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  4. Recombinant Human Stromal interaction molecule 2 (STIM2), partial

Recombinant Human Stromal interaction molecule 2 (STIM2), partial

  • 货号：
    CSB-YP022830HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP022830HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP022830HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP022830HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP022830HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    FLJ39527; KIAA1482; STIM 2; STIM2; STIM2_HUMAN; Stromal interaction molecule 2
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Functions as a highly sensitive Ca(2+) sensor in the endoplasmic reticulum which activates both store-operated and store-independent Ca(2+)-influx. Regulates basal cytosolic and endoplasmic reticulum Ca(2+) concentrations. Upon mild variations of the endoplasmic reticulum Ca(2+) concentration, translocates from the endoplasmic reticulum to the plasma membrane where it probably activates the Ca(2+) release-activated Ca(2+) (CRAC) channels ORAI1, ORAI2 and ORAI3. May inhibit STIM1-mediated Ca(2+) influx.
  • 基因功能参考文献：
    1. Expression of full-length STIM1 with STIM2 in a 5:1 ratio causes suppression of sustained agonist-induced Ca(2+) oscillations and protects cells from Ca(2+) overload. PMID: 29581306
    2. Stim2 deletion reduced SOCE by more than 90% in NIH 3T3 cells and abolishes STIM1 translocation to plasma membrane-associated ER membrane junctions. PMID: 29783744
    3. With STIM2 being essential for life, but apparently not for development, newly available data demonstrate a complex and still intriguing behaviour that this review summarizes, updating current knowledge of STIM2 function [review] PMID: 28087881
    4. The STIM2 protein play important roles in TGF-beta-induced EMT and these effects are related to both store-operated calcium entry and non-store-operated calcium entry. PMID: 28479254
    5. What is less clear is how the spatial and temporal spread of intracellular Ca(2+) is shaped and regulated by differential expression of the individual SOCE genes and their splice variants, their heteromeric combinations and pre- and posttranslational modifications. This review focuses on principle mechanisms regulating expression, splicing, and targeting of Ca(2+) release-activated Ca(2+) (CRAC) channels. PMID: 26911279
    6. While STIM1 is a full Orai1-agonist, leucine-replacement of this crucial residue in STIM2 endows it with partial agonist properties, which may be critical for limiting Orai1 activation stemming from its enhanced sensitivity to store-depletion PMID: 24492416
    7. These results imply that the splice variant STIM2.1 is an additional player tuning Orai1 activation in vivo. PMID: 25896806
    8. STIM2.1 cannot activate Orai1 due to splicing in residues within the channel-activating domain (CAD). STIM2.1 confers a calcium dependent dominant-negative function on both STIM1 and STIM2 (STIM2.2). Affinity of the STIM2.1 calmodulin binding site within the CAD domain is increased compared to STIM2.2. PMID: 25896806
    9. STIM2 enhances receptor-stimulated Ca(2) signaling by promoting recruitment of STIM1 to the endoplasmic reticulum-plasma membrane junctions. PMID: 25587190
    10. STIM2beta does not by itself strongly bind Orai1, it is recruited to Orai1 channels by forming heterodimers with other STIM isoforms. PMID: 26033257
    11. Imin channels are regulated by STIM2, TRPC3-containing INS channels are induced by STIM1, and TRPC1-composed Imax channels are activated by both STIM1 and STIM2. PMID: 25533457
    12. The higher amplitude of store-operated Ca2+ entry was associated to the over-expression for Stim2, Orai2-3, and TRPC1 while Stim2 levels remained constant and Stim1, Orai1, Orai3, TRPC1 and TRPC4 proteins were over-expressed in primary myelofibrosis. PMID: 24603752
    13. the reciprocal shift in transient receptor potential channel 1 (TRPC1) and stromal interaction molecule 2 (STIM2) contributes to Ca2+ remodeling and cancer hallmarks in colorectal carcinoma cells PMID: 25143380
    14. STIM2 is highly expressed and controls store-operated Ca(2+) entry in human melanoma. The invasive and migratory potential of melanoma cells was reduced upon silencing of STIM2. PMID: 24472175
