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  4. Recombinant Human Sterol O-acyltransferase 2 (SOAT2), partial

Recombinant Human Sterol O-acyltransferase 2 (SOAT2), partial

  • 货号：
    CSB-YP022386HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP022386HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP022386HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP022386HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP022386HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    SOAT2; ACACT2; ACAT2; Sterol O-acyltransferase 2; EC 2.3.1.26; Acyl-coenzyme A:cholesterol acyltransferase 2; ACAT-2; Cholesterol acyltransferase 2
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the formation of fatty acid-cholesterol esters, which are less soluble in membranes than cholesterol. Plays a role in lipoprotein assembly and dietary cholesterol absorption. Utilizes oleoyl-CoA ((9Z)-octadecenoyl-CoA) and linolenoyl-CoA ((9Z,12Z,15Z)-octadecatrienoyl-CoA) as substrates. May provide cholesteryl esters for lipoprotein secretion from hepatocytes and intestinal mucosa.; Has lower enzymatic activity compared to isoform 1.; Has lower enzymatic activity compared to isoform 1.
  • 基因功能参考文献：
    1. Describe a gender-related difference in hepatic ACAT2 activity in normolipidemic non-obese Chinese patients suggesting a possible role for ACAT2 in the regulation of cholesterol metabolism in humans. PMID: 19467657
    2. ACAT-2 expression was negative in clear cell type renal cell carcinoma and normal kidney. PMID: 18269457
    3. ACAT2 expression was induced upon R1881 (androgen agonist) treatment in prostate cancer cells PMID: 18000807
    4. HNF4alpha positively regulates ACAT2 gene expression at mRNA level. Overexpression of HNF4alpha increased ACAT2 expression, whereas knockdown of HNF4alpha decreased ACAT2 expression. PMID: 22155889
    5. results show that the enzymatic activity of mutant Glu(14)Gly is approximately two times higher than wildtype, and that this increase is primarily due to the increased expression and/or stability of the mutant ACAT2 protein PMID: 25917363
    6. Low ACAT2 expression is associated with clear cell renal cell carcinoma. PMID: 26715271
    7. data demonstrate that the ACAT2 expression of human leukocytes is responsible for the excretion of lipoproteins containing cholesteryl/steryl esters (CE/SE), and suggest that the excretion of lipoproteins containing the ACAT2-catalyzed CS/SE may avoid cytotoxicity PMID: 27688150
    8. Lipid-induced stabilization of ACAT2 ameliorates lipotoxicity from excessive cholesterol and fatty acid. Cysteine ubiquitylation of ACAT2 constitutes an important mechanism for sensing lipid-overload-induced ROS and fine-tuning lipid homeostasis. PMID: 28604676
    9. human ACAT2 is transcriptionally regulated by cholesterol. PMID: 17950700
    10. TG-interacting factor 1 (Tgif1) is an important repressor of SOAT2 gene expression. PMID: 24478032
    11. CDX2, a known positive regulator of hepatocyte differentiation, was regulated by miR-181d and directly activated SOAT2 gene expression. PMID: 24103759
    12. We found a new single-nucleotide polymorphism (SNP; a point mutation in intron 1, IVS1 -8 G-->C) in the ACAT-2 gene. Our data suggest that the ACAT-2 gene may not affect lipid levels in humans. PMID: 12621162
    13. Fully differentiated macrophages express ACAT2 in addition to ACAT1 under various pathologic conditions. PMID: 14615411
    14. ACAT-1 transcripts predominate in human liver and ACAT-2 transcripts predominate in human duodenum and support the notion that ACAT-2 has an important regulatory role in liver and intestine. PMID: 14729857
    15. increasing DGAT1, ACAT1, or ACAT2 expression stimulates the assembly and secretion of VLDL from liver cells PMID: 15308631
    16. ACAT2 provided the major cholesterol-esterifying activity in 3 of 4 human liver samples. PMID: 15451793
    17. transcription factors hepatic nuclear factor 1 (HNF1)alpha and beta play an important part in the regulation of the ACAT2 promoter PMID: 15961790
    18. The structural features of various sterols as substrates and/or activators of ACAT1 and ACAT2 in vitro are reported. PMID: 15992359
    19. Serum-induced depletion of cellular cholesterol available for esterification by ACAT was a strong, independent predictor of major adverse cardiovascular events and death. PMID: 16230498
    20. Elevated ACAT2 expression may serve as a new biomarker for certain form(s) of hepatocellular carcinoma. PMID: 16274362
    21. Alternative splicing produces two human ACAT2 mRNA variants that encode the novel ACAT2 isoenzymes. Our findings might help to understand the regulation of the ACAT2 gene expression under certain physiological and pathological conditions. PMID: 16331323
    22. histidine residues located at the active site are very crucial both for the catalytic activity of the enzyme and for distinguishing ACAT1 from ACAT2 with respect to enzyme catalysis and substrate specificity PMID: 16647063
    23. We resequenced 4 candidate genes for HDL regulation and identified several functional nonsynonymous mutations including 4 in lecithin:cholesterol acyltransferase (LCAT), leaving 88% (110/124) of HDL deficient subjects without a genetic diagnosis. PMID: 17303779

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Membrane-bound acyltransferase family, Sterol o-acyltransferase subfamily
  • 组织特异性：
    Expression seems confined in hepatocytes and enterocytes.
  • 数据库链接：

    HGNC: 11178

    OMIM: 601311

    KEGG: hsa:8435

    STRING: 9606.ENSP00000301466

    UniGene: Hs.656544