably increased the NSMase2 message and protein, whereas little change in NSMase1 and NSMase3 mRNAs. PMID: 19698806
    21. Upon urokinase plasminogen activator stimulation, UPAR, Matrix metalloproteinases, integrin alphavbeta3 and NSMASE2 form a signaling complex that take part in mitogenic signaling in ECV304 cells. PMID: 19735728
    22. nSMase2 is a structural gene for nSMase, acts as a bona fide N-SMase in cells, and has a role in the regulation of cell growth and cell signaling PMID: 12566438
    23. The metabolic and chemical characteristics of SMPD3 are reported. PMID: 16517606
    24. Here, we show that this nSMase2 is up-regulated by an oxidant (H(2)O(2)) and is inhibited by an antioxidant (glutathione (GSH)). PMID: 16631623
    25. p38 MAPK is an upstream regulator of nSMase2 and there is a role for nSMase2 in pro-inflammatory responses induced by TNF-alpha as a regulator of adhesion proteins. PMID: 17085432
    26. These results indicate the presence of NSMASE2 in the inner leaflet of plasma membrane. PMID: 17349629
    27. Nucleotide sequencing of the highly conserved SMPD3 gene in a large panel of human cancers revealed mutations in 5 (5%) of 92 acute myeloid leukemias (AMLs) and 8 (6%) of 131 acute lymphoid leukemias (ALLs), but not in other tumor types PMID: 18299447

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  • 亚细胞定位：
    Golgi apparatus membrane; Lipid-anchor. Cell membrane; Lipid-anchor. Note=May localize to detergent-resistant subdomains of Golgi membranes of hypothalamic neurosecretory neurons (PubMed:10823942). Localizes to plasma membrane in confluent contact-inhaibited cells (PubMed:15051724).
  • 蛋白家族：
    Neutral sphingomyelinase family
  • 组织特异性：
    Predominantly expressed in brain.
  • 数据库链接：

    HGNC: 14240

    OMIM: 605777

    KEGG: hsa:55512

    STRING: 9606.ENSP00000219334

    UniGene: Hs.368421