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  4. Recombinant Human Ubiquilin-1 (UBQLN1)

Recombinant Human Ubiquilin-1 (UBQLN1)

  • 货号：
    CSB-YP892477HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP892477HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP892477HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP892477HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    DA41; DSK2; FLJ90054; hPLIC-1; hPLIC1; PLIC-1; PLIC1; Protein linking IAP with cytoskeleton 1; Ubiquilin-1; Ubiquilin1; UBQL1_HUMAN; UBQLN1; UBQN; XDRP1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-589
  • 氨基酸序列
    AESGESGGP PGSQDSAAGA EGAGAPAAAA SAEPKIMKVT VKTPKEKEEF AVPENSSVQQ FKEEISKRFK SHTDQLVLIF AGKILKDQDT LSQHGIHDGL TVHLVIKTQN RPQDHSAQQT NTAGSNVTTS STPNSNSTSG SATSNPFGLG GLGGLAGLSS LGLNTTNFSE LQSQMQRQLL SNPEMMVQIM ENPFVQSMLS NPDLMRQLIM ANPQMQQLIQ RNPEISHMLN NPDIMRQTLE LARNPAMMQE MMRNQDRALS NLESIPGGYN ALRRMYTDIQ EPMLSAAQEQ FGGNPFASLV SNTSSGEGSQ PSRTENRDPL PNPWAPQTSQ SSSASSGTAS TVGGTTGSTA SGTSGQSTTA PNLVPGVGAS MFNTPGMQSL LQQITENPQL MQNMLSAPYM RSMMQSLSQN PDLAAQMMLN NPLFAGNPQL QEQMRQQLPT FLQQMQNPDT LSAMSNPRAM QALLQIQQGL QTLATEAPGL IPGFTPGLGA LGSTGGSSGT NGSNATPSEN TSPTAGTTEP GHQQFIQQML QALAGVNPQL QNPEVRFQQQ LEQLSAMGFL NREANLQALI ATGGDINAAI ERLLGSQPS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays an important role in the regulation of different protein degradation mechanisms and pathways including ubiquitin-proteasome system (UPS), autophagy and endoplasmic reticulum-associated protein degradation (ERAD) pathway. Mediates the proteasomal targeting of misfolded or accumulated proteins for degradation by binding (via UBA domain) to their polyubiquitin chains and by interacting (via ubiquitin-like domain) with the subunits of the proteasome. Plays a role in the ERAD pathway via its interaction with ER-localized proteins UBXN4, VCP and HERPUD1 and may form a link between the polyubiquitinated ERAD substrates and the proteasome. Isoform 1, isoform 2 and isoform 3 play a role in unfolded protein response (UPR) by attenuating the induction of UPR-inducible genes, DDTI3/CHOP, HSPA5 and PDIA2 during ER stress. Involved in the regulation of macroautophagy and autophagosome formation; required for maturation of autophagy-related protein LC3 from the cytosolic form LC3-I to the membrane-bound form LC3-II and may assist in the maturation of autophagosomes to autolysosomes by mediating autophagosome-lysosome fusion. Negatively regulates the TICAM1/TRIF-dependent toll-like receptor signaling pathway by decreasing the abundance of TICAM1 via the autophagic pathway. Isoform 1 and isoform 3 play a key role in the regulation of the levels of PSEN1 by targeting its accumulation to aggresomes which may then be removed from cells by autophagocytosis. Promotes the ubiquitination and lysosomal degradation of ORAI1, consequently downregulating the ORAI1-mediated Ca2+ mobilization. Suppresses the maturation and proteasomal degradation of amyloid beta A4 protein (A4) by stimulating the lysine 63 (K63)-linked polyubiquitination. Delays the maturation of A4 by sequestering it in the Golgi apparatus and preventing its transport to the cell surface for subsequent processing.
  • 基因功能参考文献：
    1. Thus, UBQLN1 plays an important role in clearing mislocalized mitochondrial proteins upon cell stimulation, and its absence leads to suppression of protein synthesis and cell cycle arrest. PMID: 28933694
    2. UBQLN1 variant was not associated with the risk of Alzheimer's disease. PMID: 28719358
    3. The STI and UBA Domains of UBQLN1 Are Critical Determinants of Substrate Interaction and Proteostasis. PMID: 28075048
    4. Following the loss of UBQLN1 in lung adenocarcinoma cells, there is an accelerated loss of IGF1R. PMID: 29054976
    5. The evidence of genetic associations has been found for the multivariate response phenotype that involves trans effects modulating expression of genes following heat shock, including HSF1 and UBQLN1. PMID: 27553423
    6. The expression level of UBQLN1 and prognosis in breast cancer is clarified for the first time and UBQLN1 seems to be a novel molecular marker to predict poor prognosis in breast cancer. PMID: 26406952
    7. The results suggest that UBQ-8i polymorphism may contribute to Alzheimer's disease susceptibility, but does not synergize with APOEepsilon4 status to increase Alzheimer's disease risk. PMID: 25010605
    8. These results suggest that the UBQ-8i polymorphism is associated with Alzheimer's disease risk. PMID: 25387430
    9. found that ZEB1 is required for induction of mesenchymal-like properties following loss of UBQLN1 and ZEB1 is capable of repressing expression of UBQLN1 PMID: 24747970
    10. High UBQLN1 expression is associated with low radiosensitivity in breast cancer. PMID: 25044403
    11. Ubiquilin-1 immunoreactivity is concentrated on Hirano bodies and dystrophic neurites in Alzheimer's disease PMID: 23421764
    12. Human ubiquilin-1 overexpression in transgenic mice increases the lifespan and delays accumulation of Huntingtin aggregates in the R6/2 mouse model of Huntington's disease. PMID: 24475300
    13. The results demonstrate that in Alzheimer's disease hippocampus, ubiquilin-1 immunoreactivity increases in the neuronal nucleoplasm and is associated with region-specific neurofibrillary changes. PMID: 23869942
