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  4. Recombinant Human T-cell leukemia virus 1 Protein Tax-1 (tax)

Recombinant Human T-cell leukemia virus 1 Protein Tax-1 (tax)

  • 货号：
    CSB-YP321200HQG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP321200HQG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP321200HQG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP321200HQG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP321200HQG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    tax
  • Uniprot No.：
  • 别名：
    tax; Protein Tax-1; Protein X-LOR; Protein PX; Trans-activating transcriptional regulatory protein of HTLV-1
  • 种属：
    Human T-cell leukemia virus 1 (isolate Caribbea HS-35 subtype A) (HTLV-1)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-353
  • 氨基酸序列
    MAHFPGFGQS LLFGYPVYVF GDCVQGDWCP ISGGLCSARL HRHALLATCP EHQITWDPID GRVIGSALQF LIPRLPSFPT QRTSKTLKVL TPPITHTTPN IPPSFLQAMR KYSPFRNGYM EPTLGQHLPT LSFPDPGLRP QNLYTLWGGS VVCMYLYQLS PPITWPLLPH VIFCHPGQLG AFLTNVPYKR IEKLLYKISL TTGALIILPE DCLPTTLFQP ARAPVTLTAW QNGLLPFHST LTTPGLIWTF TDGTPMISGP CPKDGQPSLV LQSSSFIFHK FQTKAYHPSF LLSHGLIQYS SFHNLHLLFE EYTNIPISLL FNEKEADDND HEPQISPGGL EPLSEKHFRE TEV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional activator that activates both the viral long terminal repeat (LTR) and cellular promoters via activation of CREB, NF-kappa-B, SRF and AP-1 pathways. Binds to three 21 bp repeat elements located within the LTRs, referred to as Tax-responsive elements (TRE). Binding to TRE requires the interaction with CREB1 and CREBBP. Also induces chromatin remodeling of proviral LTR-mediated gene expression by recruiting the histone acetyl transferases CREBBP and EP300 to the chromatin, which results in histone acetylation. Via its interaction with IKK regulatory subunit IKBKG, Tax-1 persistently stimulates I-kappa-B kinase (IKK), resulting in constitutive activation of the transcription factor NF-kappa-B. Induction of the nuclear expression of members of the NFkB family of transcription factors, which leads to up-regulated expression of many gene promoters containing NFkB motifs. These genes include those encoding IL2, IL15, IL2RA and IL15RA, leading to autocrine IL2/IL2RA and IL15/IL15RA loops. The resulting T-cell proliferation leads to malignant transformation and to the development of adult T-cell leukemia (ATL). IL13, known to be linked to leukemogenesis, is also up-regulated by Tax-1. Interaction with PDZ domain-containing proteins induce IL2-independent growth, which may be a factor in multi-step leukemogenesis. Inhibits the action of at least three cellular tumor suppressors p53/TP53, RB1 and DLG1, and suppresses their abilities to dictate apoptosis in primary cells. Required for viral replication.
