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  4. Recombinant Human Vitamin D-binding protein (GC), partial

Recombinant Human Vitamin D-binding protein (GC), partial

In Stock
  • 货号：
    CSB-YP009306HU
  • 规格：
    ￥1500
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP009306HU could indicate that this peptide derived from Yeast-expressed Homo sapiens (Human) GC.
    • Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP009306HU could indicate that this peptide derived from Yeast-expressed Homo sapiens (Human) GC.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    GC
  • Uniprot No.：
  • 别名：
    DBP; DBP/GC; GC; Gc globulin; Gc-globulin; GRD3; Group specific component; Group specific component vitamin D binding protein; Group-specific component; hDBP; VDB; VDBG; VDBP; Vitamin D binding alpha globulin; Vitamin D-binding protein; VTDB_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 来源：
    Yeast
  • 分子量：
    67.0kDa
  • 表达区域：
    19-474aa
  • 氨基酸序列
    RGRDYEKNKVCKEFSHLGKEDFTSLSLVLYSRKFPSGTFEQVSQLVKEVVSLTEACCAEGADPDCYDTRTSALSAKSCESNSPFPVHPGTAECCTKEGLERKLCMAALKHQPQEFPTYVEPTNDEICEAFRKDPKEYANQFMWEYSTNYGQAPLSLLVSYTKSYLSMVGSCCTSASPTVCFLKERLQLKHLSLLTTLSNRVCSQYAAYGEKKSRLSNLIKLAQKVPTADLEDVLPLAEDITNILSKCCESASEDCMAKELPEHTVKLCDNLSTKNSKFEDCCQEKTAMDVFVCTYFMPAAQLPELPDVELPTNKDVCDPGNTKVMDKYTFELSRRTHLPEVFLSKVLEPTLKSLGECCDVEDSTTCFNAKGPLLKKELSSFIDKGQELCADYSENTFTEYKKKLAERLKAKLPDATPTELAKLVNKHSDFASNCCSINSPPLYCDSEIDAELKNIL
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMOSTAR-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The process of expressing the recombinant human GC protein in the yeast requires the recombinant DNA gene formed by the integration of encoding gene for the 19-474aa of the human GC protein and N-terminal 6xHis-SUMOSTAR tag sequence, the expression vector that the recombinant DNA gene inserts into, the yeast that provided the necessary macromolecules and components for transcription and translation of the cloned expression vector. After isolation and purification, this N-terminal 6xHis-SUMOSTAR-tagged recombinant GC protein was obtained. This recombinant GC protein is characterized by high purity (>90%, SDS-PAGE). This GC protein ran along the gel to the band of approximately 52 kDa molecular weight.

    GC is a gene providing instruction of making a protein named Vitamin D-binding protein (also abbreviated as VDB or DBP) in human and belongs to ALB/AFP/VDB family. DBP is a multifunctional protein that is well-conserved in the evolution of vertebrates. This protein plays a crtical role in vitamin D  metabolites. In addtion to transporting vitamin D metabolites, DBP is proposed to be involved in the transport of fatty acids, in complement C5a-mediated chemotaxis, and in the activation of macrophages.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

Could you quote the following product?
1. Is 10ug the minimum quantity?
2. What is the shelf life of both liquid form and powder form?

A:
Very nice to receive your inquiry. Recombinant Human Vitamin D-binding protein(GC),partial
CSB-YP009306HU 15-20 working days
CSB-EP009306HU 3-5 working days
Expression Region: 19-474aa, fragment
Tag Info: YP: N-terminal 6xHis-tag; EP: N-terminal 6xHis-tag
Target Protein Sequence:

