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  4. Recombinant Human Transcription factor EB (TFEB)

Recombinant Human Transcription factor EB (TFEB)

  • 货号：
    CSB-YP023429HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP023429HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP023429HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP023429HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP023429HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Alpha TFEB; AlphaTFEB; bHLHe35; Class E basic helix-loop-helix protein 35; T cell transcription factor EB; TCFEB; TFEB; TFEB_HUMAN; Transcription factor EB
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-476
  • 氨基酸序列
    MASRIGLRMQ LMREQAQQEE QRERMQQQAV MHYMQQQQQQ QQQQLGGPPT PAINTPVHFQ SPPPVPGEVL KVQSYLENPT SYHLQQSQHQ KVREYLSETY GNKFAAHISP AQGSPKPPPA ASPGVRAGHV LSSSAGNSAP NSPMAMLHIG SNPERELDDV IDNIMRLDDV LGYINPEMQM PNTLPLSSSH LNVYSSDPQV TASLVGVTSS SCPADLTQKR ELTDAESRAL AKERQKKDNH NLIERRRRFN INDRIKELGM LIPKANDLDV RWNKGTILKA SVDYIRRMQK DLQKSRELEN HSRRLEMTNK QLWLRIQELE MQARVHGLPT TSPSGMNMAE LAQQVVKQEL PSEEGPGEAL MLGAEVPDPE PLPALPPQAP LPLPTQPPSP FHHLDFSHSL SFGGREDEGP PGYPEPLAPG HGSPFPSLSK KDLDLMLLDD SLLPLASDPL LSTMSPEASK ASSRRSSFSM EEGDVL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor that acts as a master regulator of lysosomal biogenesis, autophagy, lysosomal exocytosis, lipid catabolism, energy metabolism and immune response. Specifically recognizes and binds E-box sequences (5'-CANNTG-3'); efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. Involved in the cellular response to amino acid availability by acting downstream of MTOR: in the presence of nutrients, TFEB phosphorylation by MTOR promotes its cytosolic retention and subsequent inactivation. Upon starvation or lysosomal stress, inhibition of MTOR induces TFEB dephosphorylation, resulting in nuclear localization and transcription factor activity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression, thereby playing a central role in expression of lysosomal genes. Regulates lysosomal positioning in response to nutrient deprivation by promoting the expression of PIP4P1. Acts as a positive regulator of autophagy by promoting expression of genes involved in autophagy. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta. Involved in the immune response to infection by the bacteria S.aureus or S.enterica, acting downstream of protein kinase D (PKD), probably by regulating cytokine and chemokine expression.
  • 基因功能参考文献：
    1. TMEM106B drives lung cancer metastasis by inducing TFEB-dependent lysosome synthesis and secretion of cathepsins. PMID: 30013069
    2. data on TFEB nucleo-cytoplasmic shuttling suggest an unpredicted role of mTOR in nuclear export. PMID: 30120233
    3. The transcription factor EB (TFEB) nuclear export signal (NES) integrates carbon (glucose) and nitrogen (amino acid) availability by controlling TFEB flux through a nuclear import-export cycle. PMID: 29992949
    4. TFEB knockdown reduces invasion and migration of cancer cells, likely through lysosomal regulation. Taken together, TFEB influences cell invasion and migration of oral squamous cell carcinomas. PMID: 29316035
    5. Akt phosphorylates TFEB at Ser467 and represses TFEB nuclear translocation independently of mechanistic target of rapamycin complex 1, a known TFEB inhibitor. Pharmacological inhibition of Akt promotes cellular clearance in cells from patients with a variety of lysosomal diseases. PMID: 28165011
    6. these data suggest that the TFEB/TMEM55B/JIP4 pathway coordinates lysosome movement in response to a variety of stress conditions. PMID: 29146937
    7. Data suggest that the effects of Alpha-TFEB gene fusion are specific in renal cell carcinoma, which results in the overexpression of a native TFEB protein and then promotes cell canceration. PMID: 29328409
    8. MAP4K3 is identified as an amino acid-dependent regulator of autophagy through its phosphorylation of transcription factor EB (TFEB), a transcriptional activator of autophagy. PMID: 29507340
    9. Overexpression of TFEB is associated with Breast Cancer. PMID: 28017540
