Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Human Telomeric repeat-binding factor 1 (TERF1)

Recombinant Human Telomeric repeat-binding factor 1 (TERF1)

  • 货号：
    CSB-YP023386HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP023386HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP023386HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP023386HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP023386HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    hTRF1 AS; NIMA interacting protein 2; NIMA-interacting protein 2; PIN 2; PIN2; t TRF1; Telomeric protein Pin2; Telomeric protein Pin2/TRF1; Telomeric repeat binding factor (NIMA interacting) 1; Telomeric repeat binding factor 1; Telomeric repeat binding protein 1; Telomeric repeat-binding factor 1; TERF 1; Terf1; TERF1_HUMAN; TRBF 1; TRBF1; TRF 1; TRF; TTAGGG repeat binding factor 1; TTAGGG repeat-binding factor 1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-439
  • 氨基酸序列
    AEDVSSAAP SPRGCADGRD ADPTEEQMAE TERNDEEQFE CQELLECQVQ VGAPEEEEEE EEDAGLVAEA EAVAAGWMLD FLCLSLCRAF RDGRSEDFRR TRNSAEAIIH GLSSLTACQL RTIYICQFLT RIAAGKTLDA QFENDERITP LESALMIWGS IEKEHDKLHE EIQNLIKIQA IAVCMENGNF KEAEEVFERI FGDPNSHMPF KSKLLMIISQ KDTFHSFFQH FSYNHMMEKI KSYVNYVLSE KSSTFLMKAA AKVVESKRTR TITSQDKPSG NDVEMETEAN LDTRKSVSDK QSAVTESSEG TVSLLRSHKN LFLSKLQHGT QQQDLNKKER RVGTPQSTKK KKESRRATES RIPVSKSQPV TPEKHRARKR QAWLWEEDKN LRSGVRKYGE GNWSKILLHY KFNNRTSVML KDRWRTMKKL KLISSDSED
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.
  • 基因功能参考文献：
    1. Findings suggest that rescuing naturally decreased TRF1 levels during mouse aging using AAV9-TRF1 gene therapy results in an improved health span. PMID: 28944611
    2. Results provide evidence that TRF1 phosphorylation on T271 plays an important role in modulating its activity in both telomerease-expressing and alternative lengthening of telomeres cells. PMID: 27841304
    3. Both TRF1 and TRF2 were overexpressed in prostate cancer. There was no specificity of TRF2 in prostate cancer, while TRF1 may be associated with prostate cancer progression. PMID: 28808664
    4. study suggests that, gallstone does not affect telomere length and even after having increased telomere length, decreased expression of some shelterin genes in inflamed tissue might cause telomeres to cap improperly, possibly leading to telomere dysfunction and further, gallbladder carcinogenesis PMID: 28643740
    5. Data show that TRF1 expression is increased in both human and mouse glioblastoma multiforme (GBM). PMID: 29136505
    6. our results suggest that RhoGDIalpha regulate TRF1 and telomere length and may be novel prognostic biomarkers in colorectal cancer PMID: 28417530
    7. TRF1 phosphorylation by Nek7 favors the binding of shelterin protein TIN2 and disfavors E3 ligase Fbx4 interaction, thus preventing TRF1 ubiquitination and proteasome degradation to maintain telomere integrity. PMID: 28216227
    8. Cdk-dependent phosphorylation of TRF1 on threonine 371 promotes TRF1 to interact with APBs in S and G2 phases independently of its binding to telomeric DNA. We have demonstrated that the interaction of (pT371)TRF1 with APBs is dependent upon ATM and homologous-recombination-promoting factors such as Mre11 and BRCA1. PMID: 27185864
    9. TRF1 tethers SA1 within telomeric regions that SA1 transiently interacts with. SA1 and TRF1 together form longer DNA-DNA pairing tracts than with TRF1 alone, as revealed by atomic force microscopy imaging PMID: 27298259
    10. data suggest that NUB1 participates in telomere maintenance by regulating the levels of TRF1 at telomeres through both NEDD8-dependent and NEDD8-independent pathways. PMID: 27214791
    11. Data indicate that overexpression of telomeric repeat binding factor 1 (TRF1) in aging endothelial cells (EC) reduced telomere-associated DNA damage foci. PMID: 26658719
    12. we provide mechanistic insight into the role of TRF1 in liver regeneration and provide a mouse model recapitulating the clinical features of LLCC. PMID: 25819337
    13. TRF1 links TopoIIalpha and SAC proteins in a pathway that ensures correct telomere replication and mitotic segregation, unveiling how TRF1 protects from telomere fragility and mitotic defects. PMID: 24626180
    14. ZSCAN4 indirectly interacts with TRF1 in cancer cells. We show that ZSCAN4 plays an important role independent of telomere maintenance pathways (telomerase positive and alternative pathway) or cell lineage. PMID: 26403970
    15. analysis of human telomere repeat binding factor 1 (hTRF1) in complex with Escherichia coli Hsp70 (DnaK) PMID: 26240333
    16. Results showed significant interaction between variants at TERF1 and AFAP1L2 loci. Given the key role of TERF1 in telomere biology and its physical interaction with AFAP1L2, these results support a role for telomere dysfunction in melanoma development. PMID: 25892537
    17. Modulating miR-155 expression in cells altered TRF1 levels and TRF1 abundance at telomeres. Compromising TRF1 expression by elevating miR-155 increased telomere fragility and altered the structure of metaphase chromosomes. PMID: 24876105
    18. TAP68 functions in mediating TRF1-tankyrase 1 localization to the centrosome and in mitotic regulation PMID: 24692559
    19. this study demonstrates an essential role of TRF1 in the centromeric localization of Aurora-B kinase, which is required for correction of the merotelic attachment of microtubules to a single kinetochore and for proper chromosome segregation. PMID: 24752893