RGRDYEKNKVCKEFSHLGKEDFTSLSLVLYSRKFPSGTFEQVSQLVKEVVSLTEACCAEGADPDCYDTRTSALSAKSCESNSPFPVHPGTAECCTKEGLERKLCMAALKHQPQEFPTYVEPTNDEICEAFRKDPKEYANQFMWEYSTNYGQAPLSLLVSYTKSYLSMVGSCCTSASPTVCFLKERLQLKHLSLLTTLSNRVCSQYAAYGEKKSRLSNLIKLAQKVPTADLEDVLPLAEDITNILSKCCESASEDCMAKELPEHTVKLCDNLSTKNSKFEDCCQEKTAMDVFVCTYFMPAAQLPELPDVELPTNKDVCDPGNTKVMDKYTFELSRRTHLPEVFLSKVLEPTLKSLGECCDVEDSTTCFNAKGPLLKKELSSFIDKGQELCADYSENTFTEYKKKLAERLKAKLPDATPKELAKLVNKRSDFASNCCSINSPPLYCDSEIDAELKNIL


1) CSB-YP009306HU is a customized protein, and the smallest size is 500ug. CSB-EP009306HU is a stock protein, and the smallest size is 10ug.
2) The shelf life of liquid-form protein will be 6 months around at -20℃/-80℃. The shelf life of lyophilized form is 12 months around at -20℃/-80℃.
Q:

I am interested in the Recombinant Human Vitamin D-binding protein(GC) product that you manufacture and would very much appreciate if you would answer all my questions.
1. First and most importantly, is this protein registered with the FDA as a food source, or a pharmaceutical?
2. Are there any license/credentials required to be able to obtain this product from you?
3. Could you tell me how other companies are using the protein?
4. What are your recommended uses for this ingredient?
5. Are there any ingredients that if you were to add your protein to it, that would render the protein unsafe, or cause the protein to lose its potency?
6.What would be the best stabilizing ingredients to go along with the protein?
7. Which of the two kinds that you sell would perform the best and have the longest shelf-life?
8. when/why would you choose the liquid over the freeze-dried version?
9. Are there any restrictions adding it to a cream base that you know of?
10. Any applications you don't advise using it in or for?
11. What are the best fats to combine with the protein for it to be the most effective it can be?
12. When you mention "buffer" I'm assuming you're talking about buffering the protein with other ingredients, in a cream/gel etc.?
13. What is the cost of the protein and in what sizes do you sell them?
14. what is the recommended usage rate of both your products?

A:
Very nice to receive your inquiry. The reply is as follows:
1. Our products are only used for scientific research and have not passed FDA certification.
2. This product can be purchased directly without a license/certificate.
3. This protein is only used for scientific research and is not used for other purposes.
As for the application of this protein, we did not test it. The literature we found is all about the research on the structure and activity-related factors of this protein, providing references:
http://www.uniprot.org/uniprot/P02774/publications
https://www.ncbi.nlm.nih.gov/pubmed/? Term = Vitamin % 20 d - binding protein % 20
4. This protein has been analyzed for its immunogenicity, which is good for immunogenicity experiments.
5. We the default buffer is Tris - -based buffer, 50% glycerol, we usually according to the actual circumstances of the protein, to provide the most suitable save buffer to prevent protein instability or failure will not add other ingredients.
6. The form of lyophilized powder theoretically causes the protein to lose water without affecting other functions, but we have not verified it.
7. The shelf life of freeze-dried powder is one year, and the shelf life of liquid state is half a year. When the customer asks for freeze-dried powder or we find that the stability of the protein is not very good, we will advise the customer to choose the form of freeze-dried powder for fear of the loss of protein in the transportation process.
In general, if there is no special case, we will advise the customer to use liquid form.
Q:

We are hoping to get the same lot as a previous order you sent to us: Lot# 03103. Do you know if there has been any change between the expression processes back in 2016 for Lot# 03103, and the current lot that is being expressed?
We are interested in purchasing 2mg of the Yeast-expressed protein, with Endotoxin Removal + Aseptic Processing. Would it be possible to get 2mg of the same protein as lot# 03103, with endotoxin removal and aseptic processing? If so, can you please provide pricing for this quantity?