  • 基因功能参考文献：
    1. Suggest that HBZ-mediated Foxp3 expression is partly associated with the pathogenesis of HTLV-I-seropositive Sjogren's syndrome. PMID: 29600938
    2. High tax expression is associated with persistent HTLV1 infection and leukemogenesis. PMID: 29358408
    3. By fluorescence resonance energy transfer (FRET), we detected the direct binding of Tax to DLG1 within the cell. The interaction specifically affects the cellular distribution of not only DLG1, but also Tax. The aggregates distribute into the Golgi apparatus in spatial association with the microtubule-organizing center (MTOC). PMID: 29168728
    4. HTLV-1 Tax protein interacts with the central helicase core domain of UPF1 and freezes it, which leads to nonsense-mediated mRNA decay inhibition, which in turn helps HTLV-1 to ensure its own genome expression. PMID: 29382845
    5. A Luciferase Functional Quantitative Assay for Measuring NF-kB Promoter Transactivation Mediated by HTLV-1 and HTLV-2 Tax Proteins. PMID: 28357663
    6. Human T-lymphotropic virus 1 Tax protein impairs K63-linked ubiquitination of STING and disrupted the interactions between STING and TBK1 to evade host innate immunity. PMID: 28119118
    7. Tax interacts with the host OGT/OGA complex and inhibits the activity of OGT-bound OGA. PMID: 28742148
    8. Human T-cell leukemia virus type 1 (HTLV-1) Tax directly binds to BCL11B. Tax enhances BCL11B degradation through proteasome pathway. Loss of BCL11B enhances cell growth in HTLV-1-infected cells. PMID: 28669733
    9. these results lead us to propose a novel model in which the hybrid-chain-dependent oligomerization of the IkappaB kinase (IKK) complex triggered by Tax leads to trans-autophosphorylation-mediated IKK activation. PMID: 28103322
    10. Tax effect on ERalpha induced activation of genes by the classical pathway by testing its influence on E2-induced expression of Eestrogen response element promoter-driven luciferase reporter , is reported. PMID: 27420286
    11. In HIV-infected primary resting CD4(+) T cells, Tax-1 reactivated HIV-1 transcription up to five fold in an ex vivo latency model, these results confirm that HTLV-1/Tax hijacks cellular partners, promoting HIV-1 transcription. PMID: 28152383
    12. Tax and semaphorin 4D released from lymphocytes infected with human lymphotropic virus type 1 and inhibit neurite growth in a neuron cell line. PMID: 26389656
    13. evaluated Tax secretion in peripheral blood mononuclear cell cultures from human T-lymphotropic virus-type 1-associated myelopathy/tropical spastic paraparesis patients (HAM/TSP) and asymptomatic carriers; also, Tax plasma level was evaluated from other HAM/TSP patients and asymptomatic carriers PMID: 26241614
    14. EGR-1 overexpression in Jurkat cells transfected to express Tax, promoted the activation of several genes, especially those that contained AP-1 (Jun/c-Fos), but knockdown of endogenous EGR-1 by siRNA reduced Tax-mediated JNK-1 transcription PMID: 26573109
    15. Our studies revealed the biochemical function of Tax in the process of IKK activation PMID: 27082114
    16. Tax1-mediated cell survival and death depend on the cell growth phase. PMID: 26829041
    17. Thus, CIITA inhibits cytoplasmic and nuclear steps of human T cell lymphotropic virus type 1 Tax-1-mediated NF-kappaB activation. PMID: 26792751
    18. HIC specifically interacts with HTLV-1 Tax and negatively regulates Tax transactivational activity by altering its subcellular distribution and stability. PMID: 26469549
    19. Study used molecular combing techniques to study the effect of the oncoprotein Tax on DNA replication and found that replication forks have difficulties replicating complex DNA, fork progression is slower, and they pause or stall more frequently in the presence of Tax expression. PMID: 25185513
    20. unveiled a previously unidentified role of Foxp3 in T cell transformation, providing a molecular basis for HTLV-1 tax protein transformation of CD4+Foxp3+ T cells PMID: 26381169
    21. Study shows selective phosphorylation of PKCdelta in HTLV1infected T cells where Tax activates NFkappaB through PKCdelta activation. PMID: 25625567
    22. These results suggest that HTLV-I induces CD83 expression on T cells via Tax1 -mediated NF-kappaB activation, which may promote HTLV-I infection in vivo. PMID: 26129803
    23. Mechanistically, MEF-2 was recruited to the viral promoter (LTR, long terminal repeat) in the context of chromatin, and constituted Tax/CREB transcriptional complex via direct binding to the HTLV-1 LTR. PMID: 25809782
    24. Tax interacted with and activated TRAF6, and triggered its mitochondrial localization, where it conjugated four carboxyl-terminal lysine residues of MCL-1 with lysine 63-linked polyubiquitin chains PMID: 25340740