    15. Binding of the lysine rich domains of STIM1 and STIM2 to phosphatidylinositol 4,5 biphosphate rich liposomes depends on their oligomerization. PMID: 24044355
    16. STIM2 is involved in spatial learning and plays an important role in ischemic-induced neuronal cell death during brain hypoxia. The absence of STIM2 reduces life expectancy in mice by sudden death. PMID: 198439
    17. STIM2 regulates store-operated Ca2+ entry as a sensor of mild reductions in ER calcium levels. PMID: 23359669
    18. The role of STIM2 in store operated calcium entry and its relevance in cellular physiology. [review] PMID: 22477146
    19. Studies indicate that in vertebrates, stromal interaction molecule proteins STIM1 and STIM2 are expressed ubiquitously PMID: 22914293
    20. It was shown that polyamines regulate intestinal epithelial restitution through TRPC1-mediated Ca2+ signaling by altering the ratio of STIM1 to STIM2. Overexpression of ornithine decarboxylase resulted in increased STIM1 but decreased STIM2 expression. PMID: 22592407
    21. Stromal interaction molecule 2 is frequently overexpressed in colorectal tumors and confers a tumor cell growth suppressor phenotype. PMID: 22125164
    22. Stim1 and Stim2 were increased in brains of chronic epileptic rodents and strongly expressed in hippocampal specimens from medial temporal lobe epilepsy patients. PMID: 21906591
    23. STIM1 and STIM2 are located in the acidic Ca2+ stores and associates with Orai1 upon depletion of the acidic stores in human platelets. PMID: 21321120
    24. a new model for STIM2-mediated regulation of ORAI1 in which two distinct proteins, STIM2 and preSTIM2, control store-dependent and store-independent modes of ORAI1 activation. PMID: 21383014
    25. Data suggest that divergent Ca(2+)- and SAM-dependent stabilization of the EF-SAM fold contributes to the disparate regulation of store-operated Ca(2+) entry by STIM1 and STIM2. PMID: 21217057
    26. involved in both store-operated calcium entry and cell proliferation PMID: 20172609
    27. STIM2 is a necessary partner of STIM1 for excitation-contraction coupling. PMID: 20436167
    28. STIM proteins function as Ca(2+) store sensors in the signaling pathway connecting Ca(2+) store depletion to Ca(2+) influx. PMID: 16005298
    29. STIM2 is an inhibitor of store-operated channel (SOC) activation and plays a coordinated role with STIM1 in controlling SOC-mediated Ca(2+) entry signals. PMID: 16860747
    30. STIM2 has two modes of activating CRAC channels: a store-operated mode activated through depletion of ER Ca2+ stores by inositol 1,4,5-trisphosphate and store-independent activation mediated by cell dialysis; both regulated by calmodulin. PMID: 17905723
    31. Study places STIM2 at the center of a feedback module that keeps basal cytosolic and endoplasmic reticulum Ca2+ concentrations within tight limits. PMID: 18160041
    32. Our biophysical studies reveal a structural stability difference in the EF-SAM region between STIM1 and STIM2, which may account for their different biological functions. PMID: 18166150
    33. distinct oligomerization dynamics of STIM isoforms have evolved to adapt to differential roles in Ca(2+) homeostasis and signaling PMID: 19019825
    34. Data demonstrate that cellular levels of STIM1 and STIM2, which are key players in capacitative Ca2+ entry, depend on presenilins. PMID: 19111578
    35. results provide evidence for a role of STIM1 protein in the control of Ca2+ influx in neutrophils excluding a STIM2 involvement in this process PMID: 19433064
    36. Results show that the 55-amino-acid STIM2 terminus substituted within STIM1 strikingly enhances both Orai1-mediated Ca(2+) entry and constitutive coupling to activate Orai1 channels. PMID: 19487696
    37. Translation initiation from a non-AUG (UUG) start site. PMID: 11463338

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Single-pass type I membrane protein. Note=Dynamically translocates from a uniform endoplasmic reticulum distribution to punctual endoplasmic reticulum-plasma membrane junctions in response to decrease in endoplasmic reticulum Ca(2+) concentration.
  • 组织特异性：
    Expressed in all tissues and tumor cell lines examined.
  • 数据库链接：

    HGNC: 19205

    OMIM: 610841

    KEGG: hsa:57620

    STRING: 9606.ENSP00000417569

    UniGene: Hs.744950