A:
Thanks for your inquiry. You previously requested for Tag removal, but we were failed to remove the tag, so it was finally shipped with N-terminal 6xHis-tagged; Liquid form; 500ug; Concentration: 0.3mg/ml; Volume: 1.7ml; Buffer: 0.2 μm Sterile Filtered 10mM Tris-HCl, 1 mM EDTA, pH 8.0, 50% glycerol; Requirements: Endotoxin Removal and Aseptic processing
We can provide the Datasheet and COA of the previous Lot # 03103 for your reference.
We can meet your demand to provide 2mg of CSB-YP009306HU and keep it the same as Lot # 03103. There is no change between the current and the previous expression processes.
We will keep the same expression process and raw material to maintain batch-to-batch consistency.
Recombinant Human Vitamin D-binding protein(GC), partial
CSB-YP009306HU
Expression Region: 19-474aa; Partial of NM_000583.3
Tag: N-terminal 6xHis-tagged
Requirements:
Liquid form, keep it the same as Lot # 03103.
Buffer: 0.2 μm Sterile Filtered 10mM Tris-HCl, 1 mM EDTA, pH 8.0, 50% glycerol
Endotoxin Removal and Aseptic processing
Q:

I ordered this protein last time and I'm very happy with the protein.
I'd like to check if you have this protein in stock now ? I should be able to send an order to you very soon.

A:
Thanks for your inquiry.
CSB-YP009306HU Recombinant Human Vitamin D-binding protein(GC), partial
Expression Region: 19-474aa; Partial.
Tag information: N-terminal 6xHis-sumostar-tagged;
Lead time: 3-7 working days (In Stock)
We checked that you ordered this protein on 2018-0125, Batch: 03683. We still have about 1mg of the same batch protein in stock, but now the protein in stock is in lyophilized, which will be better for long time storage.If you want to order this protein, pls let us know if you need the liquid form or lyophilized form, we can provide the same batch protein both in liquid or lyophilized form.

