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  4. Recombinant Human Transforming growth factor beta activator LRRC33 (NRROS), partial

Recombinant Human Transforming growth factor beta activator LRRC33 (NRROS), partial

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  • 中文名称：
    人NRROS重组蛋白
  • 货号：
    CSB-EP801264HU
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP801264HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) NRROS.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP801264HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) NRROS.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    NRROS
  • Uniprot No.：
  • 别名：
    NRROS; LRRC33; UNQ3030/PRO9833Transforming growth factor beta activator LRRC33; Leucine-rich repeat-containing protein 33; Negative regulator of reactive oxygen species
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 来源：
    E.coli
  • 分子量：
    74.5 kDa
  • 表达区域：
    19-650aa
  • 氨基酸序列
    WRNRSGTATAASQGVCKLVGGAADCRGQSLASVPSSLPPHARMLTLDANPLKTLWNHSLQPYPLLESLSLHSCHLERISRGAFQEQGHLRSLVLGDNCLSENYEETAAALHALPGLRRLDLSGNALTEDMAALMLQNLSSLRSVSLAGNTIMRLDDSVFEGLERLRELDLQRNYIFEIEGGAFDGLAELRHLNLAFNNLPCIVDFGLTRLRVLNVSYNVLEWFLATGGEAAFELETLDLSHNQLLFFPLLPQYSKLRTLLLRDNNMGFYRDLYNTSSPREMVAQFLLVDGNVTNITTVSLWEEFSSSDLADLRFLDMSQNQFQYLPDGFLRKMPSLSHLNLHQNCLMTLHIREHEPPGALTELDLSHNQLSELHLAPGLASCLGSLRLFNLSSNQLLGVPPGLFANARNITTLDMSHNQISLCPLPAASDRVGPPSCVDFRNMASLRSLSLEGCGLGALPDCPFQGTSLTYLDLSSNWGVLNGSLAPLQDVAPMLQVLSLRNMGLHSSFMALDFSGFGNLRDLDLSGNCLTTFPRFGGSLALETLDLRRNSLTALPQKAVSEQLSRGLRTIYLSQNPYDCCGVDGWGALQHGQTVADWAMVTCNLSSKIIRVTELPGGVPRDCKWERLDLGL
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The production of this recombinant Human NRROS protein is just like all recombinant proteins. The process involved transfecting E.coli cells with DNA vector containing the template of recombinant DNA. The E.coli cells containing the template were then cultured so that they could transcribe and translate the NRROS protein. N-terminal 10xHis tag & C-terminal Myc tag was used in the process. The purity is 85% determined by SDS-PAGE.

    The NRROS gene provides instructions for making a protein called transforming growth factor-beta activator LRRC33(also named leucine-rich repeat-containing protein 33Curated or negative regulator of reactive oxygen species). LRRC33 protein is a single-pass transmembrane protein involved immune response, superoxide metabolic process and transforming growth factor-beta receptor signaling pathway. LRRC33, a homologous protein of the pro-TGF-β1 binding protein GARP (LRRC32), is covalently linked to the prodomain of TGF-β1, and highly expressed microglial cells in the central nervous system (CNS).

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Key regulator of transforming growth factor beta-1 (TGFB1) specifically required for microglia function in the nervous system. Required for activation of latent TGF-beta-1 in macrophages and microglia: associates specifically via disulfide bonds with the Latency-associated peptide (LAP), which is the regulatory chain of TGFB1, and regulates integrin-dependent activation of TGF-beta-1. TGF-beta-1 activation mediated by LRRC33/NRROS is highly localized: there is little spreading of TGF-beta-1 activated from one microglial cell to neighboring microglia, suggesting the existence of localized and selective activation of TGF-beta-1 by LRRC33/NRROS. Indirectly plays a role in Toll-like receptor (TLR) signaling: ability to inhibit TLR-mediated NF-kappa-B activation and cytokine production is probably a consequence of its role in TGF-beta-1 signaling.
  • 基因功能参考文献：
    1. results suggest that LRRC33 and TLRs may form a negative-feedback loop, which is important for the maintenance of immune homeostasis. PMID: 24550525
    2. LRRC33 represents the first identified LRR-containing protein that plays a critical role in desensitizing Toll-like receptor (TLR)-mediated innate immune responses. PMID: 23545260
  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein.
  • 组织特异性：
    Mainly expressed in cells of hematopoietic origin. Highly expressed in bone marrow, thymus, liver, lung, intestine and spleen. In the brain, highly expressed in microglia.
  • 数据库链接：

    HGNC: 24613

    OMIM: 615322

    KEGG: hsa:375387

    STRING: 9606.ENSP00000328625

    UniGene: Hs.702186