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  4. Recombinant Human Tripartite motif-containing protein 72 (TRIM72), partial

Recombinant Human Tripartite motif-containing protein 72 (TRIM72), partial

In Stock
  • 货号：
    CSB-EP744394HU1
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    TRIM72
  • Uniprot No.：
  • 别名：
    LOC493829; Mg53; Mitsugumin-53; TRI72_HUMAN; trim72; Tripartite motif containing 72; Tripartite motif containing protein 72 ; Tripartite motif-containing protein 72
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 来源：
    E.coli
  • 分子量：
    79.4 kDa
  • 表达区域：
    2-477aa
  • 氨基酸序列
    SAAPGLLHQELSCPLCLQLFDAPVTAECGHSFCRACLGRVAGEPAADGTVLCPCCQAPTRPQALSTNLQLARLVEGLAQVPQGHCEEHLDPLSIYCEQDRALVCGVCASLGSHRGHRLLPAAEAHARLKTQLPQQKLQLQEACMRKEKSVAVLEHQLVEVEETVRQFRGAVGEQLGKMRVFLAALEGSLDREAERVRGEAGVALRRELGSLNSYLEQLRQMEKVLEEVADKPQTEFLMKYCLVTSRLQKILAESPPPARLDIQLPIISDDFKFQVWRKMFRALMPALEELTFDPSSAHPSLVVSSSGRRVECSEQKAPPAGEDPRQFDKAVAVVAHQQLSEGEHYWEVDVGDKPRWALGVIAAEAPRRGRLHAVPSQGLWLLGLREGKILEAHVEAKEPRALRSPERRPTRIGLYLSFGDGVLSFYDASDADALVPLFAFHERLPRPVYPFFDVCWHDKGKNAQPLLLVGPEGAEA
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal GST-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer，50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The synthesis of this Recombinant Human TRIM72 protein depends on the utilization of recombinant DNA technology to a large degree. DNA sequences that encoded the TRIM72 protein could be inserted into a vector and introduced into an expression host, E.coli, where it could be easily expressed in and purified from. The expression of this TRIM72 protein was at 2-477aa. N-terminal GST tag was fused with this protein. The purity is 85%+ determined by SDS-PAGE.

    TRIM72 (also known as MG53) is gene encoding a protein named tripartite motif-containing protein 72 (TRIM72) or mitsugumin-53 (Mg53) in human. The protein encoded by this gene is a muscle-specific TRIM family protein. This protein is initially identified as a key regulator of cell membrane repair machinery. It has been suggested to be a critical regulator of muscle insulin signaling pathway by acting as ubiquitin E3 ligase targeting both the insulin receptor and insulin receptor substrate 1 (IRS1).

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles.
  • 基因功能参考文献：
    1. serum TRIM72 may be a potential biomarker for the diagnosis and the prognosis of colon cancer. PMID: 29806630
    2. MG53 is an effective biomarker of myocardial injury and dysfunction in murine hearts. However, MG53 is not expressed in human heart and therefore does not hold utility as a clinical biomarker of myocardial injury PMID: 26790476
    3. MG53 is a facilitator of rapid injury repair, a mediator of cell migration, and a modulator of myofibroblast differentiation during wound healing PMID: 26306047
    4. Zn(2+) interacts with MG53 in protection against injury to the cell membrane PMID: 25869134
    5. MG53 induces FAK ubiquitination with the aid of UBE2H during skeletal myogenesis. PMID: 24344130
    6. MG53/TRIM72 protein can be directly applied as a therapeutic agent to increase membrane repair capacity of many cell types. PMID: 23699904
    7. data reveal NM-IIA as a key cytoskeleton motor protein that facilitates vesicle trafficking during MG53-mediated cell membrane repair PMID: 22253476
    8. membrane-delimited interaction between MG53 and PTRF contributes to initiation of cell membrane repair PMID: 21343302
    9. MG53, annexin A1, and dysferlin localize to the t-tubule network and show enriched labeling at longitudinal tubules of the t-system in overstretch PMID: 21412170
    10. Crystal structure of PRY-SPRY domain of human TRIM72. PMID: 19967786
    11. Observational study and meta-analysis of gene-disease association. (HuGE Navigator) PMID: 19129174

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  • 亚细胞定位：
    Cell membrane, sarcolemma. Cytoplasmic vesicle membrane.
  • 蛋白家族：
    TRIM/RBCC family
  • 数据库链接：

    HGNC: 32671

    OMIM: 613288

    KEGG: hsa:493829

    STRING: 9606.ENSP00000312675

    UniGene: Hs.661859