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  4. Recombinant Human Transcription factor E2F6 (E2F6)

Recombinant Human Transcription factor E2F6 (E2F6)

  • 中文名称：
    人E2F6重组蛋白
  • 货号：
    CSB-EP007347HU
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    E2F6
  • Uniprot No.：
  • 别名：
    E2F 6; E2F binding site modulating activity protein ; E2F transcription factor 6; E2F transcription factor 6 isoform 1; E2F-6; E2F6; E2F6_HUMAN; EMA; MGC111545; Transcription factor E2F6
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    58.8kDa
  • 表达区域：
    1-281aa
  • 氨基酸序列
    MSQQRPARKLPSLLLDPTEETVRRRCRDPINVEGLLPSKIRINLEDNVQYVSMRKALKVKRPRFDVSLVYLTRKFMDLVRSAPGGILDLNKVATKLGVRKRRVYDITNVLDGIDLVEKKSKNHIRWIGSDLSNFGAVPQQKKLQEELSDLSAMEDALDELIKDCAQQLFELTDDKENERLAYVTYQDIHSIQAFHEQIVIAVKAPAETRLDVPAPREDSITVHIRSTNGPIDVYLCEVEQGQTSNKRSEGVGTSSSESTHPEGPEEEENPQQSEELLEVSN
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal GST-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. Represses expression of some meiosis-specific genes, including SLC25A31/ANT4. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase.
  • 基因功能参考文献：
    1. This analysis showed a relative increase in the expression of E2F6 in gastric adenocarcinoma with no lymph node metastasis (chi (2), P = 0.04 and OR, P = 0.08), while overexpression of RhoA and SMUG1 was found more often in the diffuse subtype of gastric adenocarcinoma as compared to the intestinal subtype. PMID: 27909884
    2. After replication stress, the checkpoint kinase Chk1 phosphorylates E2F6, leading to its dissociation from promoters. This promotes E2F-dependent transcription, which mediates cell survival by preventing DNA damage and cell death PMID: 23954429
    3. findings indicate an inhibitory role of E2F6 in the regulation of IL-13 and allergy PMID: 22981205
    4. E2F6 may recruit BRG1 in transcriptional regulation of genes important for G1/S phase transition of the cell cycle. PMID: 23082233
    5. data suggest that E2F- and Myc-responsive genes are coregulated by E2F6 complex in quiescent cells PMID: 12004135
    6. results suggest that E2F6 represses transcription of the brca1, ctip, art27, hp1alpha, and the rbap48 genes and depletion of E2F6 resulted in the recruitment of E2F1 to the target promoters PMID: 12909625
    7. contains nine exons distributed along 20.4kbp of genomic DNA on chromosome 2 leading to the transcription of six alternatively spliced E2F6 mRNAs that encode four different E2F6 proteins PMID: 15081404
    8. E2F6 does not contain the domains required for modulation of squamous differentiation PMID: 15474455
    9. showed that E2F6, DP1, EPC1, EZH2, and Sin3B co-elute, suggesting the identification of a novel E2F6 complex that exists in vivo in both normal and transformed human cell lines PMID: 15536069
    10. functions as a repressor of E2F-dependent transcription during S phase PMID: 15574595
    11. PHC3 and E2F6 showed nuclear colocalization in a punctate pattern which was not seen in proliferating cells suggesting that PHC3 may be part of an E2F6-polycomb complex that has been shown to occupy and silence target promoters in G(0) PMID: 17001316
    12. E2F-6 inhibited Apaf-1 upregulation by competing with E2F-1 for promoter binding. It enhanced the clonogenic growth of granulocyte, erythroid, macrophage, & megakaryocyte cells.It may prevent hematopoietic progenitor cell loss during proliferation. PMID: 17600109
    13. E2F6 binding sites are located within 2 kb of a transcription start site, in both normal and tumor cells PMID: 17908821

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    E2F/DP family
  • 组织特异性：
    Expressed in all tissues examined. Highest levels in placenta, skeletal muscle, heart, ovary, kidney, small intestine and spleen.
  • 数据库链接：

    HGNC: 3120

    OMIM: 602944

    KEGG: hsa:1876

    STRING: 9606.ENSP00000370936

    UniGene: Hs.603093