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  4. Recombinant Human Telomerase Cajal body protein 1 (WRAP53)

Recombinant Human Telomerase Cajal body protein 1 (WRAP53)

  • 中文名称：
    人WRAP53重组蛋白
  • 货号：
    CSB-YP883605HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人WRAP53重组蛋白
  • 货号：
    CSB-EP883605HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人WRAP53重组蛋白
  • 货号：
    CSB-EP883605HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人WRAP53重组蛋白
  • 货号：
    CSB-BP883605HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人WRAP53重组蛋白
  • 货号：
    CSB-MP883605HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    WRAP53
  • Uniprot No.：
  • 别名：
    DKCB3; FLJ10385; TCAB1; Telomerase Cajal body protein 1; WAP53_HUMAN; WD repeat containing antisense to TP53; WD repeat containing protein 79; WD repeat-containing protein 79; WD40 repeat-containing protein encoding RNA antisense to p53; Wrap53
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-548
  • 氨基酸序列
    MKTLETQPLA PDCCPSDQDP APAHPSPHAS PMNKNADSEL MPPPPERGDP PRLSPDPVAG SAVSQELREG DPVSLSTPLE TEFGSPSELS PRIEEQELSE NTSLPAEEAN GSLSEEEANG PELGSGKAME DTSGEPAAED EGDTAWNYSF SQLPRFLSGS WSEFSTQPEN FLKGCKWAPD GSCILTNSAD NILRIYNLPP ELYHEGEQVE YAEMVPVLRM VEGDTIYDYC WYSLMSSAQP DTSYVASSSR ENPIHIWDAF TGELRASFRA YNHLDELTAA HSLCFSPDGS QLFCGFNRTV RVFSTARPGR DCEVRATFAK KQGQSGIISC IAFSPAQPLY ACGSYGRSLG LYAWDDGSPL ALLGGHQGGI THLCFHPDGN RFFSGARKDA ELLCWDLRQS GYPLWSLGRE VTTNQRIYFD LDPTGQFLVS GSTSGAVSVW DTDGPGNDGK PEPVLSFLPQ KDCTNGVSLH PSLPLLATAS GQRVFPEPTE SGDEGEELGL PLLSTRHVHL ECRLQLWWCG GAPDSSIPDD HQGEKGQGGT EGGVGELI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    RNA chaperone that plays a key role in telomere maintenance and RNA localization to Cajal bodies. Specifically recognizes and binds the Cajal body box (CAB box) present in both small Cajal body RNAs (scaRNAs) and telomerase RNA template component (TERC). Essential component of the telomerase holoenzyme complex, a ribonucleoprotein complex essential for the replication of chromosome termini that elongates telomeres in most eukaryotes. In the telomerase holoenzyme complex, required to stimulate the catalytic activity of the complex. Acts by specifically binding the CAB box of the TERC RNA and controlling the folding of the CR4/CR5 region of the TERC RNA, a critical step for telomerase activity. In addition, also controls telomerase holoenzyme complex localization to Cajal body. During S phase, required for delivery of TERC to telomeres during S phase and for telomerase activity. In addition to its role in telomere maintenance, also required for Cajal body formation, probably by mediating localization of scaRNAs to Cajal bodies. Also plays a role in DNA repair: phosphorylated by ATM in response to DNA damage and relocalizes to sites of DNA double-strand breaks to promote the repair of DNA double-strand breaks. Acts by recruiting the ubiquitin ligase RNF8 to DNA breaks and promote both homologous recombination (HR) and non-homologous end joining (NHEJ).
  • 基因功能参考文献：
    1. a unique homozygous WRAP53 mutation site underlies the development of dyskeratosis congenita in a Chinese Han family PMID: 29514627
    2. Wrap53 is likely a potential oncogene or possesses oncogenic activity in colorectal cancer, promoting colorectal tumorigenesis. PMID: 30175821
    3. The results collectively suggest that WDR79 and SMN play evolutionarily conserved cooperative functions in the nervous system and suggest that WDR79/TCAB1 may have the potential to modify SMA pathogenesis. PMID: 28502804
    4. in response to DNA damage the Cajal body protein WRAP53beta is phosphorylated by ATM and that this phosphorylation is important for the recruitment of WRAP53beta to DNA lesions, its interaction with gammaH2AX, subsequent localization of the downstream repair factor 53BP1 to DNA breaks, as well as for HR and NHEJ repair. PMID: 27715493
    5. The present findings indicate that WRAP53beta and RNF8 are rate-limiting factors in the repair of DNA double-strand breaks and raise the possibility that upregulation of WRAP53beta may contribute to genomic stability in and survival of cancer cells. PMID: 27310875
    6. WDR79 colocalized and interacted with USP7 in the nucleus of non-small cell lung cancer cells. This event, in turn, reduced the ubiquitination of Mdm2 and p53, thereby increasing the stability and extending the half-life of the two proteins. PMID: 28406480
    7. The depletion of WRAP53 inhibits the proliferation of lung-adenocarcinoma A549 and SPC-A-1 cells via G1/S cell-cycle arrest. Several proteins interacting with WRAP53 were identified through co-immunoprecipitation and liquid chromatography/mass spectrometry. PMID: 28347242
