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  4. Recombinant Human Tubulin gamma-1 chain (TUBG1)

Recombinant Human Tubulin gamma-1 chain (TUBG1)

  • 货号：
    CSB-YP025334HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP025334HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP025334HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP025334HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP025334HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    TUBG1
  • Uniprot No.：
  • 别名：
    Gamma-1-tubulin; Gamma-tubulin complex component 1; GCP-1; TBG1_HUMAN; TUBG; TUBG1; TUBGCP1; Tubulin gamma-1 chain; Tubulin, gamma 1; Tubulin, gamma polypeptide
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-451
  • 氨基酸序列
    MPREIITLQL GQCGNQIGFE FWKQLCAEHG ISPEGIVEEF ATEGTDRKDV FFYQADDEHY IPRAVLLDLE PRVIHSILNS PYAKLYNPEN IYLSEHGGGA GNNWASGFSQ GEKIHEDIFD IIDREADGSD SLEGFVLCHS IAGGTGSGLG SYLLERLNDR YPKKLVQTYS VFPNQDEMSD VVVQPYNSLL TLKRLTQNAD CVVVLDNTAL NRIATDRLHI QNPSFSQINQ LVSTIMSAST TTLRYPGYMN NDLIGLIASL IPTPRLHFLM TGYTPLTTDQ SVASVRKTTV LDVMRRLLQP KNVMVSTGRD RQTNHCYIAI LNIIQGEVDP TQVHKSLQRI RERKLANFIP WGPASIQVAL SRKSPYLPSA HRVSGLMMAN HTSISSLFER TCRQYDKLRK REAFLEQFRK EDMFKDNFDE MDTSREIVQQ LIDEYHAATR PDYISWGTQE Q
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation.
  • 基因功能参考文献：
    1. CCT actively promotes the formation of conformationally different aggregates of gamma-tubulin, a non-amyloidogenic CCT client protein, which are mediated by specific CCT-gamma-tubulin interactions. PMID: 29339327
    2. that in the face of predominant gamma-tubulin-1 expression, the accumulation of gamma-tubulin-2 in mature neurons and neuroblastoma cells during oxidative stress may denote a prosurvival role of gamma-tubulin-2 in neurons PMID: 28119396
    3. All GCPs are incorporated into the helix via lateral interactions between their N-terminal domains, whereas the C-terminal domains mediate longitudinal interactions with gamma-tubulin. PMID: 27660388
    4. While binding to gamma-tubulin may help target APC to the site of microtubule nucleation complexes, additional C-terminal sequences of APC are required to stimulate and stabilize microtubule growth. PMID: 26556314
    5. Results show that CUL4A- and CUL4B-mediated polyubiquitination of gamma-tubulin for its degradation. PMID: 25542213
    6. In this study, the molecular basis of interaction between two lateral gamma-tubulin units within the gamma-tubulin ring complex were elucidated by making extensive use of molecular mechanics and molecular dynamics techniques. PMID: 25031076
    7. Gamma tubulin expression was associated with patients' age, astrocytoma grade, respectability, patient survival and performance. These factors may be important prognostic indicators for patients with astrocytomas. PMID: 24620973
    8. Recombinant gamma-tubulin can be phosphorylated by Cdk1-cyclin B or Brsk1 and dephosphorylated by Ppp4c-R2-R3A in vitro. PMID: 23966160
    9. Nek9 phosphorylates NEDD1 on Ser377 driving its recruitment and thereby that of gamma-tubulin to the centrosome in mitotic cells. PMID: 22818914
    10. Our results reveal for the first time an increased expression of TUBG1 and TUBG2 in lung cancer PMID: 22806905
    11. overexpression of Cdk1 or BRCA1 greatly expands the gamma-tubulin coating of microtubules, suggesting that the microtubule-bound gamma-tubulin is involved in DNA damage response. PMID: 21951856
    12. in transformed as well as in non-transformed interphase mammalian cells gamma-tubulin is present not only in cytoplasm but also in nuclei and nucleoli where it can be translocated during mitosis PMID: 21465471
    13. These results thus report for the first time the identification of Cep70 as an important centrosomal protein that interacts with gamma-tubulin and underscore its critical role in the regulation of mitotic spindle assembly. PMID: 21795687
    14. The data presented here indicate that gamma-tubulin has a profound relationship with actin filament dynamics PMID: 21473851
    15. a direct interaction with NEDD1 regulates gamma-tubulin recruitment to the centrosome PMID: 20224777
    16. the 2.7-A crystal structure of human gamma-tubulin bound to GTP-gammaS (a non-hydrolysable GTP analogue) PMID: 15917813
    17. analysis of human ninein isoforms that are regulated by centrosomal targeting signals and bind to gamma-tubulin PMID: 17102634
    18. Data suggest that aberrant expression and interactions of betaIII-tubulin and gamma-tubulin may be linked to malignant changes in glial cells. PMID: 17406983
    19. GSK-3beta regulates the localization of gamma-tubulin ring complex, including GCP5, to the spindle poles, thereby controlling the formation of proper mitotic spindles PMID: 18316369
    20. The axial elastic modulus of tubulin diminishes as the length of the monomer increases. PMID: 18621829
    21. Increased expression of centrosomal TUBG in atypical ductal hyperplasia and carcinoma of the breast is reported. PMID: 19215229
    22. Stability of the small gamma-tubulin complex (gamma tubulin/GCP2/GCP3) requires HCA66, a protein of the centrosome and the nucleolus. PMID: 19299467
    23. Data suggest that sequential phosphorylation of Nedd1 by Cdk1 and Plk1 plays a role in targeting gamma tubulin ring complex to the centrosome by promoting the interaction of Nedd1 with the gammaTuRC component gamma-tubulin, during mitosis. PMID: 19509060
    24. Plk1-dependent recruitment of gamma-tubulin complexes to mitotic centrosomes involves multiple PCM components PMID: 19543530
    25. SADB kinase activity controls centrosome homeostasis by regulating phosphorylation of gamma-tubulin. PMID: 19648910

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  • 相关疾病：
    Cortical dysplasia, complex, with other brain malformations 4 (CDCBM4)
  • 亚细胞定位：
    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle.
  • 蛋白家族：
    Tubulin family
  • 数据库链接：

    HGNC: 12417

    OMIM: 191135

    KEGG: hsa:7283

    STRING: 9606.ENSP00000251413

    UniGene: Hs.279669