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  4. Recombinant Human Ubiquitin-conjugating enzyme E2 C (UBE2C)

Recombinant Human Ubiquitin-conjugating enzyme E2 C (UBE2C)

  • 货号：
    CSB-YP025440HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP025440HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP025440HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP025440HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP025440HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Cyclin selective ubiquitin carrier protein; dJ447F3.2; Mitotic specific ubiquitin conjugating enzyme; UB E2C; UBCH 10; UbcH10; UBE 2C; Ube2c; UBE2C_HUMAN; Ubiquitin carrier protein C; Ubiquitin carrier protein E2 C; Ubiquitin carrier protein E2C; Ubiquitin conjugating enzyme E2 C; Ubiquitin conjugating enzyme E2C; Ubiquitin protein ligase C; Ubiquitin-conjugating enzyme E2 C; Ubiquitin-protein ligase C
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-179
  • 氨基酸序列
    ASQNRDPAA TSVAAARKGA EPSGGAARGP VGKRLQQELM TLMMSGDKGI SAFPESDNLF KWVGTIHGAA GTVYEDLRYK LSLEFPSGYP YNAPTVKFLT PCYHPNVDTQ GNICLDILKE KWSALYDVRT ILLSIQSLLG EPNIDSPLNT HAAELWKNPT AFKKYLQETY SKQVTSQEP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.
  • 基因功能参考文献：
    1. UBE2C is involved in the anti-proliferative and pro-apoptotic functions of ECRG4 in esophageal squamous cell carcinoma. PMID: 29268240
    2. loss of BRCA1 function results in an increase in UBE2C expression and chemical resistance to doxorubicin in breast cancer cells. PMID: 28881292
    3. FoxM1 promotes glioma progression by enhancing UBE2C transcription PMID: 28767320
    4. SAG/RBX2 E3 ligase complexes with UBCH10 and UBE2S ubiquitin-conjugating enzymes to ubiquitylate beta-TrCP1 via K11-linkage for degradation. PMID: 27910872
    5. UBE2C and HOXA1 RNA and protein are differentially expressed in conventional and Spitz nevi and melanoma. PMID: 28657121
    6. High UBE2C expression is associated with esophageal squamous cell carcinoma. PMID: 27588470
    7. Urinary UBE2C cell-free RNA may be a valuable diagnostic marker for bladder cancer. PMID: 27528424
    8. A role was identified for UBE2C as a marker of the androgen signaling pathway in prostate cancer. PMID: 27550197
    9. Data suggest that the activity of AURKA might be regulated by UBE2C through regulating the activity of anaphasepromoting complex. UBE2C may be a new marker in the diagnosis of gastric cancer and may be a potential therapeutic target for the treatment of gastric adenocarcinoma. PMID: 28260026
    10. High UBCH10 expression is associated with bortezomib-resistance in multiple myeloma. PMID: 28000886
    11. the expression of UbcH10 in colorectal cancer samples as compared with healthy tissue sample from the same patient according to age at surgery, was examined. PMID: 26744065
    12. UbcH10 may promote gastric cancer growth. PMID: 27349176
    13. Identification of UBE2C as a target of wild-type and GOF mutant p53 further highlights the contribution of p53 in regulation of spindle assembly checkpoint PMID: 27129209
    14. The anaphase-promoting complex/cyclosome C activity in human cells is tuned by the combinatorial use of three E2 ubiquitin-conjugating enzymes, namely UBE2C, UBE2S, and UBE2D. PMID: 26904940
    15. Study shows an overexpression of UbcH10 mRNA and protein in the vast majority of colorectal cancer (CRC) patients analyzed and indicates that UbcH10 expression regulates CRC growth. PMID: 25917796
    16. the present study showed that miR-196a promoted cell proliferation by targeting UBE2C in breast cancer. Thus, miR-196a may be a potential oncogene in breast cancer and a promising therapeutic target in breast cancer treatment. PMID: 26062455
    17. UBE2C could regulate phospho-ERK1/2 level. PMID: 25832867
    18. Data suggest that ubiquitin-conjugating enzyme UbcH10 may be a promising target for the therapy of breast cancer. PMID: 25739083
    19. Upregulation of miR-17/20a promotes gastric cancer cell growth by targeting UBE2C. PMID: 25760688
    20. UbcH10 overexpression increases carcinogenesis and blocks ALLN susceptibility in colorectal cancer. PMID: 25376843
    21. in addition to binding APC11's RING, UBCH10 is corecruited via interactions with APC2 PMID: 25825779
    22. excess E2F1 due to Rb inactivation recruits the complex of Cdc20 and the anaphase-promoting complex/cyclosome to deregulate the expression of UBCH10 PMID: 25368385
    23. UBE2C may be a marker for diagnosis of nonpalpable breast lesions but not benign or malignant tumors in mammography core biopsies. Suppression of UBE2C may be a potential therapy target in breast cancer. PMID: 24699941