    10. Results identify cigarette smoke (CS)-induced accumulation of TFEB in aggresome-bodies as a specific novel mechanism for CS-mediated autophagy-impairment and resulting aggresome-formation and emphysema progression. PMID: 27835930
    11. This study reveals a critical link between two keys factors in tumourigenesis and autophagy, and suggests a potential important role of p53-TFEB signaling axis in lung cancer. PMID: 28292013
    12. Taken together, these data demonstrate that ATO induces osteosarcoma cell death via inducing excessive autophagy, which is mediated through the ROS-TFEB pathway. The present study provides a new anti-tumor mechanism of ATO treatment in osteosarcoma. PMID: 29307831
    13. cellular vacuolization, represents a condition of profound lysosome stress, and cells sense and respond to this stress by facilitating mTOR-dependent TFEB nucleus translocation in an effort to restore lysosome homeostasis. PMID: 27593892
    14. TFEB and TFE3 collaborate with each other in activated macrophages and microglia to promote efficient autophagy induction, increased lysosomal biogenesis, and transcriptional upregulation of numerous proinflammatory cytokines PMID: 27171064
    15. TFEB is affected by a novel curcumin analog in vitro and in vivo independent of MTOR inhibition PMID: 27172265
    16. TFEB regulates PER3 expression via glucose-dependent effects on CLOCK/BMAL1 PMID: 27373683
    17. These data reveal a novel mechanism of TFEB regulation by MTORC1 essential for lysosomal biogenesis. PMID: 28055300
    18. Data suggest that transcription factor EB (TFEB) is a protective transcription factor against endothelial cell inflammation and a potential target for treating atherosclerosis and associated cardiovascular diseases. PMID: 28143903
    19. Both Danon Disease and glycogen storage disease type II show accumulation and altered localization of VPS15 in autophagy-incompetent fibers. However, TFEB displays a different pattern between these two lysosomal storage diseases PMID: 28102838
    20. Studies identified TFEB and TFE3 as master modulators of stress response notably in the lysosomal biogenesis and autophagy with capability to upregulate hundreds of genes involved in intracellular clearance, catabolism, metabolic processes, and cellular homeostasis. PMID: 27892768
    21. Findings suggest that TFEB activation in tauopathy model mice stimulates the autophagy-lysosome pathway, resulting in the clearance of PHF-tau and lipofuscins, which in turn may rescue loss of synapses, and learning and memory deficits. PMID: 27257626
    22. Data suggest that PEG3 is required for TFEB induction and nuclear translocation in a VEGFR2- and AMPK-dependent manner for decorin/decorin receptor-evoked autophagy. (PEG3 = paternally expressed 3 protein; TFEB = transcription factor EB; VEGFR2 = vascular endothelial growth factor receptor-2; AMPK = AMP-activated protein kinase) PMID: 28798237
    23. review of role linking cellular stress to immune response PMID: 28656016
    24. Results demonstrate that TFEB levels and subcellular distribution undergo distinct short-term and long-term control. These findings suggest that the rapid rheostatic response, mediated by mTOR, allows the cell to quickly adapt to metabolic changes, while the long-term, mTOR independent homeostatic response controls the magnitude and duration of TFEB activation, and presumably limits excessive autophagy. PMID: 27268034
    25. Consistent with reduced transcription factor EB (TFEB) activity, accumulation of phosphorylated TFEB in STUB1-deficient cells resulted in reduced autophagy and reduced mitochondrial biogenesis. These studies reveal that the ubiquitin-proteasome pathway participates in regulating autophagy and lysosomal functions by regulating the activity of TFEB. PMID: 28754656
    26. TFEB has attracted a lot of attention owing to its ability to induce the intracellular clearance of pathogenic factors in a variety of murine models of disease, such as Parkinson's and Alzheimer's, suggesting that novel therapeutic strategies could be based on the modulation of TFEB activity. PMID: 27252382
