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  4. Recombinant Human TIMELESS-interacting protein (TIPIN)

Recombinant Human TIMELESS-interacting protein (TIPIN)

  • 中文名称：
    人TIPIN重组蛋白
  • 货号：
    CSB-YP880115HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人TIPIN重组蛋白
  • 货号：
    CSB-EP880115HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人TIPIN重组蛋白
  • 货号：
    CSB-EP880115HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人TIPIN重组蛋白
  • 货号：
    CSB-BP880115HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人TIPIN重组蛋白
  • 货号：
    CSB-MP880115HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    TIPIN
  • Uniprot No.：
  • 别名：
    FLJ20516; TIMELESS interacting protein; TIMELESS-interacting protein; TIPIN; TIPIN_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-301
  • 氨基酸序列
    MLEPQENGVI DLPDYEHVED ETFPPFPPPA SPERQDGEGT EPDEESGNGA PVRVPPKRTV KRNIPKLDAQ RLISERGLPA LRHVFDKAKF KGKGHEAEDL KMLIRHMEHW AHRLFPKLQF EDFIDRVEYL GSKKEVQTCL KRIRLDLPIL HEDFVSNNDE VAENNEHDVT STELDPFLTN LSESEMFASE LSRSLTEEQQ QRIERNKQLA LERRQAKLLS NSQTLGNDML MNTPRAHTVE EVNTDEDQKE ESNGLNEDIL DNPCNDAIAN TLNEEETLLD QSFKNVQQQL DATSRNITEA R
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays an important role in the control of DNA replication and the maintenance of replication fork stability. Important for cell survival after DNA damage or replication stress. May be specifically required for the ATR-CHEK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light. Forms a complex with TIMELESS and this complex regulates DNA replication processes under both normal and stress conditions, stabilizes replication forks and influences both CHEK1 phosphorylation and the intra-S phase checkpoint in response to genotoxic stress.
  • 基因功能参考文献：
    1. the 1.85 A crystal structure of a large N-terminal segment of human Timeless, spanning amino acids 1-463, is presented and this region of human Timeless harbours a partial binding site for Tipin. PMID: 28334766
    2. TIPIN is important for the maintenance of DNA replication and represents a potential treatment target for the worst prognosis associated breast cancers, such as Triple-negative breast cancer. PMID: 26004086
    3. Tim-Tipin complex (or Tim alone) is able to associate with DNA polymerase epsilon bound to a 40-/80-mer DNA ligand. PMID: 23511638
    4. Tim-Tipin complex might play a role in coupling DNA unwinding and DNA synthesis by directly affecting the catalytic activities of replication fork proteins. PMID: 23359676
    5. We now show that cellular DNA replication fork pausing and protection factors Timeless (Tim) and Tipin (Timeless-interacting protein) accumulate at OriP during S phase of the cell cycle. PMID: 21490103
    6. RPA-covered ssDNA not only supports recruitment and activation of ATR but also, through Tipin and Claspin, it plays an important role in the action of ATR on its critical downstream target Chk1 PMID: 20233725
    7. The results suggest that Timeless-Tipin functions as a replication fork stabilizer that couples DNA replication with sister chromatid cohesion established at replication forks. PMID: 20124417
    8. Tipin is a checkpoint mediator that cooperates with Tim and may regulate the nuclear relocation of Claspin in response to replication checkpoint PMID: 17102137
    9. observation explains the similar checkpoint phenotypes observed in both Tipin- and Timeless-depleted cells PMID: 17116885
    10. TIM and Tipin are functional orthologs of their replisome-associated yeast counterparts capable of coordinating replication with genotoxic stress responses, and distinguishes mammalian TIM from the circadian-specific paralogs. PMID: 17141802
    11. These findings indicate that the Tim-Tipin complex mediates the UV-induced intra-S checkpoint, Tim is needed to maintain DNA replication fork movement, Tipin interacts with RPA on DNA. PMID: 17296725

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    CSM3 family
  • 数据库链接：

    HGNC: 30750

    OMIM: 610716

    KEGG: hsa:54962

    STRING: 9606.ENSP00000261881

    UniGene: Hs.744308