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  4. Recombinant Human Syncytin-1 (ERVW-1), partial

Recombinant Human Syncytin-1 (ERVW-1), partial

  • 货号：
    CSB-YP891578HU
  • 规格：
    ￥2208
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    ERVW-1
  • Uniprot No.：
  • 别名：
    ERVW-1; ERVWE1; Syncytin-1; Endogenous retrovirus group W member 1; Env-W; Envelope polyprotein gPr73; Enverin; HERV-7q Envelope protein; HERV-W envelope protein; HERV-W_7q21.2 provirus ancestral Env polyprotein; Syncytin) [Cleaved into: Surface protein; SU; gp50); Transmembrane protein; TM; gp24)]
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 来源：
    Yeast
  • 分子量：
    49.0 kDa
  • 表达区域：
    21-443aa
  • 氨基酸序列
    APPPCRCMTSSSPYQEFLWRMQRPGNIDAPSYRSLSKGTPTFTAHTHMPRNCYHSATLCMHANTHYWTGKMINPSCPGGLGVTVCWTYFTQTGMSDGGGVQDQAREKHVKEVISQLTRVHGTSSPYKGLDLSKLHETLRTHTRLVSLFNTTLTGLHEVSAQNPTNCWICLPLNFRPYVSIPVPEQWNNFSTEINTTSVLVGPLVSNLEITHTSNLTCVKFSNTTYTTNSQCIRWVTPPTQIVCLPSGIFFVCGTSAYRCLNGSSESMCFLSFLVPPMTIYTEQDLYSYVISKPRNKRVPILPFVIGAGVLGALGTGIGGITTSTQFYYKLSQELNGDMERVADSLVTLQDQLNSLAAVVLQNRRALDLLTAERGGTCLFLGEECCYYVNQSGIVTEKVKEIRDRIQRRAEELRNTGPWGLLSQ
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    This endogenous retroviral envelope protein has retained its original fusogenic properties and participates in trophoblast fusion and the formation of a syncytium during placenta morphogenesis. May induce fusion through binding of SLC1A4 and SLC1A5.; Endogenous envelope proteins may have kept, lost or modified their original function during evolution. Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. The surface protein (SU) mediates receptor recognition, while the transmembrane protein (TM) acts as a class I viral fusion protein. The protein may have at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of membranes.
  • 基因功能参考文献：
    1. These data revealed that human endogenous retrovirus W env might contribute to increase nitric oxide production and microglial migration ability in neuropsychological disorders by regulating the expression of inducible nitric oxide synthase. PMID: 28656540
    2. CD47 seems to mediate fusion mostly through broad contact surfaces between the partners' cell membrane while syncytin-1 mediate fusion through phagocytic-cup like structure. PMID: 27714815
    3. HERV-W was expressed in demyelinated lesions from multiple sclerosis brains, which were all positive for this endogenous pathogenic protein. Pronounced HERV-W immunoreactivity in active multiple sclerosis lesions was intimately associated with areas of active demyelination throughout the successive stages of lesion evolution in multiple sclerosis brains. PMID: 27456869
    4. ERVWE1, ERVFRDE1 and ERV3 transcription was down-regulated in hydatidiform moles and gestational trophoblastic neoplasia. PMID: 26992684
    5. Study found no associations between embroynal ERVW-1 polymorphisms and preeclampsia. PMID: 27546366
    6. Identification of the two CpG dinucleotides around transcription start site as key epigenetic elements has provided valuable information for further studies on the epigenetic regulation of syncytin-1 in pancreatic cancer cells PMID: 26230721
    7. Positive associations were observed between HERV-w methylation and dietary intakes of beta-carotene and carotenoids in a population of obese subjects. PMID: 25340371
    8. Syncytin-1 and ASCT-2 is localized at the acrosomal region and equatorial segment of the spermatozoa. PMID: 24687878
    9. Data show that Multiple Sclerosis-Associated Retrovirus (MSRV) is predominantly expressed over ERWVE1 in astrocyte-derived U-87 glioblastoma cell line. PMID: 24814867
    10. The effect of ETS1 on syncytialization of trophoblasts likely results, at least in part, from inhibition of syncytin expression. PMID: 25651508
    11. These results indicated that aberrant hypermethylation is involved in downregulation of syncytin-1, and epigenetic alterations may play a significant role in the development of preeclampsia PMID: 23888950