    27. Amplification of chromosome 6p including the TFEB gene is a novel occurrence in renal cell carcinoma, which seems to be associated with an often aggressive and infiltrative tubulopapillary growth pattern. PMID: 28009604
    28. TFEB-amplified renal cell carcinomas represent a distinct molecular subtype of high-grade adult renal cell carcinomas associated with aggressive clinical behavior, variable morphology, and aberrant melanocytic marker expression PMID: 27565001
    29. Case Report: suggest that extensive sclerosis and ossification may be a less common recurring histology of TFEB-rearrangement renal cell carcinoma. PMID: 27864122
    30. Overexpression of deacetylated transcription factor EB at K116R mutant in microglia accelerated intracellular fibrillar Amyloid beta-peptide degradation by stimulating lysosomal biogenesis and greatly reduced the deposited amyloid plaques in the brain. PMID: 27209302
    31. the central autophagy regulator TFEB is expressed and active in PDAC, but autophagy is sustained after TFEB knockdown, suggesting alternative bypass signaling. TFEB is dispensable for gemcitabine-induced cell death, but inversely correlated with KRAS expression. PMID: 27175909
    32. Neuronal C-ETS2 senses oxidative stress, activates TFEB transcription, and mediates the upregulation of lysosomal genes. PMID: 27195074
    33. TFEB is phosphorylated and inactivated by Akt. PMID: 28165011
    34. Activation of TFEB ameliorates disease phenotypes in a mouse model of Batten disease. PMID: 28165011
    35. Lack of cystinosin reduced TFEB expression and induced TFEB nuclear translocation. PMID: 26994576
    36. The autophagic response to polystyrene nanoparticles is mediated by TFEB and depends on surface charge. PMID: 26596266
    37. TFEB1 overexpression is associated with drug resistance of ovarian cancer. PMID: 26307679
    38. Review of the role of TFEB in lysosome biogenesis, transcriptional and post-transcriptional regulation of TFEB, and TFEB involvement in neurodegenerative disease as a dysregulated factor or as an agent used to promote cellular clearance. PMID: 27299292
    39. TFEB and TFE3 are novel components of the integrated stress response PMID: 26813791
    40. Silencing of TFEB with siRNAs in lung cancer cell lines resulted in reduced migration ability. PMID: 26264650
    41. during mitophagy TFEB translocates to the nucleus and displays transcriptional activity in a PINK1- and Parkin-dependent manner. PMID: 26240184
    42. a virus modulating TFEB localization and helps to explain how HIV modulates autophagy to promote its own replication and cell survival PMID: 26115100
    43. RIP1 represses basal autophagy in part due to its ability to regulate the TFEB transcription factor;RIP1 activates ERK, which negatively regulates TFEB though phosphorylation of serine 142 PMID: 25908842
    44. TFEB modulates autophagic clearance of alpha-syn PMID: 25790376
    45. Data show that drug-induced TFEB-associated lysosomal biogenesis is a determinant of multidrug resistance (MDR) and suggest that circumvention of lysosomal drug sequestration is a strategy to overcome chemoresistance. PMID: 25544758
    46. This study demonstrated that transcription factor EB (TFEB) regulate the lysosome biogenesis in neurons of APP/PS1 mice, steady-state levels of APP were reduced, resulting in decreased interstitial fluid Abeta levels and attenuated amyloid deposits PMID: 26338325
    47. TFEB was found to regulate MuRF1 expression in Angiotensin II-induced skeletal muscle atrophy. PMID: 26137861
    48. Review of the role of gene fusions involving TFE3 and TFEB in carcinogenesis in sporadic renal cell carcinoma. PMID: 25048860
    49. Results showed the amplification of TFEB locus was found only in the aggressive t(6;11) Renal Cell Carcinoma. PMID: 25438924
    50. Lysosomal calcium signaling regulates autophagy through calcineurin and TFEB. PMID: 25720963

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  • 亚细胞定位：
    Cytoplasm, cytosol. Lysosome membrane. Nucleus.; Nucleus.
  • 蛋白家族：
    MiT/TFE family
  • 数据库链接：

    HGNC: 11753

    OMIM: 600744

    KEGG: hsa:7942

    STRING: 9606.ENSP00000230323

    UniGene: Hs.485360