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  4. Recombinant Human Transcription factor 12 (TCF12)

Recombinant Human Transcription factor 12 (TCF12)

  • 中文名称：
    人TCF12重组蛋白
  • 货号：
    CSB-YP023293HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人TCF12重组蛋白
  • 货号：
    CSB-EP023293HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人TCF12重组蛋白
  • 货号：
    CSB-EP023293HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人TCF12重组蛋白
  • 货号：
    CSB-BP023293HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人TCF12重组蛋白
  • 货号：
    CSB-MP023293HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    bHLHb20; Class B basic helix-loop-helix protein 20; DNA binding protein HTF4 ; DNA-binding protein HTF4; E box binding protein; E-box-binding protein; HEB; Helix loop helix transcription factor 4; HsT17266; HTF4; HTF4_HUMAN; TCF-12; Tcf12; Transcription factor 12; Transcription factor HTF-4; Transcription factor HTF4
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-682
  • 氨基酸序列
    MNPQQQRMAA IGTDKELSDL LDFSAMFSPP VNSGKTRPTT LGSSQFSGSG IDERGGTTSW GTSGQPSPSY DSSRGFTDSP HYSDHLNDSR LGAHEGLSPT PFMNSNLMGK TSERGSFSLY SRDTGLPGCQ SSLLRQDLGL GSPAQLSSSG KPGTAYYSFS ATSSRRRPLH DSAALDPLQA KKVRKVPPGL PSSVYAPSPN SDDFNRESPS YPSPKPPTSM FASTFFMQDG THNSSDLWSS SNGMSQPGFG GILGTSTSHM SQSSSYGNLH SHDRLSYPPH SVSPTDINTS LPPMSSFHRG STSSSPYVAA SHTPPINGSD SILGTRGNAA GSSQTGDALG KALASIYSPD HTSSSFPSNP STPVGSPSPL TGTSQWPRPG GQAPSSPSYE NSLHSLQSRM EDRLDRLDDA IHVLRNHAVG PSTSLPAGHS DIHSLLGPSH NAPIGSLNSN YGGSSLVASS RSASMVGTHR EDSVSLNGNH SVLSSTVTTS STDLNHKTQE NYRGGLQSQS GTVVTTEIKT ENKEKDENLH EPPSSDDMKS DDESSQKDIK VSSRGRTSST NEDEDLNPEQ KIEREKERRM ANNARERLRV RDINEAFKEL GRMCQLHLKS EKPQTKLLIL HQAVAVILSL EQQVRERNLN PKAACLKRRE EEKVSAVSAE PPTTLPGTHP GLSETTNPMG HM
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional regulator. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3').
  • 基因功能参考文献：
    1. These results identify HEB as a critical regulator of human mesodermal and hematopoietic specification. PMID: 28803914
    2. HDAC1 promoted migration and invasion of gallbladder tumor cells by binding with TCF12 to promote epithelial mesenchymal transformation. PMID: 27092878
    3. Study describes the identification of three large inherited intragenic exon deletions in TCF12 using whole-genome sequencing and one large inherited duplication using targeted TCF12 sequencing in patients with craniosynostosis. PMID: 27158814
    4. Heb expression is regulated by Med19 in breast cancer cells. PMID: 27572702
    5. enforced expression of transcription factor 12 suppressed cell proliferation, migration, and invasion in vitro and inhibited tumor growth in vivo. In conclusion, transcription factor 12 protein may be a novel molecule which plays a critical role in prostate cancer progression and patients' prognosis, suggesting it might be a promising therapeutic target for prostate cancer therapy PMID: 28651494
    6. HEB may be involved in GBM cell proliferation, as HEB silencing reduced proliferation in cells cultured as monolayers or neurospheres. Furthermore, the results suggested a potential role for HEB in the maintenance of GBM stem cells, as HEB silencing affected the differentiation capacity of cells. PMID: 27779678
    7. Two novel translocations leading to the inactivation of RUNX1 and its partners SIN3A and TCF12 in myeloid leukemia. PMID: 26671595
    8. Studies suggest that transcription factor 12 (TCF12) should be included in level 2 genetic testing. PMID: 25271085
    9. show that these mutations compromise TCF12 transcriptional activity and are associated with a more aggressive tumour type PMID: 26068201
    10. several familial cases of coronal synostosis associated with mutations in TCF12 PMID: 24736737
    11. haploinsufficiency of TCF12 causes coronal synostosis in humans and that severe bilateral coronal synostosis occurs in mice with 50% of the wild-type dosage of both the Tcf12 and Twist1 genes highlights the key role of TCF12 acting with TWIST1. PMID: 23354436
    12. the CD91/IKK/NF-kappaB signaling cascade is involved in secreted HSP90alpha-induced TCF12 expression, leading to E-cadherin down-regulation and enhanced CRC cell migration/invasion PMID: 23386606
    13. TCF12 functioned as a transcriptional repressor of E-cadherin and its overexpression was significantly correlated with the occurrence of CRC metastasis. PMID: 22130667
    14. As a first exploitation of this unique cohort, we identify three novel candidate dyslexia genes, ZNF280D and TCF12 at 15q21, and PDE7B at 6q23.3, by molecular mapping of the familial translocation with the 15q21 breakpoint. PMID: 20798984
    15. T cell development is impaired by Id2, most likely by sequestering HEB, whereas NK cell development is promoted by increasing a pool of CD1a-CD5+ NK cell progenitors, which together with IL-15 differentiate into mature NK cells PMID: 20483740
    16. two alternative acceptor sites for mRNA splicing yield two distinguishable transcripts (HTF4a and HTF4b) which differ in their 5' untranslated region but share identical coding sequences. PMID: 12826747
    17. CBFA2T3 interacts with ZNF652 to repress HEB expression, and in addition CBFA2T3 interacts with the HEB protein to inhibit its activator function. PMID: 18456661
    18. The DNA binding profile of the E-protein HEB was grossly rearranged upon expression of AML1/ETO, and the fusion protein was found to co-localize with both AML1 and HEB on many of its regulated targets PMID: 19043539

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  • 相关疾病：
    Craniosynostosis 3 (CRS3)
  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Expressed in several tissues and cell types including skeletal muscle, thymus, and a B-cell line.
  • 数据库链接：

    HGNC: 11623

    OMIM: 600480

    KEGG: hsa:6938

    STRING: 9606.ENSP00000331057

    UniGene: Hs.511504