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  4. Recombinant Human Transcriptional activator protein Pur-beta (PURB)

Recombinant Human Transcriptional activator protein Pur-beta (PURB)

  • 中文名称：
    人PURB重组蛋白
  • 货号：
    CSB-YP847212HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人PURB重组蛋白
  • 货号：
    CSB-EP847212HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人PURB重组蛋白
  • 货号：
    CSB-EP847212HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人PURB重组蛋白
  • 货号：
    CSB-BP847212HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人PURB重组蛋白
  • 货号：
    CSB-MP847212HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PURB
  • Uniprot No.：
  • 别名：
    Pur beta; purB; PURB_HUMAN; PURBETA; Purine rich element binding protein B; Purine-rich element-binding protein B; transcriptional activator protein Pur beta; Transcriptional activator protein Pur-beta
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-312
  • 氨基酸序列
    ADGDSGSER GGGGGPCGFQ PASRGGGEQE TQELASKRLD IQNKRFYLDV KQNAKGRFLK IAEVGAGGSK SRLTLSMAVA AEFRDSLGDF IEHYAQLGPS SPEQLAAGAE EGGGPRRALK SEFLVRENRK YYLDLKENQR GRFLRIRQTV NRGGGGFGAG PGPGGLQSGQ TIALPAQGLI EFRDALAKLI DDYGGEDDEL AGGPGGGAGG PGGGLYGELP EGTSITVDSK RFFFDVGCNK YGVFLRVSEV KPSYRNAITV PFKAWGKFGG AFCRYADEMK EIQERQRDKL YERRGGGSGG GEESEGEEVD ED
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Has capacity to bind repeated elements in single-stranded DNA such as the purine-rich single strand of the PUR element located upstream of the MYC gene. Plays a role in the control of vascular smooth muscle (VSM) alpha-actin gene transcription as repressor in myoblasts and fibroblasts. Participates in transcriptional and translational regulation of alpha-MHC expression in cardiac myocytes by binding to the purine-rich negative regulatory (PNR) element. Modulates constitutive liver galectin-3 gene transcription by binding to its promoter. May play a role in the dendritic transport of a subset of mRNAs.
  • 基因功能参考文献：
    1. Results showed that Purbeta ssDNA-binding activity was significantly increased in myeloid cells from AML patients suggesting that the enhanced ssDNA-binding activity of Purbeta in transformed myeloid cells may serve as a unique and measurable phenotypic trait for improving prognostic risk stratification in AML. PMID: 28834593
    2. site-directed mutagenesis revealed several basic amino acid residues in the intermolecular (R267) and intramolecular (K82 and R159) subdomains that are essential for Purbeta transcriptional repressor function in Acta2 promoter-reporter assays. In keeping with their diminished Acta2 repressor activity in fibroblasts, purified Purbeta variants containing an R267A mutation exhibited reduced binding affinity for purine-ric... PMID: 27064749
    3. findings offer insights into mechanism, energetics, and sequence of Purbeta repressor binding to a biologically relevant, contractile phenotype-regulating cis-element while revealing the thermodynamic confines of Purbeta-mediated effects on DNA structure PMID: 19496623
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    PUR DNA-binding protein family
  • 组织特异性：
    Expressed in myocardium of heart failure patients.
  • 数据库链接：

    HGNC: 9702

    OMIM: 608887

    KEGG: hsa:5814

    STRING: 9606.ENSP00000379051

    UniGene: Hs.349150