靶点详情

  • 功能：
    Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation.
  • 基因功能参考文献：
    1. VDBP in the cervicovaginal fluid (CVF) independently predicts intra-amniotic infection and imminent preterm delivery in women with PTL, whereas in women with preterm premature rupture of membranes, an elevated VDBP level in CVF is not associated with increased risks of these two outcome variables. PMID: 29879190
    2. SNPs of the VDR and GC genes are associated with vitamin D deficiency in postmenopausal Mexican women. PMID: 30150596
    3. GC rs7041 genotype modified the effects of pregnancy on maternal and placental vitamin D metabolism. PMID: 29196501
    4. Urinary VDBP correlated with proteinuria and renal SLE disease activity index, and predicted the development of proteinuria in lupus nephritis. PMID: 29958502
    5. Findings implied that VDBP rs7041-G and rs3733359-T variants may contribute to increased susceptibility to HCV infection in a high-risk Chinese population. PMID: 30218750
    6. Polymorphisms of VDBP rs4588 and rs2282679 may play a potentially important role in epilepsy susceptibility. PMID: 29993274
    7. The study findings suggested a possible clinical application of uVDPB as an early and a good marker for the detection of early renal disease in type 2 diabetes mellitus Saudi patients. PMID: 29850609
    8. GC gene variant has no effect on 25-hydroxyvitamin D levels. PMID: 28892641
    9. genetic association study in population in north India: Data suggest (1) GT allele of VDBP SNP rs7041, (2) VDBP allelic combination (GC1F/1F: T allele rs4588; C allele rs7041), and (3) GA allele of CYP2R1 SNP rs2060793 are associated with vitamin D deficiency in women with PCOS (polycystic ovarian syndrome) in population studied. (VDBP = vitamin D-binding protein; CYP2R1 = cytochrome P450 family 2 subfamily R member 1) PMID: 28008453
    10. The A allele of VDBP gene polymorphism might be a potential risk factor for progression of chronic urticarial. PMID: 29165650
    11. The present study indicates an association between VDR and vitamin D binding protein Single Nucleotide Polymorphisms and Type 1 Diabetes Mellitus among Turkish subjects. PMID: 29506625
    12. Survival analyses showed that the vitamin D binding protein C allele was correlated with poor disease-free survival (DFS). PMID: 29409465
    13. These findings showed that racial/ethnic variations in bioavailable vitamin D do not explain the lack of association between 25-hydroxyvitamin D and multiple sclerosis in blacks and Hispanics; and they further challenge the biological plausibility of vitamin D deficiency as causal for MS. PMID: 29414925
    14. We observed associations between VDR, GC, and CYP27B1 variants and maternal 25-hydroxyvitamin D concentration. Our results provide additional support for a possible role of genetic variation in vitamin D metabolism genes on vitamin D status during pregnancy. PMID: 29175129
    15. Data show that vitamin D-binding protein (DBP) is elevated in the CSF of temporal lobe epilepsy patients. PMID: 19109932
    16. In a Turkish Parkinson disease cohort, rs7041 of GC was associated with the PD risk. The homozygous major allele carriers for rs2282679, rs3755967 and rs2298850 of GC gene in PD patients with slower progression had significantly higher levels of serum 25OHD. This is the first study demonstrating GC gene as a risk factor for PD. PMID: 27282160
    17. Vitamin D levels are associated with severity of liver fibrosis in chronic hepatitis C genotype 1 patients. Although the rs7041 and rs4588 GC polymorphisms are strong predictors of vitamin D levels, they do not play a direct role in liver fibrosis. PMID: 28809744
    18. Vitamin D binding protein polymorphisms were frequently associated to fibrosis grade in chronic hepatitis C suggesting that they could be used as disease evaluation markers to understand the mechanisms underlying the virus-host interaction. PMID: 29465575
    19. Findings indicate that Gc globulin (GC) rs16847024, retinoid X receptor gamma (RXRG) rs17429130 and retinoid X receptor alpha (RXRA) rs4917356 were candidate susceptibility markers for gestational diabetes mellitus (GDM) in Chinese females. PMID: 27636996
    20. Increased circulating levels of vitamin D binding protein in multiple sclerosis patients. PMID: 25590278
    21. Results show that APOE, DBP and AGT identified were associated with survival outcomes in metastatic colorectal cancer patients treated with chemotherapy and bevacizumab PMID: 25428203
    22. results provide a direct evidence of cross-talk among the structural domains of DBP PMID: 18035050
    23. DBP strictly inhibited the production of 1alpha,25-dihydroxyvitamin D3 and 25-hydroxyvitamin D3-induced T cell responses. PMID: 25230725
    24. 25(OH)D2 half-life was shorter than 25(OH)D3 half-life, and half-lives were affected by DBP concentration and genotype PMID: 24885631
    25. The interaction between vitamin D status, as measured by circulating 25(OH)D and DBP rs2282679 genotypes, modified the association between 25(OH)D and BMD and bone markers PMID: 25890042