    20. TRF1 knockdown suppressed Nek2- induced lagging chromosomes, suggesting that TRF1 may affect kinetochore capture by mitotic spindles. PMID: 24091727
    21. these findings suggested that PinX1 may maintain telomere integrity by regulating TRF1 stability and that hTERT may act as both a positive and a negative regulator of TRF1 homeostasis in a PinX1-dependent manner. PMID: 24415760
    22. U2AF65 represents a new route for modulating TRF1 function at telomeres. PMID: 24389012
    23. Data show that RTEL1 interacts with the shelterin protein TRF1, indicating a potential recruitment mechanism of RTEL1 to telomeres. PMID: 23959892
    24. TRF1 is an important factor in the colorectal tumor progression and maybe a diagnostic factor PMID: 23268632
    25. This improved model also reveals extensive indirect readout, which suggests that telomere recognition by TRF1 will be affected by changes to DNA helical parameters such as bending. PMID: 23702294
    26. Results indicate that beta-TrCP1 is involved in the negative regulation of TRF1. PMID: 23583392
    27. Low TRF1 expression is associated with chronic lymphocytic leukemia. PMID: 22878603
    28. TRF1 binds to and stabilizes common fragile site on Chromosome 2q14. PMID: 22790221
    29. The TRF1 stimulates the binding and enzymatic activities of the LP-BER proteins APE1, FEN1 and LigI both individually and when they act together in reconstituted LP-BER using a telomeric substrate. PMID: 22336916
    30. The nucleostemin(NS)could promote the recruitment of PML-IV to SUMOylated TRF1 in TA(+) and ALT cells. PMID: 22641345
    31. Age-dependent telomere shortening occurs in Duchenne muscular dystrophy muscles and is associated with overexpression of mRNA and protein levels of TRF1 and poly (ADP-ribose) polymerase (PARP)1. PMID: 20137830
    32. one function of PinX1 is to stabilize TRF1 during mitosis, perhaps to promote transition into M phase of the cell cycle. PMID: 22331467
    33. It is found that water molecules around the DNA are more structured with significantly higher density and ordering than that around the protein in the complex. PMID: 22225189
    34. NS inhibits TRF1 dimerization and shortens TRF1 association with the telomere. PMID: 22045740
    35. A subset of TRF1 and TRF2 binding sites correspond to nontelomeric satellite DNA repeats. PMID: 21423270
    36. Mouse gene deletion experiments revealed DNA-damage-response pathways that threaten chromosome ends and how the components of the telomeric shelterin complex prevent activation of these pathways.[Shelterin] PMID: 21209389
    37. the telomerase inhibitor PinX1 is recruited to telomeres by TRF1 and provides a critical link between TRF1 and telomerase inhibition to prevent telomere elongation and help maintain telomere homeostasis. PMID: 21119197
    38. Monoclonal gammopathy of undetermined significance patients showed increased TRF1 levels and lower expression of TRF2 and TANK1 compared with multiple myeloma patients. PMID: 20644899
    39. the protein network surrounding telomere repeat binding factors, TRF1, TRF2, and POT1 using dual-tag affinity purification PMID: 20811636
    40. The expression of TRF1 may work to reduce the telomere length in precancerous lesions and offer new insight into the mechanism of carcinogenesis in gastric cancer. PMID: 20127252
    41. Atomic Force Microscopy imaging shows that hTRF1 induces compaction of telomeric DNA only in the presence of a nucleosome, suggesting that this compaction occurs through hTRF1-nucleosome interactions. PMID: 20056655
    42. results reveal an atypical small GTPase domain within Fbx4 as a substrate-binding motif for SCF(Fbx4) and uncover a mechanism for selective ubiquitination and degradation of TRF1 in telomere homeostasis control PMID: 20159592
    43. Kinetic analysis of DNA-dependent intrinsic tryptophan fluorescence quenching of hTRF1 revealed a two step binding process that is impacted by telomere repeat length, position, and structure PMID: 19887064
    44. nuclear localization signal and nuclear export signal sequences in NSCLCs patients did not have mutations. PMID: 19746267
    45. inhibition of Pin2/TRF1 in A-T cells is able to bypass the requirement for ATM in specifically restoring telomere shortening, the G(2)/M checkpoint defect, and radiosensitivity PMID: 11744712
    46. Isoform PIN2 interacts with the human SALL1 gene product. PMID: 11751684
    47. tumour growth does not seem to depend on cell proliferation but on TRF1 immunoexpression PMID: 11813863
    48. The telomeric poly(ADP-ribose) polymerase, tankyrase 1, contains multiple binding sites for telomeric repeat binding factor 1 (TRF1) and a novel acceptor, 182-kDa tankyrase-binding protein (TAB182). PMID: 11854288
    49. Results showed that targeting of TRF1 and TRF2 to specific telomeres could be induced, and that targeting leads to telomere shortening. This indicates that these proteins act in cis to repress telomere elongation. PMID: 11971978
    50. Gastric carcinomas with high TRF1 expression may require a large quantity of hRap1/ PMID: 12007281

    显示更多

    收起更多

  • 亚细胞定位：
    Nucleus. Cytoplasm, cytoskeleton, spindle. Chromosome, telomere. Note=Colocalizes with telomeric DNA in interphase and prophase cells. Telomeric localization decreases in metaphase, anaphase and telophase. Associates with the mitotic spindle.
  • 组织特异性：
    Highly expressed and ubiquitous. Isoform Pin2 predominates.
  • 数据库链接：

    HGNC: 11728

    OMIM: 600951

    KEGG: hsa:7013

    STRING: 9606.ENSP00000276603

    UniGene: Hs.442707