    12. Exosomes from cultured primary villous cytotrophoblasts and sera of pregnant women have syncytin-1 on their surfaces. It acts on cellular binding and uptake of exosomes. PMID: 24812088
    13. Increased trophoblast apoptosis and altered expression levels of syncytin-1, calpain 1, and AIF is observed in preeclamptic placentas. PMID: 24413738
    14. Syncytin-1 overexpression may be an indicator of urothelial cell carcinoma risk. PMID: 24013223
    15. Insertionally polymorphic HERV-K113 and HERV-K115 are not associated with HIV infections. PMID: 24204983
    16. Influenza A virus infection transactivated ERVWE1 by increasing the transcription of GCM1. PMID: 24478419
    17. Overexpression of HERV-W envelope gene elevated the levels of SK3 channel in neuroblastoma cells. PMID: 23727510
    18. Syncytin-1 knockdown significantly inhibited BeWo cell growth and DNA synthesis and Syncytin-1 could promote the G1/S transition through p15 and CDK4. PMID: 23333240
    19. In discordant monozygotic twins, HERVWE1 expression is higher in smaller fetuses and lower in larger counterparts. Methylation of the HERVWE1 gene promoter region may participate in gene regulation. PMID: 23290063
    20. This study linked decreased Syncytin-1 expression to an epigenetic hypermethylation of the entire promoter of ERVW-1. PMID: 23457515
    21. HERV-W env transcription was found to be elevated in bipolar disorder and schizophrenia subjects compared to controls. Molecular sequence characteristics may represent distinct features of the genome of patients with bipolar disorder and schizophrenia. PMID: 23212585
    22. Corticotropin-releasing hormone did not influence the differentiation of isolated trophoblasts into functional syncytium as determined by beta-hCG secretion, albeit inducing syncytin-1 expression. PMID: 22971074
    23. Data suggest that sorting of syncytin-1 into placental exosomes represents mechanism allowing syncytin-1 to reach and interact with target cells of maternal immune system to execute its immune-modulatory roles during pregnancy. PMID: 22999499
    24. proposed that Syncytin-1 is regulated by PPARgamma/RXRalpha signaling during placentogenesis to drive multinuclear syncytiotrophoblast layer formation and maintenance. PMID: 22573555
    25. The role of syncytin-1 and Pb1 in cell-cell fusion was studied using lentiviral vectors that express short hairpin RNAs for stable knockdown of both genes. PMID: 22573740
    26. The findings suggested that syncytin 1 is shed from the placenta into the maternal circulation in association with placental microvesicles, and modulates immune cell activation and the responses of immune cells to lipopolysaccharide stimulation. PMID: 22348442
    27. Data show that beta-catenin/BCL9-Like (BCL9L)/T-cell factor 4 (TCF4) signalling directly targets the GCM1/syncytin pathway and thereby regulates the fusion of human choriocarcinoma cells. PMID: 22109522
    28. results further highlighted the existence of a correlation between the extent of the decrease in the expression levels of both syncytins 1 and 2 fusogenic proteins and the degree of severity of preeclampsia symptoms PMID: 21493955
    29. HERV-W env triggers brain-derived neurotrophic factor (BDNF) production in human U251 glioma cells. PMID: 20100784
    30. Analysis of non-spliced ERVWE1 mRNAs and env mRNAs detected efficient splicing of endogenously expressed RNAs in trophoblastic but not in non-placental cells. PMID: 21771862
    31. CD9 increases GCM1 expression via the cAMP/PKA signaling pathway, resulting in the increase in ERVWE1 expression. PMID: 19692500
    32. HERV-W syncytin expression apparently does not play role in endometriosis. However, it may possibly influence development of endometriosis because of increased expression in normal endometrium in endometriosis patients. PMID: 19903052