    25. Tax-1 but not Tax-2 induces cell transformation and increased plasmatocyte proliferation. PMID: 25995252
    26. The Tax oncogene enhances ELL incorporation into p300 and P-TEFb containing protein complexes to activate transcription. PMID: 26188510
    27. Results indicate that Tax-induced downregulation of Bim in HTLV-1-infected T cells promotes their IL-2-independent growth, thereby supporting the persistence of HTLV-1 infection in vivo. PMID: 25175936
    28. Tax confers apoptosis resistance to HTLV-1-infected T cells by suppressing the expression of Bim and Bid. PMID: 25522269
    29. Tax is responsible for suppressing BCL11B protein expression in HTLV-1-infected T-cells; Tax-mediated repression of BCL11B is another mechanism that Tax uses to promote oncogenesis of HTLV-1-infected T-cells. PMID: 25613934
    30. Tax potently inhibits the activity of DEx(D/H) box helicases RIG-I and MDA5 as well as Toll-dependent TIR-domain-containing adapter-inducing interferon-beta (TRIF), which function as cellular sensors or mediators of viral RNA. PMID: 25694597
    31. study identified TCF1 and LEF1 as Tax antagonistic factors in vivo, a fact which may critically influence the peripheral T-cell tropism of this virus PMID: 25646419
    32. Data suggest that Tax recruitment of autophagic molecules to lipid rafts is a dominant strategy to deregulate autophagy in the context of HTLV-1 transformation of T cells. PMID: 24362528
    33. HTLV-1 Tax is recruited into the TBK1/IKKvarepsilon complexes as a scaffolding-adaptor protein that enhances IFN-beta gene expression. PMID: 25531185
    34. exosomes released from HTLV-1-infected Tax-expressing cells contributed to enhanced survival of exosome-recipient cells when treated with Fas antibody PMID: 24939845
    35. HTLV-1 Tax SUMOylation is not required for NF-kappaB-driven gene induction. PMID: 24991007
    36. Tax transactivates the ELL2 promoter in HTLV-1-infected T-cells. PMID: 25058508
    37. The interaction between human T cell lymphotropic virus type-I Tax and calreticulin was determined by confocal microscopy and coimmunoprecipitation. PMID: 24321043
    38. NF-kappaB-binding cofactor inhibitor of NF-kappaB-zeta (IkappaB-zeta) is constitutively expressed in HTLV-I-infected T cell lines and ATL cells, and Tax transactivates the IkappaB-zeta gene, mainly through NF-kappaB. PMID: 24027435
    39. Brd4 as a key regulator for Tax-mediated NF-kappaB gene expression PMID: 24189064
    40. Inhibition of HSP90 activity in HTLV-1-transformed cells elicited proteasomal degradation of Tax in the nuclear matrix and inhibition of NF-kappaB and HTLV-1 long terminal repeat activation. PMID: 24109220
    41. These data suggest that the expression of Tax among the different target cells may explain the variable presentation of HTLV-1 infections. PMID: 23816512
    42. These findings indicate that Tax enhances the expression of high mobility group box 1 gene at the transcriptional level, possibly by interacting with C/EBP. PMID: 23534754
    43. USP10 is a host factor that inhibits stress-induced ROS production and apoptosis in HTLV-1-infected T cells; however, its activities are attenuated by Tax. PMID: 23775713
    44. Our results suggest that HTLV-1 Tax1 fails to activate the canonical pathway of NF-kappaB in non-hematopoietic cell lines. PMID: 23791017
    45. s identified acetyltransferase p300 and deacetylase HDAC7 as enzymes modulating human T cell leukemia virus type 1 Tax protein acetylation. PMID: 23880157
    46. Group I p21-activated kinases augment Tax-mediated transcriptional activation of human T-cell leukemia virus type 1 long terminal repeats in a kinase-independent manner. PMID: 23622267
    47. Tax-specific cytotoxic T-lymphocyte cell treatment significantly decreases human soluble IL-2Rgamma serum concentrations and prolongation of survival time in a mouse model of human adult T cell leukemia/lymphoma. PMID: 23733874
    48. These findings revealed a new function of LKB1 and salt-inducible kinases as negative regulators of HTLV-1 transcription. PMID: 23577667
    49. Nevertheless, both human T cell leukemia virus type 2 and human T cell leukemia virus type 2 Tax preferentially immortalized CD4(+) T cells, but not CD8(+) T cells. PMID: 23054433
    50. Both Tax-1 and Tax-2 activate the NF-kappaB pathway via similar mechanisms involving ubiquitination and sumoylation. PMID: 23217160

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  • 亚细胞定位：
    Host nucleus. Host cytoplasm.
  • 蛋白家族：
    Deltaretrovirus Tax protein family
  • 数据库链接：

    KEGG: vg:1491938