    26. Data suggest that, while low circulating levels of DBP contribute to low circulating levels of 25-hydroxyvitamin D in patients with PHPT (primary hyperparathyroidism), low DBP alone is not responsible for the hypovitaminosis D observed in these patients; vitamin D metabolism is likely to be generally disturbed in PHPT. [EDITORIAL] PMID: 27858283
    27. the purpose of this investigation was to assess the relative degree of O-linked trisaccharide glycosylation of DBP in breast, colorectal, pancreatic, and prostate cancer patients compared with healthy individuals. PMID: 19642159
    28. Comparison of Two ELISA Methods and Mass Spectrometry for Measurement of Vitamin D-Binding Protein: Implications for the Assessment of Bioavailable Vitamin D Concentrations Across Genotypes. PMID: 27250744
    29. Single-nucleotide polymorphisms in the vitamin D binding protein genewere independently associated with lower 25-hydroxy-vitamin Dand higher 24,25-dihydroxy-vitamin D. PMID: 27313313
    30. genetic association studies in a population in Brazil: Data suggest that SNPs in RXRG (rs2134095) and GC (rs7041) are associated with low-density lipoprotein cholesterol levels and hypercholesterolemia in the population studied; there was no apparent association with an SNP in VDR (rs2228570). (RXRG = retinoid X receptor gamma; GC = vitamin D-binding protein; VDR = vitamin D receptor) PMID: 27721113
    31. The study strongly suggests that there might have an association of vitamin D, and vitamin D-binding protein gene (codon 416 & 420) polymorphisms with the occurrence of type 2 diabetes mellitus PMID: 28888576
    32. Low serum vitamin D-binding protein concentrations are associated with type 1 diabetes. PMID: 27103201
    33. rs7041 polymorphism of Vitamin D Binding Protein does not affect platelet reactivity or the rate of high-residual platelet reactivity among patients receiving dual antiplatelet therapy with clopidogrel or ticagrelor. PMID: 28433569
    34. High VDBG concentration was associated with coronary heart disease events in all racial and ethnic groups. PMID: 28472285
    35. SNPs rs7041 and rs4588 of VDBP are not associated with the levels of 25-hydroxyvitamin D nor with the prevalence and extent of CAD. 25-hydroxyvitamin D levels but not VDBP genetic status independently predicted the occurrence of coronary lesions at angiography. PMID: 28779988
    36. Genetic variant in vitamin D-binding protein is associated with metabolic syndrome. PMID: 28278285
    37. Study suggests higher (versus lower) circulating DBP may be independently associated with a decreased prostate cancer risk in black men independent of 25(OH)D status. PMID: 28369777
    38. The data demonstrate a relationship between the diurnal rhythms of 1,25(OH)2D and DBP, possibly to maintain free 1,25(OH)2D concentrations. PMID: 28732681
    39. the interaction between 25(OH)D status and some maternal GC variants influence the birth weight of infants PMID: 28241988
    40. The results of this study suggest that DBP is not involved in the pathogenesis of MS in Italians. PMID: 28284354
    41. This report summerizes current understanding of vitamin D-binding protein, its genetic determinants, and their effect on calcifediol concentrations. (Review) PMID: 27742848
    42. Allelic variations in CYP2R1 and GC affect vitamin D levels, but variant alleles on VDR and DHCR7 were not correlated with vitamin D deficiency. PMID: 26038244
    43. Patients with primary hyperparathyroidism have lower serum VDBP than controls. PMID: 27682354
    44. VDBP polymorphism is associated with vitamin D deficiency. PMID: 27768857
    45. genome-wide association study in population of children/adolescents in Colorado: Data suggest that VDBP is an autoantigen in development of autoimmune type 1 diabetes (T1D) in the population studied; serum 25-hydroxyvitamin D levels are negatively correlated with VDBP-autoantibody levels in patients in whom T1D developed during the winter. [META-ANALYSIS] PMID: 26983959
    46. VDBP rs222020 C > T polymorphisms may be predisposition factors of adolescent idiopathic scoliosis and the efficacy of brace treatment. PMID: 27856225
    47. findings do not support a role of an independent effect of the investigated vitamin D-related gene variants, VDBP and CYP27B1, in the risk of Multiple Sclerosis PMID: 27904983
    48. The polymorphisms in the VDR and VDBP genes appeared to be responsible for host susceptibility to human tuberculosis in a Taiwanese population. PMID: 26869016
    49. No association was observed between GC or VDR polymorphisms and breast cancer risk. associations between vitamin D-related genetic variants and breast cancer were not observed overall, although the relationships between vitamin D pathway polymorphisms and breast cancer may be modified by menopausal status and breast tumour subtype PMID: 26631034
    50. In a Pakistani population, no statistically significant associations between SNPs in VDR, DBP, and CYP2R1 and tuberculosis was demonstrated. PMID: 27160686

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    ALB/AFP/VDB family
  • 组织特异性：
    Expressed in the liver. Found in plasma, ascites, cerebrospinal fluid and urine.
  • 数据库链接：

    HGNC: 4187

    OMIM: 139200

    KEGG: hsa:2638

    STRING: 9606.ENSP00000421725

    UniGene: Hs.418497