    33. contributor to normal placental architecture, especially in the fusion processes of cytotrophoblasts to syncytiotrophoblasts. the gene expression of syncytin may be altered in cases with placental dysfunction such as preeclampsia or HELLP syndrome. PMID: 11854637
    34. mRNA abundance for syncytin showed stimulation by forskolin in BeWo cells PMID: 12175968
    35. syncytin-mediated trophoblastic fusion in human cells is regulated by GCMa PMID: 12397062
    36. Syncytin gene activation is highest in term placenta PMID: 12620933
    37. Hypoxia alters expression and function of syncytin and its receptor during trophoblast cell fusion of human placental BeWo cells: implications for impaired trophoblast syncytialisation in pre-eclampsia. PMID: 12757936
    38. Syncytin gene expression is down-regulated by hypoxia, which strengthens the hypothesis that syncytin is reduced in disturbed pregnancies in the course of placental hypoxia. PMID: 14520239
    39. syncytin promoter is located in the 5' long terminal repeat (LTR) of the HERV-W gene and that binding sites for CBF and Oct in the proximal promoter are critical for transcriptional regulation of the gene in trophoblast cells. PMID: 14613893
    40. relative L1 and HERV-W expression levels are elevated in representative samples of malignant vs. non-malignant ovarian tissues PMID: 15109395
    41. Cytoplasmic domain of syncytin is not essential for syncytin-mediated fusion but may play regulatory role. Intramolecular interaction between two heptad repeat regions (HRA and B) is involved in fusion process. PMID: 15269105
    42. Syncytin's proinflammatory properties in the nervous system demonstrate a novel role for an endogenous retrovirus protein, which may be a target for therapeutic intervention. PMID: 15452578
    43. The 146-bp region of the 5'-flanking region (nt-294/-148) of the human syncytin gene acts as a placenta-specific enhancer. PMID: 15888734
    44. GCMa-driven syncytin expression is the key mechanism for syncytiotrophoblast formation PMID: 16004993
    45. Syncytin transcripts were found in first-trimester trophoblast cells with both villous and extravillous phenotypes and also in the JAR and JEG-3 choriocarcinoma cell lines. PMID: 16760410
    46. evaluation of the interaction of the HERV-W envelope with the hASCT2 receptor; a region consisting of the N-terminal 124 amino acids of the mature glycoprotein surface subunit was determined as the minimal receptor-binding domain PMID: 16820059
    47. Syncytin is expressed by human cancer cells and is involved in cancer-endothelial cell fusions PMID: 16871371
    48. Median viral RNA levels for syncytin-1 were higher in brains of MS patients relative to non-MS patients and median syncytin-1 DNA levels in MS brains were higher than non-MS brain tissue. PMID: 17209768
    49. Transcripts from the HERV-W element on chromosome 7q21.2 encoding syncytin and from the SOD1 gene were detected at elevated levels in biopsies from the most affected muscles from MND patients compared to biopsies from control individuals. PMID: 17453631
    50. present evidence in support of the direct role for syncytin-A in mouse trophoblast stem cell fusion and differentiation involved in placental dev. PMID: 17762178

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  • 亚细胞定位：
    [Surface protein]: Cell membrane; Peripheral membrane protein.; [Transmembrane protein]: Cell membrane; Single-pass type I membrane protein.; [Syncytin-1]: Virion.
  • 蛋白家族：
    Gamma type-C retroviral envelope protein family, HERV class-I W env subfamily
  • 组织特异性：
    Expressed at higher level in placental syncytiotrophoblast. Expressed at intermediate level in testis. Seems also to be found at low level in adrenal tissue, bone marrow, breast, colon, kidney, ovary, prostate, skin, spleen, thymus, thyroid, brain and tra
  • 数据库链接：

    HGNC: 13525

    OMIM: 604659

    KEGG: hsa:30816

    STRING: 9606.ENSP00000419945