Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Human Steroid receptor RNA activator 1 (SRA1)

Recombinant Human Steroid receptor RNA activator 1 (SRA1)

  • 货号：
    CSB-YP022648HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP022648HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP022648HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP022648HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP022648HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SRA1
  • Uniprot No.：
  • 别名：
    MGC87674; PP7684; SR A1; SRA; SRA1; SRA1_HUMAN; SRAP; Steroid receptor coactivator; Steroid receptor RNA activator 1 (complexes with NCOA1); Steroid receptor RNA activator 1; Steroid receptor RNA activator protein; STRAA1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-236
  • 氨基酸序列
    MTRCPAGQAE VEMAELYVKP GNKERGWNDP PQFSYGLQTQ AGGPRRSLLT KRVAAPQDGS PRVPASETSP GPPPMGPPPP SSKAPRSPPV GSGPASGVEP TSFPVESEAV MEDVLRPLEQ ALEDCRGHTR KQVCDDISRR LALLQEQWAG GKLSIPVKKR MALLVQELSS HRWDAADDIH RSLMVDHVTE VSQWMVGVKR LIAEKRSLFS EEAANEEKSA ATAEKNHTIP GFQQAS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Functional RNA which acts as a transcriptional coactivator that selectively enhances steroid receptor-mediated transactivation ligand-independently through a mechanism involving the modulating N-terminal domain (AF-1) of steroid receptors. Also mediates transcriptional coactivation of steroid receptors ligand-dependently through the steroid-binding domain (AF-2). Enhances cellular proliferation and differentiation and promotes apoptosis in vivo. May play a role in tumorigenesis.
  • 基因功能参考文献：
    1. our findings demonstrated that lncRNA SRA is highly correlated with cancer progression and cervical cancer cell proliferation and migration. Furthermore, these results indicate that lncRNA SRA may be a potential therapeutic target and prognostic marker for cervical malignancy. PMID: 29039612
    2. Surprisingly, Rac1 is not located at the binding site on the Sra1 subunit of the WAVE regulatory complex previously identified by mutagenesis and biochemical data. Rather, it binds to a distinct, conserved site on the opposite end of Sra1. PMID: 28949297
    3. Steroid receptor RNA activator 1-small interfering RNA treatment significantly increased ER-alpha levels but reduced ER-beta levels in endometriotic stromal cells (ESCs). PMID: 27694140
    4. These findings suggest that SRA genetic variants may contribute to breast cancer risk PMID: 26967566
    5. SRA1 was down expressed in HCC and its level was associated with tumor size and GLU level in patients with HCC for the first time. SRA1 may be a helpful biomarker to diagnostic HCC. PMID: 28085012
    6. By studying a cohort of idiopathic hypogonadotropic hypogonadism, our findings strongly suggest that SRA1 gene function is required for initiation of puberty in humans PMID: 27086651
    7. Results identified H15-H18 domains in SRA gene playing a key role in regulating ER-mediated transcription. PMID: 27406387
    8. REVIEW: Biologic function and role in disease PMID: 27282881
    9. in human pluripotent stem cells SRA interacts with NANOG and is important for maintenance of the pluripotent state. PMID: 26496121
    10. Data suggest that expression of SRA1 (mRNA and protein) is linked to cancer cell motility (invasiveness of breast/cervical tumor cells) and regulation of gene expression. PMID: 26581859
    11. lncRNA SRA expression is potentially associated with PCOS and it has positive correlation with obesity in PCOS, thereby suggesting that elevated lncRNA SRA might be an important mediator in adiposity-related processes in PCOS for susceptible individuals. PMID: 25609053
    12. In endometrioma patients, the surrounding ovarian tissue steroid receptor RNA activator expression is higher compared to controls. PMID: 23749764
    13. The structure is a five-helix bundle that is distinct from known RNA-binding motifs and instead is similar to the carboxy-terminal domain of the yeast spliceosome protein PRP18, which stabilizes specific protein-protein interactions. PMID: 24486609
    14. NMR spectra of SRA1p in the presence of its 80-nt RNA target indicate that this protein must be part of a multi-protein complex in order to recognize its proposed RNA recognition element. PMID: 24486611
    15. This study provides evidence that both ER-beta1 and SRAP could be predictive biomarkers of tamoxifen benefit in ER-alpha-negative premenopausal early breast cancer. PMID: 23579816
