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  4. Recombinant Human Ubiquitin-conjugating enzyme E2 D1 (UBE2D1)

Recombinant Human Ubiquitin-conjugating enzyme E2 D1 (UBE2D1)

  • 中文名称：
    人UBE2D1重组蛋白
  • 货号：
    CSB-YP025443HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人UBE2D1重组蛋白
  • 货号：
    CSB-EP025443HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人UBE2D1重组蛋白
  • 货号：
    CSB-EP025443HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人UBE2D1重组蛋白
  • 货号：
    CSB-BP025443HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人UBE2D1重组蛋白
  • 货号：
    CSB-MP025443HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    E2(17)KB1; SFT; Stimulator of Fe transport; UB2D1_HUMAN; UBC 4/5; UBC4/5; UBC4/5 homolog; UBC4/5, S. cerevisiae, homolog of; UBCH 5; UBCH 5A; UbcH5; UBCH5A; Ube2d1; Ubiquitin carrier protein; Ubiquitin carrier protein D1; Ubiquitin conjugating enzyme E2 17 kDa 1; Ubiquitin conjugating enzyme E2 D1; Ubiquitin conjugating enzyme E2D 1 (UBC4/5 homolog yeast); Ubiquitin conjugating enzyme E2D 1; Ubiquitin conjugating enzyme UBCH5A; Ubiquitin protein ligase D1; Ubiquitin-conjugating enzyme E2 D1; Ubiquitin-conjugating enzyme E2(17)KB 1; Ubiquitin-conjugating enzyme E2-17 kDa 1; Ubiquitin-protein ligase D1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-147
  • 氨基酸序列
    MALKRIQKEL SDLQRDPPAH CSAGPVGDDL FHWQATIMGP PDSAYQGGVF FLTVHFPTDY PFKPPKIAFT TKIYHPNINS NGSICLDILR SQWSPALTVS KVLLSICSLL CDPNPDDPLV PDIAQIYKSD KEKYNRHARE WTQKYAM
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of STUB1, TRAF6 and TRIM63/MURF1. Ubiquitinates STUB1-associated HSP90AB1 in vitro. Lacks inherent specificity for any particular lysine residue of ubiquitin. Essential for viral activation of IRF3. Mediates polyubiquitination of CYP3A4.
  • 基因功能参考文献：
    1. The anaphase-promoting complex/cyclosome C activity in human cells is tuned by the combinatorial use of three E2 ubiquitin-conjugating enzymes, namely UBE2C, UBE2S, and UBE2D. PMID: 26904940
    2. Data show that ubiquitin E2 enzymes UBE2D1/2/3 and E3 ligase RNF138 accumulate at DNA-damage sites and act at early resection stages by promoting CtIP protein ubiquitylation and accrual. PMID: 26502057
    3. The Asp17 mutation in MDM2 stimulated its discharge of the UBCH5a-ubiquitin thioester adduct. PMID: 25543083
    4. The SMURF2:UBCH5 complex is critical in maintaining KRAS protein stability. PMID: 24709419
    5. Biochemical characterization of recombinant UBTD1 and UBE2D demonstrated that the two proteins form a stable, stoichiometric complex that can be purified to near homogeneity. PMID: 24211586
    6. crystal structure of the dimeric RING domain of rat RNF4 in complex with E2 (UbcH5A) linked by an isopeptide bond to ubiquitin PMID: 22842904
    7. These data demonstrate that the ability of ICP0 to interact with cellular E2 ubiquitin-conjugating enzyme UBE2D1 is fundamentally important for its biological functions during HSV-1 infection. PMID: 22438555
    8. identify the IDOL-UBE2D complex as an important determinant of LDLR activity, and provide insight into molecular mechanisms underlying the regulation of cholesterol uptake PMID: 21685362
    9. This structure reveals an interaction between the ubiquitin surface flanking K11 and residues adjacent to the E2 catalytic cysteine and suggests a possible role for this surface in formation of K11 linkages. PMID: 21396940
    10. The s demonstrate that c-IAP1 and UbcH5 family promote K11-linked polyubiquitination of receptor-interacting protein 1 (RIP1) in vitro and in vivo. PMID: 21113135
    11. Whereas UbcH5(A, B and C) is highly efficient in converting IkBa into monoubiquitinated forms, Cdc34 drives ubiquitin (Ub)-Ub conjugation PMID: 20347421
    12. conformational changes in CHIP upon binding of Hsp70, Hsp90, and their respective C-terminal EEVD peptides, and in complex with the different E2 ubiquitin-conjugating enzymes UbcH5a and Ubc13 PMID: 20146531
    13. UbcH5A is up-regulated in the liver of iron-overloaded patients with hereditary hemochromatosis, but in vitro studies failed to show regulation in response to iron loading or chelation. Earlier in vivo mRNA expression data for SFT might be UbcH5A. PMID: 12480712
    14. comparison of the regional distribution of SFT/UbcH5A and DMT1 mRNA in the adult brain PMID: 15139022
    15. Ro52 has both cytoplasmic and nuclear substrates, and mediates ubiquitination through UBE2D1 in the cytoplasm and through UBE2E1 in the nucleus. PMID: 18845142
    16. Catalytically active Ubc5 is required for IRF3 activation by viral infection. PMID: 19854139

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Ubiquitin-conjugating enzyme family
  • 组织特异性：
    Ubiquitous. Up-regulated in livers of iron-overloaded patients with hereditary hemochromatosis.
  • 数据库链接：

    HGNC: 12474

    OMIM: 602961

    KEGG: hsa:7321

    STRING: 9606.ENSP00000363019

    UniGene: Hs.129683