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  4. Recombinant Human WD repeat-containing protein 74 (WDR74)

Recombinant Human WD repeat-containing protein 74 (WDR74)

  • 货号：
    CSB-YP747557HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP747557HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP747557HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP747557HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP747557HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    WDR74
  • Uniprot No.：
  • 别名：
    WDR74; NSA1; WD repeat-containing protein 74; NOP seven-associated protein 1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-385
  • 氨基酸序列
    MAAAAARWNH VWVGTETGIL KGVNLQRKQA ANFTAGGQPR REEAVSALCW GTGGETQMLV GCADRTVKHF STEDGIFQGQ RHCPGGEGMF RGLAQADGTL ITCVDSGILR VWHDKDKDTS SDPLLELRVG PGVCRMRQDP AHPHVVATGG KENALKIWDL QGSEEPVFRA KNVRNDWLDL RVPIWDQDIQ FLPGSQKLVT CTGYHQVRVY DPASPQRRPV LETTYGEYPL TAMTLTPGGN SVIVGNTHGQ LAEIDLRQGR LLGCLKGLAG SVRGLQCHPS KPLLASCGLD RVLRIHRIQN PRGLEHKVYL KSQLNCLLLS GRDNWEDEPQ EPQEPNKVPL EDTETDELWA SLEAAAKRKL SGLEQPQGAL QTRRRKKKRP GSTSP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Regulatory protein of the MTREX-exosome complex involved in the synthesis of the 60S ribosomal subunit. Participates in an early cleavage of the pre-rRNA processing pathway in cooperation with NVL. Required for blastocyst formation, is necessary for RNA transcription, processing and/or stability during preimplantation development.
  • 基因功能参考文献：
    1. knockdown of WDR74 leads to significant defects in the pre-rRNA cleavage within the internal transcribed spacer 1, occurring in an early stage of the processing pathway. When the dissociation of WDR74 from the MTR4-containing exonuclease complex was impaired upon expression of mutant NVL2, the same processing defect, with partial migration of WDR74 from the nucleolus towards the nucleoplasm, was observed. PMID: 29107693
    2. results suggest that WDR74 is a novel regulatory protein of the MTR4-exsosome complex whose interaction is regulated by NVL2 and is involved in ribosome biogenesis PMID: 26456651
  • 亚细胞定位：
    Nucleus, nucleolus. Nucleus.
  • 数据库链接：

    HGNC: 25529

    OMIM: 617947

    KEGG: hsa:54663

    STRING: 9606.ENSP00000278856

    UniGene: Hs.654620