    24. the UBE2C gene is capable of promoting proliferation and invasion of lung cancer cells. Therefore, UBE2C may play a pivotal role in development and invasion of lung cancer. PMID: 24815438
    25. Results suggest that UBE2C acts as an oncogene in prostate cancer progression. PMID: 24512726
    26. Data suggest that ubiquitin conjugating enzyme UBE2C may serve as a potential therapeutic target aimed at inducing radiation and chemo sensitization. PMID: 24072565
    27. Elevated levels of S100A8 and UBE2C were observed. PMID: 24114735
    28. UBE2C positivity is independently associated with shorter cancer-specific survival in bladder cancer patients who underwent radical cystectomy. PMID: 23826418
    29. Data indicate that expression-based risk indices of three genes UBE2C, TPX2, and MELK were more strongly associated with poor 5-year survival in adenocarcinoma patients. PMID: 23357462
    30. High expression of UbcH10 was associated with pancreatic ductal adenocarcinoma. PMID: 23355337
    31. the expression of cyclin D1, MCM7, TRIM29, and UBE2C was found to be significantly associated with progression to muscle-invasive bladder cancer. PMID: 23201130
    32. UbcH10 overexpression has a critical role in lung carcinogenesis, and the evaluation of UbcH10 expression levels may be a new tool for the characterisation of non small cell lung carcinoma. PMID: 23102841
    33. Data sugggest that UbcH10 may play an important role in non-small cell lung cancer (NSCLC) carcinogenesis, and silencing UbcH10 may represent a potential therapeutic strategy for NSCLC. PMID: 22760214
    34. Overexpression of UbcH10 may be a useful indicator of endometrial carcinoma. PMID: 22415060
    35. growth rate of SiHa and HeLa transfected with dominant negative-UBE2C was significantly reduced compared to vector control PMID: 22694363
    36. High UBE2C mRNA expression was found to be of adverse prognostic significance in high-risk breast cancer patients PMID: 22056852
    37. UbcH10 expression was associated with tumor invasion of the portal vein, tumor size, TNM staging, and tumor differentiation in hepatocellular carcinoma. PMID: 21354912
    38. Effect of UBE2C overexpression on the proliferation of 293T cell line PMID: 21651863
    39. Mediator complex subunit (MED)1 phosphorylation leads to UBE2C locus looping, UBE2C gene expression and cell growth in castration-resistant prostate cancer. PMID: 21556051
    40. Spindle assembly checkpoint protein Cdc20 transcriptionally activates expression of ubiquitin carrier protein UbcH10. PMID: 21454660
    41. Elevated expression of UbcH10 is associated with breast cancer. PMID: 19038004
    42. elevated levels of Ubch10 and Cdc20 degrade cyclin B in hpv-16 iinfected cells, required for exit from mitosis, permitting initiation of the next round of DNA synthesis and cell cycle progression. PMID: 20739533
    43. The unstructured N-terminal region of UbcH10 directly binds the 19 S regulatory complex of the 26 S proteasome, and it mediates the initiation of substrate translocation. PMID: 20007692
    44. The overexpression of UbcH10 gene plays a critical role in the carcinogenesis and tumor progression of colorectal cancer. PMID: 19779934
    45. show that UbcH10 overexpression leads to precocious degradation of cyclin B by the anaphase-promoting complex/cyclosome, supernumerary centrioles, lagging chromosomes, and aneuploidy. PMID: 20065091
    46. Data imply that knocking-down UbcH10 protein expression may represent a potential therapeutic option for glioma. PMID: 19657671
    47. UbcH10 is highly expressed in various human primary tumors and UbcH10 has an ability to promote cell growth and malignant transformation. PMID: 12874022
    48. UbcH10 plays an important role in tumor development and that its inhibition in combination with agonists of the TRAIL receptor may provide an enhanced therapeutic index PMID: 15208666
    49. UbcH10 overexpression is involved in thyroid cell proliferation and may represent a marker of thyroid anaplastic carcinomas PMID: 16106252
    50. results showing aberrations in levels of gene expression and locus copy number of ubiquitin-conjugating enzyme E2C gene (UBE2C) suggest that this gene may play an important role in tumor progression leading to advanced colon cancer with liver metastasis PMID: 16772118

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  • 蛋白家族：
    Ubiquitin-conjugating enzyme family
  • 数据库链接：

    HGNC: 15937

    OMIM: 605574

    KEGG: hsa:11065

    STRING: 9606.ENSP00000348838

    UniGene: Hs.93002