    8. we analyzed the association between the WRAP53 gene rs2287499 C>G polymorphism and risk of cancer using five case-control studies.In the overall analysis, no significant association between rs2287499 and risk of cancer was found. PMID: 27525856
    9. The interaction of MDC1 with RNF8, but not with ATM requires WRAP53beta, suggesting that WRAP53beta facilitates the former interaction without altering phosphorylation of MDC1 by ATM. PMID: 26734725
    10. Moreover, our current observations identify the nuclear levels of WRAP53beta as a promising biomarker for the survival of patients with ovarian cancer. PMID: 26426684
    11. the sub-cellular localization of the WRAP53 protein has a significant impact on breast cancer survival. PMID: 26460974
    12. This study showed that UCA1 and WRAP53 upregulation may serve as novel serum biomarkers for hepatocellular carcinoma diagnosis and prognosis. PMID: 26551349
    13. WRAP53 is associated with development and progression of esophageal squamous cell carcinoma PMID: 24626331
    14. Decreasing the expression of WRAP53 using RNA interference technique can enhance the radiosensitivity. PMID: 25854392
    15. The telomerase holoenzyme Cajal body-associated protein, TCAB1, was released from telomerase RNA in mitotic cells coincident with TCAB1 delocalization from Cajal bodies. PMID: 26170453
    16. nuclear expression of WRAP53beta promotes tumor cell death in response to radiotherapy and is a promising predictor of radiotherapy response in patients with HNSCC PMID: 25456005
    17. The data indicated that TCAB1 might facilitate the occurrence and development of head and neck carcinomas. PMID: 25070141
    18. Studied the association of a frequent genetic variation in WRAP53, rs2287499 (C/G), with breast cancer risk and prognosis among Iranian-Azeri population. PMID: 25134915
    19. Study finds that TRiC is required for folding the telomerase cofactor TCAB1, which controls trafficking of telomerase and small Cajal body RNAs (scaRNAs). PMID: 25467444
    20. WRAP53beta as a novel regulator of DSB repair by providing a scaffold for DNA repair factors. PMID: 25512560
    21. SNPs in WRAP53 (rs2287497 and rs2287498) have stronger association with an ovarian cancer risk than rs1042522 in TP53. PMID: 23192612
    22. Intronic mutation outside overlapping regions of p53/wrap53 genes are associated with breast cancer. PMID: 23886136
    23. Examined expression of WRAP53 protein in rectal cancers and analyzed its relationship to the response to preoperative radiotherapy and patient survival.In radiotherapy group, positive WRAP53 in the metastasis correlated with better survival. PMID: 22805008
    24. although Cajal bodies are important for recruitment of telomerase, TCAB1 has an additional role in this process that is independent of these structures PMID: 22547674
    25. Knockdown of the WRAP53 protein triggers massive apoptosis through the mitochondrial pathway. PMID: 21368886
    26. WRAP53 mediates the interaction between SMN and associated proteins, which is important for nuclear targeting of SMN and the subsequent localization of the SMN complex to Cajal bodies PMID: 21072240
    27. Compound heterozygous mutations in TCAB1 disrupt telomerase localization to Cajal bodies, resulting in misdirection of telomerase RNA to nucleoli, which prevents telomerase from elongating telomeres PMID: 21205863
    28. Studies show that a linked, positively selected allele at the nearby gene WDR79 may be partly responsible for the sequence diversity profile of TP53. PMID: 19797907
    29. Common variation in TP53 or WDR79 could be associated with ER negative breast cancers. PMID: 17683073
    30. identification of telomerase holoenzyme subunit, TCAB1, enriched in Cajal bodies; it associates with active telomerase, telomerase components & small Cajal body RNAs; TCAB1 controls telomerase trafficking & required for telomere synthesis in cancer cells PMID: 19179534
    31. We have identified a natural antisense transcript of p53, designated Wrap53, that regulates endogenous p53 mRNA levels and further induction of p53 protein by targeting the 5' untranslated region of p53 mRNA. PMID: 19250907

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  • 相关疾病：
    Dyskeratosis congenita, autosomal recessive, 3 (DKCB3)
  • 亚细胞定位：
    Nucleus, Cajal body. Chromosome, telomere. Chromosome.
  • 组织特异性：
    Expressed in all tissues and cell lines examined.
  • 数据库链接：

    HGNC: 25522

    OMIM: 612661

    KEGG: hsa:55135

    STRING: 9606.ENSP00000324203

    UniGene: Hs.408312