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  4. Recombinant Human Transcription factor Sp7 (SP7)

Recombinant Human Transcription factor Sp7 (SP7)

  • 中文名称：
    人SP7重组蛋白
  • 货号：
    CSB-YP851549HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人SP7重组蛋白
  • 货号：
    CSB-EP851549HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人SP7重组蛋白
  • 货号：
    CSB-EP851549HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人SP7重组蛋白
  • 货号：
    CSB-BP851549HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人SP7重组蛋白
  • 货号：
    CSB-MP851549HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SP7
  • Uniprot No.：
  • 别名：
    MGC126598; Osterix; Osx; Sp 7; SP7; Sp7 transcription factor; SP7_HUMAN; Transcription factor Sp7; Zinc finger protein osterix
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-431
  • 氨基酸序列
    MASSLLEEEV HYGSSPLAML TAACSKFGGS SPLRDSTTLG KAGTKKPYSV GSDLSASKTM GDAYPAPFTS TNGLLSPAGS PPAPTSGYAN DYPPFSHSFP GPTGTQDPGL LVPKGHSSSD CLPSVYTSLD MTHPYGSWYK AGIHAGISPG PGNTPTPWWD MHPGGNWLGG GQGQGDGLQG TLPTGPAQPP LNPQLPTYPS DFAPLNPAPY PAPHLLQPGP QHVLPQDVYK PKAVGNSGQL EGSGGAKPPR GASTGGSGGY GGSGAGRSSC DCPNCQELER LGAAAAGLRK KPIHSCHIPG CGKVYGKASH LKAHLRWHTG ERPFVCNWLF CGKRFTRSDE LERHVRTHTR EKKFTCLLCS KRFTRSDHLS KHQRTHGEPG PGPPPSGPKE LGEGRSTGEE EASQTPRPSA SPATPEKAPG GSPEQSNLLE I
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional activator essential for osteoblast differentiation. Binds to SP1 and EKLF consensus sequences and to other G/C-rich sequences.
  • 基因功能参考文献：
    1. Data support a model wherein p53 represses OSX-DNA binding and DLX5-OSX interaction, and thereby deregulates the osteogenic transcriptional network. PMID: 28777372
    2. Results showed that blocking miR-31 led to an increase in osterix protein in both cell types at day 7, with an increase in osteocalcin at day 21, suggesting MSC osteogenesis PMID: 29444183
    3. Results provide evidence that CBP-mediated acetylation and HDAC4-mediated deacetylation have critical roles in the modification of Osx, and thus are important in osteoblast differentiation. PMID: 27250035
    4. Osterix and RUNX2 are transcriptional regulators of sclerostin in human bone PMID: 27154028
    5. Osterix decreased the chemosensitivity of breast cancer cells by upregulating the expression of GALNT14, which eventually suppressed the apoptosis of breast cancer cells. PMID: 29227978
    6. TP(thymidine phosphorylase ) curbed the expression of three proteins-IRF8, RUNX2, and osterix. This downregulation was epigenetically driven: High levels of 2DDR, a product of TP secreted by myeloma cells, activated PI3K/AKT signaling and increased the methyltransferase DNMT3A's expression PMID: 27658717
    7. dissection of these interconnected epigenetic mechanisms that govern Sp7 gene activation reveals a hierarchical process where regulatory components mediating DNA demethylation play a leading role PMID: 28784721
    8. SP7 gene promoter is robustly enriched in epigenetic repressive marks that may explain its poor transcriptional response to osteoblast differentiating media in umbilical cord derived mesenchymal stem cells. PMID: 27689934
    9. Eight and five of the nine samples were negative for cell adhesion molecule 1 and Osterix respectively. The other markers showed no statistical significance(CD151,ALP). osteoblastic differentiation can occur in carcinoma cells and that cell adhesion molecule 1 could be a useful marker for identifying this phenomenon in carcinoma tissues PMID: 28651491
    10. The results suggest that Osterix plays an important role in increasing BMP- 4-induced Cx43 activity. PMID: 27498006
    11. The expression of specific targets Smad1 and Osterix was significantly increased in the presence of Pi and restored by coincubation with Mg(2+). As miR-30b, miR-133a, and miR-143 are negatively regulated by Pi and restored by Mg(2+) with a congruent modulation of their known targets Runx2, Smad1, and Osterix, our results provide a potential mechanistic explanation of the observed upregulation of these master switches of o PMID: 27419135
    12. Preameloblast-Derived Factors Mediate Osteoblast Differentiation of Human Bone Marrow Mesenchymal Stem Cells by Runx2-Osterix-BSP Signaling. PMID: 26413977
    13. Osx might function as a potential regulator for the proliferation and odontoblastic differentiation of hDPCs. PMID: 25258338
    14. Data suggest that beta-catenin (beta-cat) signaling upregulates the expression of osterix (OSX) in pre-osteoblastic and bone marrow stromal cells. PMID: 26496941
    15. The 2 genes RUNX1 and SP7 resulted differently expressed in cells cultured on metallic supports if compared with the expression recorded for OIC PMID: 25025858
    16. c-Src signaling modulates osteoblast differentiation at least in part through phosphorylation of Osterix. PMID: 25802190
    17. Pin1 regulates the osteogenic activity of Osterix. PMID: 25463757
    18. Runx2-Sp7 molecular complex functionally cooperate for maximal induction of cell-phenotype-restricted genes PMID: 25158187
    19. Osterix is a novel target of protein kinase A, and protein kinase A modulates osteoblast differentiation partially through the regulation of Osterix. PMID: 24905700
    20. results provide a molecular description of a mechanism for Osx and Runx2 transcriptional cooperation that is subject to further regulation by MAPK-activating signals during osteogenesis. PMID: 25122769
    21. In adamantinomatous craniopharyngioma, Bmp2 was expressed primarily in the stellate reticulum and whorl-like array cells; Runx2 and Osterix tended to be expressed in calcification-related epithelia. PMID: 24387671
