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  4. Recombinant Human Thyroxine 5-deiodinase (DIO3), partial

Recombinant Human Thyroxine 5-deiodinase (DIO3), partial

  • 货号：
    CSB-YP345038HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP345038HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP345038HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP345038HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP345038HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    DIO3
  • Uniprot No.：
  • 别名：
    5DIII; D3; Deiodinase iodothyronine type 3; Deiodinase iodothyronine type III; Dio 3; Dio3; DIOIII; IOD3_HUMAN; Iodothyronine deiodinase placental type; MGC124117; MGC124118; Thyroxine deiodinase type III ; Thyroxine deiodinase type III (selenoprotein); TXDI3; Type 3 DI; Type 3 iodothyronine selenodeiodinase; Type III 5' deiodinase; Type III iodothyronine deiodinase; Type-III 5''-deiodinase
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into RT3 (3,3',5'-triiodothyronine) and of T3 (3,5,3'-triiodothyronine) into T2 (3,3'-diiodothyronine). RT3 and T2 are inactive metabolites. May play a role in preventing premature exposure of developing fetal tissues to adult levels of thyroid hormones. Can regulate circulating fetal thyroid hormone concentrations throughout gestation. Essential role for regulation of thyroid hormone inactivation during embryological development.
  • 基因功能参考文献：
    1. The expression of DIO3 on mRNA/protein levels in the depressive disorders patients was increased in comparison to the control subjects. PMID: 29182613
    2. The expression of piRNAs encoded at DLK1-DIO3 enhances the prognostic potential of small non-coding RNAs specific to this locus in predicting lung cancer patient outcome. PMID: 27829231
    3. A critical role for reduced intracellular TH concentrations in neutrophil function during infection, for which the TH inactivating enzyme D3 appears essential. PMID: 29186449
    4. In neonatal skin, DIO3 exhibited a high degree of monoallelic expression from the paternal allele. PMID: 27329732
    5. Presence and subcellular location of D3 in human neutrophils for the first time and propose a model, in which D3 plays a role in the bacterial killing capacity of neutrophils either through generation of iodide for the myeloperoxidase system or through modulation of intracellular Thyroid hormone bioavailability. PMID: 27355490
    6. MAPK and SHH pathways modulate type 3 deiodinase expression in papillary thyroid carcinoma PMID: 26825960
    7. D3 is a critical factor in testis development and in testicular protection from thyrotoxicosis. PMID: 26727108
    8. the microRNA cluster at the Dlk1-Dio3 locus is upregulated in lung adenocarcinoma PMID: 24317514
    9. [review] D3 polymorphisms show no relationship with inter-individual variation in serum thyroid hormone parameters. PMID: 24878678
    10. Overall, the data suggest that active expression of the DLK1-DIO3 cluster represents a new biomarker for epigenetic stability of hESCs and indicates the importance of using a proper physiological oxygen level during the derivation and culture of hESCs. PMID: 24123616
    11. the imprinted DLK1-DIO3 locus is regulated by noncoding RNA IPW in an induced pluripotent stem cell model of Prader-Willi syndrome PMID: 24816254
    12. Data indicate that Na+/I- symporter and type 3 iodothyronine deiodinase genes are expressed in term placenta and amniotic membrane. PMID: 23857380
    13. Treatment with high affinity amyloidbeta42 oligomer-binding enantiomeric D3 peptide significantly decreases amyloidbeta deposits and the associated inflammatory response and improves cognition, even when applied only at late stages and high age. PMID: 23271316
    14. D3 expression is re-activated in various types of human cancers. It may play a role in carcinogenesis and neoplastic cell proliferation. Review. PMID: 23235188
    15. Reviewed are the microRNAs within the DLK1-DIO3 genomic region, their role in controlling tissue homeostasis and in the pathogenesis of some human diseases, mostly cancer, when aberrantly expressed. [review] PMID: 22825660
    16. Deiodinase iodothyronine type III activity was increased in all papillary thyroid carcinomas as compared with that in adjacent thyroid tissue, parallel with ~ 5 times increase in deiodinase iodothyronine type III mRNA levels. PMID: 22823995
    17. D3 expression in perinatal pancreatic beta-cells prevents untimely exposure to thyroid hormone, the absence of which leads to impaired beta-cell function and subsequently insulin secretion and glucose homeostasis PMID: 21828183
    18. coordinate up-regulation of the DLK1-DIO3 miRNA cluster at 14q32.2 may define a novel molecular (stem cell-like) subtype of hepatocellular carcinoma associated with poor prognosis PMID: 21737452
    19. [review] progress made in understanding the physiological function and significance of D3; summarizes the intriguing evidence that D3 plays a pivotal role in defining local TH concentration in the developing fetus and in several conditions in adult life. PMID: 21398344
    20. The minor allele of the DIO3 variant rs945006 showed suggestive evidence for protective association in the overall meta-analyses, which was supported by individual osteoarthritis studies and osteoarthritis subtypes. PMID: 20724312
    21. In this case-control study, homozygosity for type 2 deiodinase Thr92Ala polymorphism is associated with increased risk for type 2 diabetes. PMID: 20566590
    22. The expression of D3 mRNA was more than 10 times higher in the tumor tissue than the mean of placental tissues PMID: 19548001
    23. study demonstrates that D3 is located in the plasma membrane with most of its molecule in the extracellular space, allowing access to inactivate both the hormone T3 and prohormone T4 PMID: 12419801
    24. the SeC residue in the catalytic center of D3 is essential for efficient inner-ring deiodination of T(3) and in particular T(4) at physiological substrate concentrations. PMID: 12746313
    25. D3 contains a glycoside hydrolase clan GH-A-like structure PMID: 12847093
    26. identified a conserved enhancer located 3' of the DIO3 gene containing putative AP-1 and serum response elements, that further increases the serum responsiveness of the Dio3 promoter in a cell-specific manner PMID: 12943987
    27. TGF-beta stimulates transcription of the hDio3 gene via a Smad-dependent pathway. PMID: 16037131
    28. structure and function of the type 3 deiodinase gene [review] PMID: 16131329
    29. postnatal expression of Dio3 can be reactivated in normal tissues during critical illness and other pathologic conditions [review] PMID: 16131330
    30. Deiodinase D3 exists as homo- or heterodimer in living intact cells, a feature that is critical for their catalytic activities. PMID: 18356288
    31. Prx3 knockdown resulted in a 50% decrease in D3-mediated whole-cell deiodination. PMID: 19819956

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  • 亚细胞定位：
    Cell membrane; Single-pass type II membrane protein. Endosome membrane; Single-pass type II membrane protein.
  • 蛋白家族：
    Iodothyronine deiodinase family
  • 组织特异性：
    Expressed in placenta and several fetal tissues.
  • 数据库链接：

    HGNC: 2885

    OMIM: 601038

    KEGG: hsa:1735

    STRING: 9606.ENSP00000427336

    UniGene: Hs.49322