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  4. Recombinant Human Transcription initiation factor IIB (GTF2B)

Recombinant Human Transcription initiation factor IIB (GTF2B)

  • 货号：
    CSB-EP010001HU
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    GTF2B
  • Uniprot No.：
  • 别名：
    General transcription factor IIB; General transcription factor TFIIB; gtf2b; RNA polymerase II transcription factor IIB; S300 II; S300-II; TF IIB; TF2B; TF2B_HUMAN; TFIIB; Transcription initiation factor IIB
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    50.8kDa
  • 表达区域：
    1-316aa
  • 氨基酸序列
    MASTSRLDALPRVTCPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLITTGDFMSRFCSNLCLPKQVQMAATHIARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYRLIYPRAPDLFPTDFKFDTPVDKLPQL
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA. Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex. Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle. Associates with chromatin to core promoter-specific regions. Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner; these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-[GC][GC][GA]CGCC-3', and downstream (BREd), 5'-[GA]T[TGA][TG][GT][TG][TG]-3', of the TATA box element. Modulates transcription start site selection. Exhibits also autoacetyltransferase activity that contributes to the activated transcription.
  • 基因功能参考文献：
    1. a hand-off model in which Ssu72 mediates a conformational transition in TFIIB, accounting for the role of Ssu72 in transcription reinitiation, gene looping, and promoter-terminator cross-talk. PMID: 29158257
    2. binding of specific DNA sequences changes the protein structure and dynamics, and TFIIB may exist in two conformational states PMID: 26284261
    3. The role of zinc at Cys3His1 binding site in the absence of TFIIB protein folding PMID: 26016528
    4. these results establish a new paradigm for TFIIB functionality in human gene expression, which when downregulated has potent anti-viral effects. PMID: 24441171
    5. Association of the winged helix motif of the TFIIEalpha subunit of TFIIE with either the TFIIEbeta subunit or TFIIB distinguishes its functions in transcription. PMID: 25492609
    6. TFIIB was involved in the proliferation and growth of HCC cells. PMID: 23055019
    7. a mode of phospho-TFIIB-independent transcriptional regulation that prioritizes the transcription of p53-target genes during cellular stress. PMID: 23115335
    8. Data show that TFIIF has an important role in stabilizing TFIIB within the PIC and after transcription initiates. PMID: 21896726
    9. a six-amino acid human TFIIB tip region is needed for appropriate levels of serine 5 C-terminal domain phosphorylation and mRNA capping and for retention of the required elongation factor TFIIF. PMID: 20880846
    10. phosphorylation of TFIIB at serine 65 is a critical event in transcription that links the gene promoter and terminator and triggers initiation by RNA pol II PMID: 20226668
    11. interaction with vitamin D receptor helix H10 residues PMID: 12529369
    12. Data show that human TATA box binding protein (TBP) can use a shared surface to interact with two different transcription factor IIB (TFIIB) family members to initiate transcription by different RNA polymerases. PMID: 12535529
    13. The groove in the first cyclin repeat of TFIIB is identified as the FCP1-binding site of TFIIB core domain (TFIIBc), consisting of several hydrophobic and several basic amino acids. PMID: 12578358
    14. lack of role in bending of TATA-binding protein simultaneously with promotoer DNA PMID: 12791683
    15. TFIIB interacts with SP1/SP3 at the SP1 site, besides its association with EAR3 and the TATA-less core promoter region. PMID: 12972613
    16. in addition to its role in regulating TBP binding to a TATA box, the TBP surface is unexpectedly involved in TBP association with all three TFIIB family members PMID: 14585974
    17. The structural role of Zn2+ binding to the the zinc-ribbon domain of TFIIB was analysed. PMID: 14641108
    18. Results identify a small nonconserved surface of the transcription factor TFIIB zinc ribbon that is required for RNA polymerase II (pol II) transcription in vivo and for different types of basal pol II transcription in vitro. PMID: 15024075
    19. A TFIIB conformational change is critical for the formation of activator-dependent transcription complexes. PMID: 15037660
    20. Collapse of the transcription bubble defines the RNA polymerase II promoter clearance transition. PMID: 15989968
    21. high level of TFIIB can bypass the Mediator requirement for basal transcription and pol II recruitment in nuclear extract PMID: 16595664
    22. Together, our results support a mode of preinitiation complex assembly in which TFIIB/RNA polymerase II recruitment to the promoter occurs in vivo. PMID: 16878124
    23. TFIIB binding is required for the interferon antagonist effect exerted by Thogoto virus M protein. PMID: 18768974
    24. slipping at juncture is induced by TATA-binding protein and transcription factor IIB and requires a TATA box but not a transcription factor IIB recognition sequence PMID: 19193635
    25. TFIIF in HeLa cell nuclear extract helps to rescue the inactive mutations by interacting with either the B-finger or another component of the initiation complex that is influenced by the B-finger PMID: 19590095

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  • 亚细胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    TFIIB family
  • 组织特异性：
    Expressed in the inner cell mass forming the embryoblast. Not detected in cells from the outer thin layer trophoblast (at protein level).
  • 数据库链接：

    HGNC: 4648

    OMIM: 189963

    KEGG: hsa:2959

    STRING: 9606.ENSP00000359531

    UniGene: Hs.481852