    22. MiR-31, controls Osterix expression through association to the 3' untranslated region of this transcription factor. PMID: 23827457
    23. These results suggest that Osterix is a novel target of CaMKII and the activity of Osterix can be modulated by a novel mechanism involving CaMKII during osteoblast differentiation. PMID: 23402759
    24. Osx is an unstable protein, and that the ubiquitin-proteasome pathway is involved in the regulation of Osx and thereby regulates osteoblast differentiation PMID: 23457570
    25. Consensus Sp1 sequences, located in the proximal promoter and in the bone-enhancer, as Osterix binding regions in the Col1a1 promoter in vitro and in vivo, were identified. PMID: 23159876
    26. Osterix expression in fibroblasts was induced by fluoride, which could play an important role in the development of fluoride bone injuries and ossification around bone. PMID: 21351630
    27. Osterix regulates calcification and degradation of chondrogenic matrices through matrix metalloproteinase 13 (MMP13) expression in association with transcription factor Runx2 during endochondral ossification PMID: 22869368
    28. miR-93/Sp7 function loop mediates osteoblast mineralization PMID: 22467200
    29. Osterix regulates the expression of a set of extracellular matrix proteins which are involved in BMP-induced terminal osteoblast differentiation. PMID: 21898406
    30. Results suggest that Osterix is a direct transcriptional regulator with repressive effect on NELL-1 gene expression, contributing to a delicate balance of regulatory effects on NELL-1 transcription with Runx2. PMID: 21931789
    31. Osterix, a significant transcription factor of osteoblasts, was shown to be a direct target of miR-637 PMID: 21880893
    32. These results suggest that Erk1/2 regulates a major transcription factor, Osterix, during osteoblast differentiation by increasing its protein stability and transcriptional activity. PMID: 22056560
    33. RT-PCR showed that SP7 from osteoblasts from Pfeiffer syndrome grown on PLPG acid plates were downregulated after 30 days. PMID: 21558934
    34. Akt activity enhances the osteogenic function of Osterix, at least in part, through protein stabilization and that BMP-2 regulates the osteogenic function of Osterix, at least in part, through Akt. PMID: 21777568
    35. Evidence from a genome-wide association study (in USA; in pts of European descent) indicate that an SNP at one locus, Osterix, is associated with childhood obesity; G allele of rs2016266 is significantly over-represented in obesity but only in girls. PMID: 21212767
    36. Sox9, Runx2, and Osterix-were play an essential role in determining the skeletal progenitor cells' fate in benign cartilage and bone forming tumors. PMID: 21078438
    37. The presence of NFATc1 and Osx in our material lends further support to the hypothesis that during the process of aortic valve calcification there is expression of osteoblastic phenotypes by valvular cells PMID: 19019468
    38. Telomerase accelerates osteogenesis of bone marrow stromal stem cells by upregulation of CBFA1, osterix, and osteocalcin. PMID: 12674332
    39. Sp7 expression in humans is largely confined to osteoblasts and chondrocytes, both of which differentiate from the mesenchymal lineage. Of the two protein isoforms, the short isoform is most abundant. PMID: 14604442
    40. The identification and initial characterization of the SP7 gene will facilitate the study of the molecular regulation of osteoblast differentiation in humans. PMID: 15474293
    41. Gene expression of osterix was not detected in dental follicle cells during osteogenic differentiation. PMID: 16467978
    42. results indicated that pcDNA3.1-Osx transfection promoted the osteogenic differentiation of adipose derived stem cells, while not affecting their proliferative ability PMID: 17206379
    43. identified new Osterix interacting factors by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry PMID: 17303075
    44. osterix functions as a molecular link between mechanostressing and osteogenic differentiation PMID: 17311298
    45. These results demonstrate the transcriptional regulation of Osterix expression by Runx3 during differentiation of dental pulp cells into odontoblasts during tooth development. PMID: 17352693
    46. might play an important role in the mechanical stress-induced osteogenic differentiation of periodontal ligament cells PMID: 18471237
    47. This is the first report demonstrating significant upregulation in expression of Runx2 and Osterix by TGF-beta3 induction of human adipose-derived stromal cells during in vitro chondrogenesis. PMID: 18482578
    48. dried plum polyphenols enhance osteoblast activity and function by attenuating the effect of TNFalpha and up-regulating Runx2, Osterix and IGF-I and increasing lysyl oxidase expression, and at the same time attenuate osteoclastogenesis signaling PMID: 18495459
    49. This study further confirms that the involvement of SP7 in bone physiology is not only limited to the developmental step. PMID: 18777089
    50. E4BP4 has a role as osteoblast transcriptional repressor in inhibiting both Runx2 and Osterix in myeloma bone disease PMID: 18829486

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  • 相关疾病：
    Osteogenesis imperfecta 12 (OI12)
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Sp1 C2H2-type zinc-finger protein family
  • 组织特异性：
    Restricted to bone-derived cell.
  • 数据库链接：

    HGNC: 17321

    OMIM: 606633

    KEGG: hsa:121340

    STRING: 9606.ENSP00000302812

    UniGene: Hs.209402