    16. The presence of SRAP and multiple SRAP-like peptides in estrogen receptor positive breast cancer samples correlates with clinical outcome. PMID: 23907597
    17. results show that the NTD of ERalpha and AR contains a novel RBM that directly binds SRA, and that STR5 can serve as a novel class of RNA inhibitor of ERalpha and AR signaling by interfering with Pus1p-mediated SRA pseudouridylation PMID: 22998747
    18. A report of the first experimentally derived secondary structure of a human lncRNA, the steroid receptor RNA activator. PMID: 22362738
    19. A significant (Student's t-test, P < 0.003) higher SRA-intron-1 relative expression is reported in breast tumors with higher progesterone receptor contents. PMID: 19483093
    20. Alternative splicing of intron-1 is used by breast cancer cells to regulate the balance between coding and functional noncoding SRA1 RNA. PMID: 16848684
    21. SRA noncoding RNA enhances myogenic differentiation and myogenic conversion of non-muscle cells through the co-activation of MyoD activity, but SRAP prevented this SRA RNA-dependant co-activation. PMID: 20855289
    22. DEAD-box RNA helicase p68 (DDX5) and its associated noncoding RNA, steroid receptor RNA activator (SRA), form a complex with CTCF that is essential for insulator function PMID: 20966046
    23. The data suggest unanticipated roles for SRA in glucose uptake, cellular signaling, T(3) hormone generation, and invasion/metastasis. PMID: 20219889
    24. in breast cancer patients, SRAP levels were higher in estrogen receptor-alpha positive, in progesterone receptor positive & in older patients; data suggest SRAP levels may provide additional information on prognosis in a specific set of patients PMID: 19740422
    25. Both transcript and protein products of the SRA1 gene co-modulate the transcriptional activity of steroid receptors. PMID: 20153324
    26. Chlamydial protease CT441 interacts with SRAP1 co-activator of estrogen receptor alpha and partially alleviates its co-activation activity. PMID: 20079837
    27. Reduction of coactivator expression by antisense oligodeoxynucleotides inhibits ERalpha transcriptional activity and MCF-7 proliferation PMID: 11818499
    28. Results identify three new steroid receptor RNA activator (SRA-RNA) isoforms that likely encode stable proteins, and that are widely expressed in breast cancer cell lines. PMID: 12565891
    29. 2.0A crystal structure of the human PXR ligand-binding domain (LBD) in complex with the cholesterol-lowering compound SR12813 and a 25 amino acid residue fragment of the human steroid receptor coactivator-1 (SRC-1) containing one LXXLL motif PMID: 12909012
    30. SRA potentiates the estrogen-induced transcriptional activity of both ERalpha and ERbeta. PMID: 12943696
    31. SRA is an ERalpha AF-1-specific coactivator that enhances the agonist activity of tamoxifen-bound ERalpha and may contribute to tamoxifen resistance PMID: 15351741
    32. Upregulation of steroid receptor RNA activator is associated with serous Cystadenocarcinoma of the ovary PMID: 15607539
    33. data suggest that the presence of both coding SRA RNA and its corresponding SRAP modifies the activity of estrogen receptor in breast cancer cells and that SRAP could be a new clinical marker for breast cancer PMID: 16152589
    34. analysis of of a complex formed by the SRC1 interaction domain (SID) of CBP and the activation domain (AD1) of SRC1 PMID: 16540468
    35. alternative splicing of intron-1 is one mechanism used by breast cancer cells to regulate the balance between coding and functional noncoding SRA1 RNAs PMID: 16848684
    36. STAT3, Runx2, and steroid receptor coactivator-1 are critical molecules in mediating leptin-stimulated cell osteogenesis in TOLF (thoracic ossification of ligament flavum) PMID: 17702747
    37. At least three genes, orthologous to loci in this LD block, HBEGF, IK, and SRA1, result independently in a phenotype of myocardial contractile dysfunction when their expression is reduced with morpholino antisense reagents. PMID: 19064678
    38. These findings reveal novel functions of SRA and Dax-1 in steroidogenesis and adrenal biology. PMID: 19188450

    显示更多

    收起更多

  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    SRA1 family
  • 组织特异性：
    Highly expressed in liver and skeletal muscle and to a lesser extent in brain. Also expressed in both normal and tumorigenic breast epithelial cell lines. Significantly up-regulated in human tumors of the breast, ovary, and uterus.
  • 数据库链接：

    HGNC: 11281

    OMIM: 603819

    KEGG: hsa:10011

    STRING: 9606.ENSP00000337513

    UniGene: Hs.653135