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  4. Recombinant Human U1 small nuclear ribonucleoprotein 70 kDa (SNRNP70), partial

Recombinant Human U1 small nuclear ribonucleoprotein 70 kDa (SNRNP70), partial

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  • 中文名称：
    人SNRNP70重组蛋白
  • 货号：
    CSB-EP714018HU1a0
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    SNRNP70
  • Uniprot No.：
  • 别名：
    SNRNP70; RNPU1Z; RPU1; SNRP70; U1AP1U1 small nuclear ribonucleoprotein 70 kDa; U1 snRNP 70 kDa; U1-70K; snRNP70
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 来源：
    E.coli
  • 分子量：
    28.7 kDa
  • 表达区域：
    1-210aa
  • 氨基酸序列
    MTQFLPPNLLALFAPRDPIPYLPPLEKLPHEKHHNQPYCGIAPYIREFEDPRDAPPPTRAETREERMERKRREKIERRQQEVETELKMWDPHNDPNAQGDAFKTLFVARVNYDTTESKLRREFEVYGPIKRIHMVYSKRSGKPRGYAFIEYEHERDMHSAYKHADGKKIDGRRVLVDVERGRTVKGWRPRRLGGGLGGTRRGGADVNIRH
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. SNRNP70 binds to the loop I region of U1-snRNA.; Truncated isoforms that lack the RRM domain cannot bind U1-snRNA.; Truncated isoforms that lack the RRM domain cannot bind U1-snRNA.
  • 基因功能参考文献：
    1. These results indicate that the galectin-3-U1 snRNP-pre-mRNA ternary complex is a functional E complex and that U1 snRNP is required to assemble galectin-3 onto an active spliceosome PMID: 27105840
    2. The epitopes of U1-70K autoantibodies have been mapped at single-amino acid resolution. PMID: 26333287
    3. The s propose that Gemin2 is a versatile hub for ribonucleoprotein exchange that functions broadly in RNA metabolism. PMID: 26828962
    4. data lead to a model that FUS functions in coupling transcription to splicing via mediating an interaction between RNAP II and U1 snRNP PMID: 26124092
    5. The selection of 5'-splice site nucleotides by U1 snRNP is achieved predominantly through basepairing with U1 snRNA whilst U1-C fine-tunes relative affinities of mismatched 5'-splice sites. PMID: 25555158
    6. methylation of snRNP70 by SETMAR regulates constitutive and/or alternative splicing PMID: 25795785
    7. U1-70K interacts with the SMN complex and is required for nuclear gem integrity. PMID: 25052091
    8. Data indicate that small nuclear ribonucleoprotein U1-70K in Alzheimer disease (AD) may directly sequester normal soluble forms of U1-70K into insoluble aggregates. PMID: 25355317
    9. [review] The presence of antibodies against U1snRNP autoantigen is considered the serological hallmark of mixed connective tissue disease (MCTD), which is characterized by overlapping features between at least two systemic autoimmune diseases. PMID: 24461387
    10. These studies identify U1 snRNP pathologic changes in brain of early onset genetic forms of Alzheimer's disease PMID: 24773620
    11. inhibition of U1 snRNP also leads to regulation of the usage of In3 pA, suggesting that the C/P activity in the cell can be cross-regulated by splicing, leading to coordination between these two processes. PMID: 23874216
    12. Our results demonstrate unique U1 snRNP pathology and implicate abnormal RNA splicing in Alzheimer's disease pathogenesis. PMID: 24023061
    13. High expression of U1-70K and other U1 small nuclear ribonucleoproteins spliceosome proteins in Alzheimer's disease patients and those with mild cognitive impairment. PMID: 24023061
    14. Serum antibodies against the 70k polypeptides of the U1 ribonucleoprotein complex are associated with psychiatric syndromes in systemic lupus erythematosus. PMID: 22454191
    15. U1-snRNP activates the NOD-like receptor family, pyrin domain-containing 3 (NLRP3) inflammasome in CD14+ human monocytes dependently of anti-U1-snRNP Abs, leading to interleukin (IL)-1beta production. PMID: 22490866
    16. data identify some fungal proteins as possible triggers of anti-U1-70 kDa autoimmunity via molecular mimicry. PMID: 21348812
    17. the hypo-phosphorylated RS domain of SRSF1 interacts with its own RRM, thus competing with U1-70K binding, whereas the hyper-phosphorylated RS domain permits the formation of a ternary complex containing ESE, an SR protein, and U1 snRNP PMID: 21536904
    18. Recruitment of RNAPII, U1-70K and ASF/SF2 protein to transcription sites is splicing independent. PMID: 20519584
    19. crucial association of B19-NS1 in development of autoimmunity by inducing apoptosis and specific cleavage of 70 kDa U1-snRNP PMID: 20500824
    20. U1-70k isoforms control subunit dynamics in the human spliceosomal U1 snRNP PMID: 19784376
    21. Apoptotic U1-70 kd is antigenically distinct from the intact form of the U1-70-kd molecule. PMID: 12115232
    22. A remarkably limited and consistent pattern of T cell targeting of U1-70kDa in connective tissue disease patients is observed in T cell clones generated against U1-70kDa: all clones are specific for epitopes within the RNA binding domain of the protein. PMID: 12218166
    23. Apoptotic modification and the presence of an RNA binding domain may both contribute to autoantigenicity of the lupus U1-70-kDa ribonucleoprotein. PMID: 14688384
    24. Specific binding of heterogenous nuclear ribonucleoprotein E1 (hnRNP E1) and U1 small nuclear ribonucleoprotein (snRNP) in the pre-spliceosomal complex was associated with silencing of pseudoexon splicing. PMID: 17622584
    25. Examine changes in phosphorylation/dephosphorylation balance and the subcellular localization of the U1-70K protein during apoptosis. PMID: 18202700
    26. In a transgenic model of mixed connective tissue disease, T cell recognition of the U1 70-kDa autoantigen by HLA-DR4-transgenic mice is focused on a limited number of T cell epitopes residing primarily within the RNA-binding domain of the RNP autoantigen. PMID: 18523312
    27. Our findings suggest a potential role of CMV in regulation of autoantibodies to snRNPs PMID: 19232124
    28. The novel association of anti-thyroid antibodies with anti-U1RNP antibodies in juvenile onset Systemic Lupus Erythematosus seems to identify a subgroup of patients with less life-threatening organ involvement. PMID: 19419839

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  • 亚细胞定位：
    Nucleus speckle. Nucleus, nucleoplasm.
  • 数据库链接：

    HGNC: 11150

    OMIM: 180740

    KEGG: hsa:6625

    STRING: 9606.ENSP00000472998

    UniGene: Hs.467097