    14. Targeting of Ubqln1 to autophagosomes requires the Ubqln4 UBL domain and the Ubqln1 UBA domain. PMID: 23459205
    15. data suggest that ubiquilin-1 modulates gamma-secretase-mediated epsilon-site cleavage and thus may play a role in regulating gamma-secretase cleavage of various substrates. PMID: 23663107
    16. Genetic variants in UBQLN1 are not commonly associated with amyotrophic lateral sclerosis PMID: 22766032
    17. This study demonistrated that Allele C of polymorphism UBQ-8i of the UBQLN1 gene is not an independent risk factor for mild cognitive impairment or Alzheimer's disease PMID: 22272618
    18. Ubiquilin-1 was over-expressed following antiproliferative agents treatment of ovarian cancer cells. PMID: 22134777
    19. ubiquilin-1 chaperone activity is necessary to regulate the production of APP and its fragments and that diminished ubiquilin-1 levels may contribute to AD pathogenesis. PMID: 21852239
    20. PLIC-1 is a novel inhibitor of the TLR3-Trif antiviral pathway by reducing the abundance of Trif PMID: 21695056
    21. specific ubiquilin-1 transcript variants can cause PS1 accumulation and aggresome formation PMID: 21143716
    22. Data show that ubiquilin is degraded during both macroautophagy and during chaperone-mediated autophagy (CMA). PMID: 20529957
    23. The UBQ-8i polymorphism of the UBQLN1 gene is extremely rare in Taiwan Chinese and unlikely to play a significant role in the risk of AD in Taiwan Chinese. PMID: 20350585
    24. PLIC1 may be a regulator of HCV RNA replication through interaction with NS5B. Nn Huh7 cells that express an HCV subgenomic replicon, the amounts of both NS5B and the replicon RNA were reduced by overexpression of PLIC1. PMID: 12634373
    25. Ubiquilin proteins play an important role in regulating PS protein levels in cells. PMID: 15004330
    26. Ubiquilin-1 plays an active role in the precise regulation of HASH-1 and of other tissue-specific bHLH proteins PMID: 15492808
    27. Our findings suggest that genetic variants in UBQLN1 on chromosome 9q22 substantially increase the risk of Alzheimer's disease, possibly by influencing alternative splicing of this gene in the brain PMID: 15745979
    28. ubiquilin-1 limits the availability of unassembled nicotinic acetylcholine receptor subunits in neurons by drawing them to the proteosome, thus regulating nicotine-induced up-regulation PMID: 16091357
    29. Genetic variation in the UBQLN1 gene has a modest effect on risk, age of onset and disease duration of Alaheimer's disease. PMID: 16302009
    30. Overexpression of ubiquilin reduces cell death in HeLa cells and primary neurons stably expressing green fluorescent protein-huntingtin fusion protein. PMID: 16461334
    31. Results suggest that UBQLN1 variants do not increase risk for Alzheimer disease. PMID: 16526030
    32. Data show that ubiquilin 1 interacts both with presenilin 1 (PS1) holoprotein and heterodimer and that the interaction between PS1 and ubiquilin 1 takes place near the cell surface. PMID: 16815845
    33. UBQLN1 modulates amyloid precursor protein trafficking and Abeta secretion. PMID: 16945923
    34. These results indicate a role for PLIC-1 in the protein aggregation-stress pathway, and we propose a novel function for the ubiquitin-like (UBL) domain--by means of UBL-UIM interactions--in transport to aggresomes. PMID: 17082820
    35. Mutation of two lysine residues in the PS2-loop region suggested that ubiquitination is not required for interaction with ubiquilin-1 and may, in fact, even negatively regulate the interaction. PMID: 17614368
    36. Our results suggest that it is unlikely that the SNP rs12344615 of the UBQLN1 gene is related to the onset of AD, PD or cognitive function. PMID: 17709205
    37. Expression of the human Alzheimer's disease-associated variant of UBQLN1 leads to more severe degeneration than does comparable expression of the human wildtype UBQLN1 in Drosophila eye. PMID: 17947293
    38. The three-dimensional structure of the UBA domain of ubiquilin-1 (UQ1-UBA) free in solution and in complex with ubiquitin, is described. PMID: 18241885
    39. Risk estimation for AA versus GG genotypes showed that the AA genotype is a weak risk factor for Alzheimer's disease PMID: 18340109
    40. Plic-1 may play a significant role in regulating the strength of synaptic inhibition by increasing the stability of GABA(A)Rs within the secretory pathway and thereby promoting their insertion into the neuronal plasma membrane. PMID: 18467327
    41. overexpression UBQLN1 transcript variants TV1-3, but not TV4, exert a protective effect during the unfolded protein response by attenuating CHOP induction and potentially increasing cell viability. PMID: 18953672
    42. siRNA-mediated UBQLN1 depletion made cells more susceptible to starvation-induced cell death. UBQLN1 regulates cell survival during starvation. PMID: 19148225

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Endoplasmic reticulum. Cytoplasmic vesicle, autophagosome. Cell membrane.
  • 组织特异性：
    Brain (at protein level). Ubiquitous. Highly expressed throughout the brain; detected in neurons and in neuropathological lesions, such as neurofibrillary tangles and Lewy bodies. Highly expressed in heart, placenta, pancreas, lung, liver, skeletal muscle
  • 数据库链接：

    HGNC: 12508

    OMIM: 605046

    KEGG: hsa:29979

    STRING: 9606.ENSP00000365576

    UniGene: Hs.9589